In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Jênifer Malheiros
Data de Publicação: 2022
Outros Autores: Cardoso, Thayná Laner, Freitas, Stella Buchhorn de, Woloski, Rafael, Seixas Neto, Amilton Clair Pinto, Pinto, Luciano da Silva, Lemos, Elba Sampaio de, Hartwig, Daiane Drawanz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56469
Resumo: Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil / Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.
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spelling Gonçalves, Jênifer MalheirosCardoso, Thayná LanerFreitas, Stella Buchhorn deWoloski, RafaelSeixas Neto, Amilton Clair PintoPinto, Luciano da SilvaLemos, Elba Sampaio deHartwig, Daiane Drawanz2023-01-15T13:40:42Z2023-01-15T13:40:42Z2022GONÇALVES, Jênifer Malheiros et al. In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease. Applied Microbiology and Biotechnology, v. 106, p.8079-8091, Nov. 2022.0175-7598https://www.arca.fiocruz.br/handle/icict/5646910.1007/s00253-022-12269-3engSpringerAnálise in silicoProteína recombinanteVacinaDiagnósticoIn silico analysisRecombinant proteinVaccineDiagnosisIn silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil / Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil .Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil / Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil .Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses E Rickettsioses (LHR). Rio de Janeiro, RJ, Brasil.Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil / Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil / Universidade Federal de Pelotas. Instituto de Biologia. Pelotas, RS, Brasil.Bartonella henselae is a Gram-negative bacterium that causes cat scratch disease (CSD), as well as bacteremia, endocarditis, and other clinical presentations. CSD remains one of the most common infections caused by bacteria in the genus Bartonella, and it is transmitted to humans through a scratch or cat bite. Vaccination and more efficient diagnostic methods would represent a promising and sustainable alternative measure for CSD control in humans and animals. Here, we described the in silico analyses and design of three recombinant chimeric proteins (rC1, rC2, and rC3), for use in the control of CSD. The chimeras were constructed with epitopes identified from the sequences of the GroEL, 17 kDa, P26, BadA, Pap31, OMP 89, and OMP 43, previously described as the most important B. henselae antigens. The rC1, rC2, and rC3 were expressed and purified using a heterologous system based on Escherichia coli and reacted with antibodies present in the sera of humans naturally infected. The chimeric proteins were used to immunize mice using Freund adjuvant, and the humoral immune response was evaluated. Animals immunized with rC1 and rC3 showed a significant IgG antibodies response from the 28th day (P < 0.05), and the animals immunized with the rC2 from the 35th day (P < 0.05) remained until the 56th day of experimentation, with a titer of 1:3200 (P < 0.05), 1:1600 (P < 0.05) and 1:1600 (P < 0.05) from rC1, rC2, and rC3, respectively. Significant production of IgA and IgG1 isotype was detected in animals immunized with rC1 and rC2 proteins. Additionally, analysis using 13 serum samples from naturally infected patients showed that the proteins are recognized by antibodies present in sera, reinforcing the possibility of using these chimeras for CSD control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56469/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJeniferGonçalves_ElbaRSampaio de Lemos_etal _IOC_2022.pdfJeniferGonçalves_ElbaRSampaio de Lemos_etal _IOC_2022.pdfapplication/pdf1197448https://www.arca.fiocruz.br/bitstream/icict/56469/2/JeniferGon%c3%a7alves_ElbaRSampaio%20de%20Lemos_etal%20_IOC_2022.pdf46f42eeb461d296a2b8ce7ada1c5ea30MD52icict/564692023-09-14 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dc.title.en_US.fl_str_mv In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
title In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
spellingShingle In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
Gonçalves, Jênifer Malheiros
Análise in silico
Proteína recombinante
Vacina
Diagnóstico
In silico analysis
Recombinant protein
Vaccine
Diagnosis
title_short In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
title_full In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
title_fullStr In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
title_full_unstemmed In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
title_sort In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease
author Gonçalves, Jênifer Malheiros
author_facet Gonçalves, Jênifer Malheiros
Cardoso, Thayná Laner
Freitas, Stella Buchhorn de
Woloski, Rafael
Seixas Neto, Amilton Clair Pinto
Pinto, Luciano da Silva
Lemos, Elba Sampaio de
Hartwig, Daiane Drawanz
author_role author
author2 Cardoso, Thayná Laner
Freitas, Stella Buchhorn de
Woloski, Rafael
Seixas Neto, Amilton Clair Pinto
Pinto, Luciano da Silva
Lemos, Elba Sampaio de
Hartwig, Daiane Drawanz
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Jênifer Malheiros
Cardoso, Thayná Laner
Freitas, Stella Buchhorn de
Woloski, Rafael
Seixas Neto, Amilton Clair Pinto
Pinto, Luciano da Silva
Lemos, Elba Sampaio de
Hartwig, Daiane Drawanz
dc.subject.other.en_US.fl_str_mv Análise in silico
Proteína recombinante
Vacina
Diagnóstico
topic Análise in silico
Proteína recombinante
Vacina
Diagnóstico
In silico analysis
Recombinant protein
Vaccine
Diagnosis
dc.subject.en.en_US.fl_str_mv In silico analysis
Recombinant protein
Vaccine
Diagnosis
description Universidade Federal de Pelotas. Instituto de Biologia. Departamento de Microbiologia E Parasitologia. Laboratório de Bacteriologia E Bioensaios (LaBBio). Capão do Leão, RS, Brasil / Universidade Federal Pelotas. de Desenvolvimento Tecnológico (CDTec). Núcleo de Biotecnologia. Capão do Leão, RS, Brasil.
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-01-15T13:40:42Z
dc.date.available.fl_str_mv 2023-01-15T13:40:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONÇALVES, Jênifer Malheiros et al. In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease. Applied Microbiology and Biotechnology, v. 106, p.8079-8091, Nov. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56469
dc.identifier.issn.en_US.fl_str_mv 0175-7598
dc.identifier.doi.none.fl_str_mv 10.1007/s00253-022-12269-3
identifier_str_mv GONÇALVES, Jênifer Malheiros et al. In silico analyses and design of chimeric proteins containing epitopes of Bartonella henselae antigens for the control of cat scratch disease. Applied Microbiology and Biotechnology, v. 106, p.8079-8091, Nov. 2022.
0175-7598
10.1007/s00253-022-12269-3
url https://www.arca.fiocruz.br/handle/icict/56469
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56469/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56469/2/JeniferGon%c3%a7alves_ElbaRSampaio%20de%20Lemos_etal%20_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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