Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism

Detalhes bibliográficos
Autor(a) principal: Toledo, Daniel A. M.
Data de Publicação: 2016
Outros Autores: Roque, Natália R., Teixeira, Lívia, Milán-Garcés, Erix A, Carneiro, Alan B., Almeida, Mariana R., Andrade, Gustavo F. S., Martins, Jefferson S., Pinho, Roberto R., Lima, Célio G. Freire de, Bozza, Patrícia T., D'Avila, Heloisa, Melo, Rossana C. N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17978
Resumo: Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.
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spelling Toledo, Daniel A. M.Roque, Natália R.Teixeira, LíviaMilán-Garcés, Erix ACarneiro, Alan B.Almeida, Mariana R.Andrade, Gustavo F. S.Martins, Jefferson S.Pinho, Roberto R.Lima, Célio G. Freire deBozza, Patrícia T.D'Avila, HeloisaMelo, Rossana C. N.2017-03-09T11:05:31Z2017-03-09T11:05:31Z2016TOLEDO, Daniel A. M. et al. Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism. Plos One, v.11, n.8, e0160433, 22p, Aug. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1797810.1371/journal.pone.0160433engPublic Library of ScienceTrypanosoma cruziÓrgãos do corpo lipídicoMetabolismo do ácido araquidônicoIntracelularDoença de ChagasTrypanosoma cruziLipid Body OrganellesArachidonic Acid MetabolismIntracellularChagas diseaseLipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo de Estrutura. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo de Estrutura. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo de Estrutura. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Física. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Física. Juiz de Fora, MG, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Most eukaryotic cells contain varying amounts of cytosolic lipidic inclusions termed lipid bodies (LBs) or lipid droplets (LDs). In mammalian cells, such as macrophages, these lipid-rich organelles are formed in response to host-pathogen interaction during infectious diseases and are sites for biosynthesis of arachidonic acid (AA)-derived inflammatory mediators (eicosanoids). Less clear are the functions of LBs in pathogenic lower eukaryotes. In this study, we demonstrated that LBs, visualized by light microscopy with different probes and transmission electron microscopy (TEM), are produced in trypomastigote forms of the parasite Trypanosoma cruzi, the causal agent of Chagas' disease, after both host interaction and exogenous AA stimulation. Quantitative TEM revealed that LBs from amastigotes, the intracellular forms of the parasite, growing in vivo have increased size and electron-density compared to LBs from amastigotes living in vitro. AA-stimulated trypomastigotes released high amounts of prostaglandin E2 (PGE2) and showed PGE2 synthase expression. Raman spectroscopy demonstrated increased unsaturated lipid content and AA incorporation in stimulated parasites. Moreover, both Raman and MALDI mass spectroscopy revealed increased AA content in LBs purified from AA-stimulated parasites compared to LBs from unstimulated group. By using a specific technique for eicosanoid detection, we immunolocalized PGE2 within LBs from AA-stimulated trypomastigotes. Altogether, our findings demonstrate that LBs from the parasite Trypanosoma cruzi are not just lipid storage inclusions but dynamic organelles, able to respond to host interaction and inflammatory events and involved in the AA metabolism. Acting as sources of PGE2, a potent immunomodulatory lipid mediator that inhibits many aspects of innate and adaptive immunity, newly-formed parasite LBs may be implicated with the pathogen survival in its host.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17978/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALlivia_teixeira_etal_IOC_2016.pdflivia_teixeira_etal_IOC_2016.pdfapplication/pdf9130355https://www.arca.fiocruz.br/bitstream/icict/17978/2/livia_teixeira_etal_IOC_2016.pdf55b9f60784f4edb78ef9d668727b9badMD52TEXTlivia_teixeira_etal_IOC_2016.pdf.txtlivia_teixeira_etal_IOC_2016.pdf.txtExtracted texttext/plain68443https://www.arca.fiocruz.br/bitstream/icict/17978/3/livia_teixeira_etal_IOC_2016.pdf.txt0e87b2aff7d9da721a5bf104d3602cfbMD53icict/179782023-09-14 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dc.title.pt_BR.fl_str_mv Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
title Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
spellingShingle Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
Toledo, Daniel A. M.
Trypanosoma cruzi
Órgãos do corpo lipídico
Metabolismo do ácido araquidônico
Intracelular
Doença de Chagas
Trypanosoma cruzi
Lipid Body Organelles
Arachidonic Acid Metabolism
Intracellular
Chagas disease
title_short Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
title_full Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
title_fullStr Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
title_full_unstemmed Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
title_sort Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism
author Toledo, Daniel A. M.
author_facet Toledo, Daniel A. M.
Roque, Natália R.
Teixeira, Lívia
Milán-Garcés, Erix A
Carneiro, Alan B.
Almeida, Mariana R.
Andrade, Gustavo F. S.
Martins, Jefferson S.
Pinho, Roberto R.
Lima, Célio G. Freire de
Bozza, Patrícia T.
D'Avila, Heloisa
Melo, Rossana C. N.
author_role author
author2 Roque, Natália R.
Teixeira, Lívia
Milán-Garcés, Erix A
Carneiro, Alan B.
Almeida, Mariana R.
Andrade, Gustavo F. S.
Martins, Jefferson S.
Pinho, Roberto R.
Lima, Célio G. Freire de
Bozza, Patrícia T.
D'Avila, Heloisa
Melo, Rossana C. N.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Toledo, Daniel A. M.
Roque, Natália R.
Teixeira, Lívia
Milán-Garcés, Erix A
Carneiro, Alan B.
Almeida, Mariana R.
Andrade, Gustavo F. S.
Martins, Jefferson S.
Pinho, Roberto R.
Lima, Célio G. Freire de
Bozza, Patrícia T.
D'Avila, Heloisa
Melo, Rossana C. N.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Órgãos do corpo lipídico
Metabolismo do ácido araquidônico
Intracelular
Doença de Chagas
topic Trypanosoma cruzi
Órgãos do corpo lipídico
Metabolismo do ácido araquidônico
Intracelular
Doença de Chagas
Trypanosoma cruzi
Lipid Body Organelles
Arachidonic Acid Metabolism
Intracellular
Chagas disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Lipid Body Organelles
Arachidonic Acid Metabolism
Intracellular
Chagas disease
description Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-09T11:05:31Z
dc.date.available.fl_str_mv 2017-03-09T11:05:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TOLEDO, Daniel A. M. et al. Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism. Plos One, v.11, n.8, e0160433, 22p, Aug. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17978
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0160433
identifier_str_mv TOLEDO, Daniel A. M. et al. Lipid Body Organelles within the Parasite Trypanosoma cruzi: A Role for Intracellular Arachidonic Acid Metabolism. Plos One, v.11, n.8, e0160433, 22p, Aug. 2016.
1932-6203
10.1371/journal.pone.0160433
url https://www.arca.fiocruz.br/handle/icict/17978
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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