Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells

Detalhes bibliográficos
Autor(a) principal: Palmeira, Vanila Faber
Data de Publicação: 2010
Outros Autores: Kneipp, Lucimar Ferreira, Alviano, Celuta Sales, Santos, André Luis Souza dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25713
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.
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spelling Palmeira, Vanila FaberKneipp, Lucimar FerreiraAlviano, Celuta SalesSantos, André Luis Souza dos2018-04-10T12:56:44Z2018-04-10T12:56:44Z2010PALMEIRA, Vanila Faber; et al. Phospholipase and Esterase Production by Clinical Strains of Fonsecaea pedrosoi and Their Interactions with Epithelial Cells. Mycopathologia, v.170, p.31–37, 2010.0301-486Xhttps://www.arca.fiocruz.br/handle/icict/2571310.1007/s11046-010-9293-61573-0832engSpringer Verlag (Germany)FosfolipasasEsteraseDiferenciaçãoComunicação Celularestirpes clínicasFonsecaea pedrosoiPhospholipaseEsteraseDifferentiationCellular interactionClinical strainsPhospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.Fonsecaea pedrosoi is the major etiologic agent of chromoblastomycosis. The virulence of F. pedrosoi is a meagerly explored phenomenon. The ability to interact with host cells and the production of hydrolytic enzymes are thought to be important virulence mechanisms of fungal pathogens. Here, we measured the production of two distinct lipolytic enzymes, phospholipase and esterase, by three clinical strains of F. pedrosoi isolated from chromoblastomycosis lesions, as well as their capabilities to interact with epithelial cells. All the strains were excellent esterase producers, generating elevated hydrolytic halos after 5 days of growth. Conversely, phospholipase activity was detected only after 10 days, except for the most recent strain of F. pedrosoi (Magé) in which measurable phospholipase activity was detected on day 5. The ability to interact with epithelial cells was also investigated. Regarding the adhesion capability, an indirect connection was observed in relation to the adaptation time of each strain in axenic culture, in which Magé strain showed the best adhesion ability followed by LDI 11428 and 5VPL strains. Both 5VPL and Magé strains were also detected inside the epithelial cells, while the LDI 11428 strain was rarely detected in cytoplasmatic vacuolar compartments. Moreover, these F. pedrosoi strains were able to cause injury in epithelial cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25713/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALlucimar3_kneipp_etal_IOC_2010.pdflucimar3_kneipp_etal_IOC_2010.pdfapplication/pdf363334https://www.arca.fiocruz.br/bitstream/icict/25713/2/lucimar3_kneipp_etal_IOC_2010.pdf6409e670511f05ad91f2728da660e763MD52TEXTlucimar3_kneipp_etal_IOC_2010.pdf.txtlucimar3_kneipp_etal_IOC_2010.pdf.txtExtracted texttext/plain29591https://www.arca.fiocruz.br/bitstream/icict/25713/3/lucimar3_kneipp_etal_IOC_2010.pdf.txt31d6f9177b0f77351d2700238cdbb7deMD53icict/257132018-08-15 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dc.title.pt_BR.fl_str_mv Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
title Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
spellingShingle Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
Palmeira, Vanila Faber
Fosfolipasas
Esterase
Diferenciação
Comunicação Celular
estirpes clínicas
Fonsecaea pedrosoi
Phospholipase
Esterase
Differentiation
Cellular interaction
Clinical strains
title_short Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
title_full Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
title_fullStr Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
title_full_unstemmed Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
title_sort Phospholipase and esterase production by clinical strains of Fonsecaea pedrosoi and their interactions with epithelial cells
author Palmeira, Vanila Faber
author_facet Palmeira, Vanila Faber
Kneipp, Lucimar Ferreira
Alviano, Celuta Sales
Santos, André Luis Souza dos
author_role author
author2 Kneipp, Lucimar Ferreira
Alviano, Celuta Sales
Santos, André Luis Souza dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Palmeira, Vanila Faber
Kneipp, Lucimar Ferreira
Alviano, Celuta Sales
Santos, André Luis Souza dos
dc.subject.other.pt_BR.fl_str_mv Fosfolipasas
Esterase
Diferenciação
Comunicação Celular
estirpes clínicas
topic Fosfolipasas
Esterase
Diferenciação
Comunicação Celular
estirpes clínicas
Fonsecaea pedrosoi
Phospholipase
Esterase
Differentiation
Cellular interaction
Clinical strains
dc.subject.en.pt_BR.fl_str_mv Fonsecaea pedrosoi
Phospholipase
Esterase
Differentiation
Cellular interaction
Clinical strains
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Microbiologia Geral. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-04-10T12:56:44Z
dc.date.available.fl_str_mv 2018-04-10T12:56:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PALMEIRA, Vanila Faber; et al. Phospholipase and Esterase Production by Clinical Strains of Fonsecaea pedrosoi and Their Interactions with Epithelial Cells. Mycopathologia, v.170, p.31–37, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25713
dc.identifier.issn.pt_BR.fl_str_mv 0301-486X
dc.identifier.doi.none.fl_str_mv 10.1007/s11046-010-9293-6
dc.identifier.eissn.none.fl_str_mv 1573-0832
identifier_str_mv PALMEIRA, Vanila Faber; et al. Phospholipase and Esterase Production by Clinical Strains of Fonsecaea pedrosoi and Their Interactions with Epithelial Cells. Mycopathologia, v.170, p.31–37, 2010.
0301-486X
10.1007/s11046-010-9293-6
1573-0832
url https://www.arca.fiocruz.br/handle/icict/25713
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag (Germany)
publisher.none.fl_str_mv Springer Verlag (Germany)
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25713/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/25713/2/lucimar3_kneipp_etal_IOC_2010.pdf
https://www.arca.fiocruz.br/bitstream/icict/25713/3/lucimar3_kneipp_etal_IOC_2010.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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