Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development

Detalhes bibliográficos
Autor(a) principal: Castro, Daniele P.
Data de Publicação: 2012
Outros Autores: Moraes, Caroline S., Gonzalez, Marcelo S., Ratcliffe, Norman A., Azambuja, Patrícia, Garcia, Eloi S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14649
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.
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spelling Castro, Daniele P.Moraes, Caroline S.Gonzalez, Marcelo S.Ratcliffe, Norman A.Azambuja, PatríciaGarcia, Eloi S.2016-06-23T19:44:42Z2016-07-07T12:00:48Z2016-06-23T19:44:42Z2016-07-07T12:00:48Z2012CASTRO, Daniele P. et al. Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development. Plos One, v.7, n.5, e36591. 8p, May 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1464910.1371/journal.pone.0036591engPublic Library of ScienceTrypanosoma cruziMicrobiotaIntestinoRespostas imunesParasitosTrypanosoma cruziRhodnius prolixusMicrobiotaGut immune responsesParasite infectionTrypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Developmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense (UFF). Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c, there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites. Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R. prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and these changes are crucial for the parasite development in the insect gut.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTdaniele_castro_etal_IOC_2012.PDF.txtdaniele_castro_etal_IOC_2012.PDF.txtExtracted texttext/plain45184https://www.arca.fiocruz.br/bitstream/icict/14649/4/daniele_castro_etal_IOC_2012.PDF.txt804f9a46fcecb64770eb86e2424c25aaMD54ORIGINALdaniele_castro_etal_IOC_2012.PDFapplication/pdf1299602https://www.arca.fiocruz.br/bitstream/icict/14649/2/daniele_castro_etal_IOC_2012.PDF00a4eaf4c23155bbc290def0183ad00dMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14649/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146492022-06-24 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
spellingShingle Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
Castro, Daniele P.
Trypanosoma cruzi
Microbiota
Intestino
Respostas imunes
Parasitos
Trypanosoma cruzi
Rhodnius prolixus
Microbiota
Gut immune responses
Parasite infection
title_short Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_full Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_fullStr Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_full_unstemmed Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
title_sort Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development
author Castro, Daniele P.
author_facet Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
author_role author
author2 Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castro, Daniele P.
Moraes, Caroline S.
Gonzalez, Marcelo S.
Ratcliffe, Norman A.
Azambuja, Patrícia
Garcia, Eloi S.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Microbiota
Intestino
Respostas imunes
Parasitos
topic Trypanosoma cruzi
Microbiota
Intestino
Respostas imunes
Parasitos
Trypanosoma cruzi
Rhodnius prolixus
Microbiota
Gut immune responses
Parasite infection
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Rhodnius prolixus
Microbiota
Gut immune responses
Parasite infection
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-06-23T19:44:42Z
2016-07-07T12:00:48Z
dc.date.available.fl_str_mv 2016-06-23T19:44:42Z
2016-07-07T12:00:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CASTRO, Daniele P. et al. Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development. Plos One, v.7, n.5, e36591. 8p, May 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14649
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0036591
identifier_str_mv CASTRO, Daniele P. et al. Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development. Plos One, v.7, n.5, e36591. 8p, May 2012.
1932-6203
10.1371/journal.pone.0036591
url https://www.arca.fiocruz.br/handle/icict/14649
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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