Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15081 |
Resumo: | Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. |
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Nascimento, Mateus LaguardiaBranco, Kelly Moreira Grillo RibeiroGasparini, Marcela RibeiroFerraz, Silvia GiannattasioLeite, Laura RabeloAraujo, Flávio Marcos GomesSalim, Anna Christina de MatosNicoli, Jacques RobertOliveira, Guilherme Corrêa deStancioli, Edel Figueiredo Barbosa2016-07-08T19:12:09Z2016-07-22T13:23:07Z2016-07-08T19:12:09Z2016-07-22T13:23:07Z2015NASCIMENTO, Mateus Laguardia et al. Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis. PLoS One, v. 10, n. 11, p. 1-19, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1508110.1371/journal.pone.0143294engPublic Library of ScienceVaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Understanding of microbial communities inhabiting cattle vaginal tract may lead to a better comprehension of bovine physiology and reproductive health being of great economic interest. Up to date, studies involving cattle microbiota are focused on the gastrointestinal tract, and little is known about the vaginal microbiota. This study aimed to investigate the vaginal microbiome in Nellore cattle, heifers and cows, pregnant and non-pregnant, using a culture independent approach. The main bacterial phyla found were Firmicutes (~40-50%), Bacteroidetes (~15-25%) and Proteobacteria (~5-25%), in addition to ~10-20% of non-classified bacteria. 45-55% of the samples were represented by only ten OTUs: Aeribacillus, Bacteroides, Clostridium, Ruminococcus, Rikenella, Alistipes, Bacillus, Eubacterium, Prevotella and non-classified bacteria. Interestingly, microbiota from all 20 animals could be grouped according to the respiratory metabolism of the main OTUs found, creating three groups of vaginal microbiota in cattle. Archaeal samples were dominated by the Methanobrevibacter genus (Euryarchaeota, ~55-70%). Ascomycota was the main fungal phylum (~80-95%) and Mycosphaerella the most abundant genus (~70-85%). Hormonal influence was not clear, but a tendency for the reduction of bacterial and increase of archaeal populations in pregnant animals was observed. Eukaryotes did not vary significantly between pregnant and non-pregnant animals, but tended to be more abundant on cows than on heifers. The present work describes a great microbial variability in the vaginal community among the evaluated animals and groups (heifers and cows, pregnant and non-pregnant), which is significantly different from the findings previously reported using culture dependent methods, pointing out the need for further studies on this issue. The microbiome found also indicates that the vaginal colonization appears to be influenced by the gastrointestinal community.ArchaeaBacteriaCattleFungiGeneticsClassificationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTve_Nascimento_Mateus_Vaginal_CPqRR_2015.PDF.txtve_Nascimento_Mateus_Vaginal_CPqRR_2015.PDF.txtExtracted texttext/plain53926https://www.arca.fiocruz.br/bitstream/icict/15081/4/ve_Nascimento_Mateus_Vaginal_CPqRR_2015.PDF.txt9e7bb84c35253b6e2f0c6ccf9b6989a2MD54ORIGINALve_Nascimento_Mateus_Vaginal_CPqRR_2015.PDFapplication/pdf3959212https://www.arca.fiocruz.br/bitstream/icict/15081/2/ve_Nascimento_Mateus_Vaginal_CPqRR_2015.PDFfeaa45e6e45dcdffbe1f4c24ab16bfabMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/15081/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/150812019-09-26 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| dc.title.pt_BR.fl_str_mv |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| title |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| spellingShingle |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis Nascimento, Mateus Laguardia Archaea Bacteria Cattle Fungi Genetics Classification |
| title_short |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| title_full |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| title_fullStr |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| title_full_unstemmed |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| title_sort |
Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis |
| author |
Nascimento, Mateus Laguardia |
| author_facet |
Nascimento, Mateus Laguardia Branco, Kelly Moreira Grillo Ribeiro Gasparini, Marcela Ribeiro Ferraz, Silvia Giannattasio Leite, Laura Rabelo Araujo, Flávio Marcos Gomes Salim, Anna Christina de Matos Nicoli, Jacques Robert Oliveira, Guilherme Corrêa de Stancioli, Edel Figueiredo Barbosa |
| author_role |
author |
| author2 |
Branco, Kelly Moreira Grillo Ribeiro Gasparini, Marcela Ribeiro Ferraz, Silvia Giannattasio Leite, Laura Rabelo Araujo, Flávio Marcos Gomes Salim, Anna Christina de Matos Nicoli, Jacques Robert Oliveira, Guilherme Corrêa de Stancioli, Edel Figueiredo Barbosa |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nascimento, Mateus Laguardia Branco, Kelly Moreira Grillo Ribeiro Gasparini, Marcela Ribeiro Ferraz, Silvia Giannattasio Leite, Laura Rabelo Araujo, Flávio Marcos Gomes Salim, Anna Christina de Matos Nicoli, Jacques Robert Oliveira, Guilherme Corrêa de Stancioli, Edel Figueiredo Barbosa |
| dc.subject.en.pt_BR.fl_str_mv |
Archaea Bacteria Cattle Fungi Genetics Classification |
| topic |
Archaea Bacteria Cattle Fungi Genetics Classification |
| description |
Universidade Federal Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil. |
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2015 |
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2015 |
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2016-07-08T19:12:09Z 2016-07-22T13:23:07Z |
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2016-07-08T19:12:09Z 2016-07-22T13:23:07Z |
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NASCIMENTO, Mateus Laguardia et al. Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis. PLoS One, v. 10, n. 11, p. 1-19, 2015. |
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https://www.arca.fiocruz.br/handle/icict/15081 |
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1932-6203 |
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10.1371/journal.pone.0143294 |
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NASCIMENTO, Mateus Laguardia et al. Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis. PLoS One, v. 10, n. 11, p. 1-19, 2015. 1932-6203 10.1371/journal.pone.0143294 |
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