Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.

Detalhes bibliográficos
Autor(a) principal: Costa, Patrícia Silva
Data de Publicação: 2015
Outros Autores: Guimarães, Mariana de Paula Reis, Ávila, Marcelo de Paula, Leite, Laura Rabelo, Araújo, Flávio Marcos Gomes de, Salim, Anna Christina de Matos, Oliveira, Guilherme Correa de, Barbosa, Francisco, Souza, Edmar Chartone, Nascimento, Andrea Maria Amaral
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12642
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil
id CRUZ_ded1bf8d72fb6a9d2b4c9ce040f05eef
oai_identifier_str oai:www.arca.fiocruz.br:icict/12642
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Costa, Patrícia SilvaGuimarães, Mariana de Paula ReisÁvila, Marcelo de PaulaLeite, Laura RabeloAraújo, Flávio Marcos Gomes deSalim, Anna Christina de MatosOliveira, Guilherme Correa deBarbosa, FranciscoSouza, Edmar ChartoneNascimento, Andrea Maria Amaral2016-01-28T12:50:41Z2016-01-28T12:50:41Z2015COSTA, Patrícia Silva et al. Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment. PLoS One, vol. 10, n. 3, p. e0119465, 2015.1932-620310.1371/journal.pone.0119465.https://www.arca.fiocruz.br/handle/icict/12642engPublic Library of ScienceMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilHere, we describe the metagenome and functional composition of a microbial community in a historically metal-contaminated tropical freshwater stream sediment. The sediment was collected from the Mina Stream located in the Iron Quadrangle (Brazil), one of the world's largest mining regions. Environmental DNA was extracted and was sequenced using SOLiD technology, and a total of 7.9 Gbp was produced. A taxonomic profile that was obtained by comparison to the Greengenes database revealed a complex microbial community with a dominance of Proteobacteria and Parvarcheota. Contigs were recruited by bacterial and archaeal genomes, especially Candidatus Nitrospira defluvii and Nitrosopumilus maritimus, and their presence implicated them in the process of N cycling in the Mina Stream sediment (MSS). Functional reconstruction revealed a large, diverse set of genes for ammonium assimilation and ammonification. These processes have been implicated in the maintenance of the N cycle and the health of the sediment. SEED subsystems functional annotation unveiled a high degree of diversity of metal resistance genes, suggesting that the prokaryotic community is adapted to metal contamination. Furthermore, a high metabolic diversity was detected in the MSS, suggesting that the historical arsenic contamination is no longer affecting the prokaryotic community. These results expand the current knowledge of the microbial taxonomic and functional composition of tropical metal-contaminated freshwater sediments.Archaea/classificationArchaea/isolation & purificationEnvironmental Microbiology MetagenomeProteobacteria/geneticsSequence Analysis, RNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12642/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.pdfMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.pdfapplication/pdf4222660https://www.arca.fiocruz.br/bitstream/icict/12642/2/Metagenome%20of%20a%20Microbial%20Community%20Inhabiting%20a%20Metal-Rich%20Tropical%20Stream%20Sediment.pdfef1ca00949c9dc5a12c9ccbd2705c4baMD52TEXTMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.pdf.txtMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.pdf.txtExtracted texttext/plain742https://www.arca.fiocruz.br/bitstream/icict/12642/3/Metagenome%20of%20a%20Microbial%20Community%20Inhabiting%20a%20Metal-Rich%20Tropical%20Stream%20Sediment.pdf.txt7385ffad0d14c0c1e677da9cfdc13395MD53icict/126422019-06-19 10:07:07.101oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
title Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
spellingShingle Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
Costa, Patrícia Silva
Archaea/classification
Archaea/isolation & purification
Environmental Microbiology Metagenome
Proteobacteria/genetics
Sequence Analysis, RNA
title_short Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
title_full Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
title_fullStr Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
title_full_unstemmed Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
title_sort Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
author Costa, Patrícia Silva
author_facet Costa, Patrícia Silva
Guimarães, Mariana de Paula Reis
Ávila, Marcelo de Paula
Leite, Laura Rabelo
Araújo, Flávio Marcos Gomes de
Salim, Anna Christina de Matos
Oliveira, Guilherme Correa de
Barbosa, Francisco
Souza, Edmar Chartone
Nascimento, Andrea Maria Amaral
author_role author
author2 Guimarães, Mariana de Paula Reis
Ávila, Marcelo de Paula
Leite, Laura Rabelo
Araújo, Flávio Marcos Gomes de
Salim, Anna Christina de Matos
Oliveira, Guilherme Correa de
Barbosa, Francisco
Souza, Edmar Chartone
Nascimento, Andrea Maria Amaral
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Patrícia Silva
Guimarães, Mariana de Paula Reis
Ávila, Marcelo de Paula
Leite, Laura Rabelo
Araújo, Flávio Marcos Gomes de
Salim, Anna Christina de Matos
Oliveira, Guilherme Correa de
Barbosa, Francisco
Souza, Edmar Chartone
Nascimento, Andrea Maria Amaral
dc.subject.en.pt_BR.fl_str_mv Archaea/classification
Archaea/isolation & purification
Environmental Microbiology Metagenome
Proteobacteria/genetics
Sequence Analysis, RNA
topic Archaea/classification
Archaea/isolation & purification
Environmental Microbiology Metagenome
Proteobacteria/genetics
Sequence Analysis, RNA
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-28T12:50:41Z
dc.date.available.fl_str_mv 2016-01-28T12:50:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Patrícia Silva et al. Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment. PLoS One, vol. 10, n. 3, p. e0119465, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12642
dc.identifier.issn.none.fl_str_mv 1932-6203
10.1371/journal.pone.0119465.
identifier_str_mv COSTA, Patrícia Silva et al. Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment. PLoS One, vol. 10, n. 3, p. e0119465, 2015.
1932-6203
10.1371/journal.pone.0119465.
url https://www.arca.fiocruz.br/handle/icict/12642
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12642/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12642/2/Metagenome%20of%20a%20Microbial%20Community%20Inhabiting%20a%20Metal-Rich%20Tropical%20Stream%20Sediment.pdf
https://www.arca.fiocruz.br/bitstream/icict/12642/3/Metagenome%20of%20a%20Microbial%20Community%20Inhabiting%20a%20Metal-Rich%20Tropical%20Stream%20Sediment.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
ef1ca00949c9dc5a12c9ccbd2705c4ba
7385ffad0d14c0c1e677da9cfdc13395
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325097379921920

Registros relacionados