Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23267 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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Barros, Maria Paloma S.França, Camila T.Lins, Rosanny Holanda F. B.Santos, Milena Danda V.Silva, Ednaldo J.Oliveira, Maria Betânia M.Silveira-Filho, Vladimir M.Rezende, Antônio M.Balbino, Valdir Q.Leal-Balbino, Tereza Cristina2017-11-20T12:28:40Z2017-11-20T12:28:40Z2014BARROS, Maria Paloma S. et al. Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains. PloS One, v. 9, n. 9, p. e108353, 2014.1932-6203https://www.arca.fiocruz.br/handle/icict/2326710.1371/journal.pone.0108353engDNA BacterianoDNA IntergênicoAnálise de Sequência de DNAGenoma BacterianoGenéticaYersinia pseudotuberculosisDNA, BacterialDNA, IntergenicYersinia pseudotuberculosisSequence Analysis, DNAGenome, BacterialGeneticsRepetições Palindrômicas Curtas Agrupadas e Regularmente EspaçadasgenéticaSequência de BasesBrasilVariação GenéticaDados de Sequência MolecularYersinia pestisclassificaçãoGenótipoFilogeniaPestemicrobiologiaDynamics of CRISPR loci in microevolutionary process of Yersinia pestis strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution of 146 Yersinia pestis strains from different biovars and locations was investigated in this work. The majority of strains from the Orientalis biovar presented specific spacer arrays, allowing for the establishment of a CRISPR signature for their respective isolates. Twenty-one new spacers were found in the Y. pestis strains from plague foci in Brazil. Ninety-three (64%) strains were grouped in the G1 genotype, whereas the others were distributed in 35 genotypes. This study allowed observing a microevolutionary process in a group of Y. pestis isolated from Brazil. We also identified specific genotypes of Y. pestis that were important for the establishment of the bacteria in plague foci in Brazil. The data have provided supporting evidence for the diversity and dynamics of CRISPR loci present in the genome of Y. pestis strains from plague foci in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23267/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. Dynamics of CRISPR - Barros.PDFart. Dynamics of CRISPR - Barros.PDFapplication/pdf867167https://www.arca.fiocruz.br/bitstream/icict/23267/2/art.%20Dynamics%20of%20CRISPR%20-%20Barros.PDF164a8bc3f0eba383aca1b17e4fc75a22MD52TEXTart. Dynamics of CRISPR - Barros.PDF.txtart. Dynamics of CRISPR - Barros.PDF.txtExtracted texttext/plain30700https://www.arca.fiocruz.br/bitstream/icict/23267/3/art.%20Dynamics%20of%20CRISPR%20-%20Barros.PDF.txt7702fa0f839893033c1eb1b6d20aee80MD53icict/232672018-08-15 02:41:01.176oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:41:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| title |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| spellingShingle |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains Barros, Maria Paloma S. DNA Bacteriano DNA Intergênico Análise de Sequência de DNA Genoma Bacteriano Genética Yersinia pseudotuberculosis DNA, Bacterial DNA, Intergenic Yersinia pseudotuberculosis Sequence Analysis, DNA Genome, Bacterial Genetics Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas genética Sequência de Bases Brasil Variação Genética Dados de Sequência Molecular Yersinia pestis classificação Genótipo Filogenia Peste microbiologia |
| title_short |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| title_full |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| title_fullStr |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| title_full_unstemmed |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| title_sort |
Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains |
| author |
Barros, Maria Paloma S. |
| author_facet |
Barros, Maria Paloma S. França, Camila T. Lins, Rosanny Holanda F. B. Santos, Milena Danda V. Silva, Ednaldo J. Oliveira, Maria Betânia M. Silveira-Filho, Vladimir M. Rezende, Antônio M. Balbino, Valdir Q. Leal-Balbino, Tereza Cristina |
| author_role |
author |
| author2 |
França, Camila T. Lins, Rosanny Holanda F. B. Santos, Milena Danda V. Silva, Ednaldo J. Oliveira, Maria Betânia M. Silveira-Filho, Vladimir M. Rezende, Antônio M. Balbino, Valdir Q. Leal-Balbino, Tereza Cristina |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barros, Maria Paloma S. França, Camila T. Lins, Rosanny Holanda F. B. Santos, Milena Danda V. Silva, Ednaldo J. Oliveira, Maria Betânia M. Silveira-Filho, Vladimir M. Rezende, Antônio M. Balbino, Valdir Q. Leal-Balbino, Tereza Cristina |
| dc.subject.other.pt_BR.fl_str_mv |
DNA Bacteriano DNA Intergênico Análise de Sequência de DNA Genoma Bacteriano Genética Yersinia pseudotuberculosis |
| topic |
DNA Bacteriano DNA Intergênico Análise de Sequência de DNA Genoma Bacteriano Genética Yersinia pseudotuberculosis DNA, Bacterial DNA, Intergenic Yersinia pseudotuberculosis Sequence Analysis, DNA Genome, Bacterial Genetics Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas genética Sequência de Bases Brasil Variação Genética Dados de Sequência Molecular Yersinia pestis classificação Genótipo Filogenia Peste microbiologia |
| dc.subject.en.pt_BR.fl_str_mv |
DNA, Bacterial DNA, Intergenic Yersinia pseudotuberculosis Sequence Analysis, DNA Genome, Bacterial Genetics |
| dc.subject.decs.pt_BR.fl_str_mv |
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas genética Sequência de Bases Brasil Variação Genética Dados de Sequência Molecular Yersinia pestis classificação Genótipo Filogenia Peste microbiologia |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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2014 |
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2014 |
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2017-11-20T12:28:40Z |
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2017-11-20T12:28:40Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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BARROS, Maria Paloma S. et al. Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains. PloS One, v. 9, n. 9, p. e108353, 2014. |
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https://www.arca.fiocruz.br/handle/icict/23267 |
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1932-6203 |
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10.1371/journal.pone.0108353 |
| identifier_str_mv |
BARROS, Maria Paloma S. et al. Dynamics of CRISPR loci in microevolutionary process of Yersinia pestis strains. PloS One, v. 9, n. 9, p. e108353, 2014. 1932-6203 10.1371/journal.pone.0108353 |
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