Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44918 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
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Almeida, Marcos AbreuAlmeida-Paes, RodrigoGuimarães, Allan JeffersonValente, Richard HemmiSoares, Célia Maria de AlmeidaZancopé-Oliveira, Rosely Maria2020-12-15T19:26:34Z2020-12-15T19:26:34Z2020ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020.2235-2988https://www.arca.fiocruz.br/handle/icict/4491810.3389/fcimb.2020.591121engFrontiers MediaImmunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Histoplasmosis is one of the most frequent systemic mycosis in HIV patients. In these patients, histoplasmosis has high rates of morbidity/mortality if diagnosis and treatment are delayed. Despite its relevance, there is a paucity of information concerning the interaction between Histoplasma capsulatum and the human host, especially regarding the B-cell response, which has a direct impact on the diagnosis. Culture-based "gold-standard" methods have limitations, making immunodiagnostic tests an attractive option for clinical decisions. Despite the continuous development of those tests, improving serological parameters is necessary to make these methods efficient tools for definitive diagnosis of histoplasmosis. This includes the determination of more specific and immunogenic antigens to improve specificity and sensitivity of assays. In this study, we performed a co-immunoprecipitation assay between a protein extract from the yeast form of H. capsulatum and pooled sera from patients with proven histoplasmosis, followed by shotgun mass spectrometry identification of antigenic targets. Sera from patients with other pulmonary infections or from healthy individuals living in endemic areas of histoplasmosis were also assayed to determine potentially cross-reactive proteins. The primary structures of H. capsulatum immunoprecipitated proteins were evaluated using the DNAStar Protean 7.0 software. In parallel, the online epitope prediction server, BCPREDS, was used to complement the B-epitope prediction analysis. Our approach detected 132 reactive proteins to antibodies present in histoplasmosis patients' sera. Among these antigens, 127 were recognized also by antibodies in heterologous patients' and/or normal healthy donors' sera. Therefore, the only three antigens specifically recognized by antibodies of histoplasmosis patients were mapped as potential antigenic targets: the M antigen, previously demonstrated in the diagnosis of histoplasmosis, and the catalase P and YPS-3 proteins, characterized as virulence factors of H. capsulatum, with antigenic properties still unclear. The other two proteins were fragments of the YPS-3 and M antigen. Overlapping results obtained from the two aforementioned bioinformatic tools, 16 regions from these three proteins are proposed as putative B-cell epitopes exclusive to H. capsulatum. These data reveal a new role for these proteins on H. capsulatum interactions with the immune system and indicate their possible use in new methods for the diagnosis of histoplasmosis.Histoplasma capsulatumHomo sapiensM antigenYPS-3Catalase PEpitopesImmunoproteomeMass spectrometryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/44918/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdfImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdfapplication/pdf1794439https://www.arca.fiocruz.br/bitstream/icict/44918/2/Immunoproteomics_Marcos_Almeida_etal_INI_2020.pdf28b232c76941c0c94169fe8aa81c7b04MD52TEXTImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdf.txtImmunoproteomics_Marcos_Almeida_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
title |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
spellingShingle |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis Almeida, Marcos Abreu Histoplasma capsulatum Homo sapiens M antigen YPS-3 Catalase P Epitopes Immunoproteome Mass spectrometry |
title_short |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
title_full |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
title_fullStr |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
title_full_unstemmed |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
title_sort |
Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens–Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis |
author |
Almeida, Marcos Abreu |
author_facet |
Almeida, Marcos Abreu Almeida-Paes, Rodrigo Guimarães, Allan Jefferson Valente, Richard Hemmi Soares, Célia Maria de Almeida Zancopé-Oliveira, Rosely Maria |
author_role |
author |
author2 |
Almeida-Paes, Rodrigo Guimarães, Allan Jefferson Valente, Richard Hemmi Soares, Célia Maria de Almeida Zancopé-Oliveira, Rosely Maria |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Marcos Abreu Almeida-Paes, Rodrigo Guimarães, Allan Jefferson Valente, Richard Hemmi Soares, Célia Maria de Almeida Zancopé-Oliveira, Rosely Maria |
dc.subject.en.pt_BR.fl_str_mv |
Histoplasma capsulatum Homo sapiens M antigen YPS-3 Catalase P Epitopes Immunoproteome Mass spectrometry |
topic |
Histoplasma capsulatum Homo sapiens M antigen YPS-3 Catalase P Epitopes Immunoproteome Mass spectrometry |
description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-12-15T19:26:34Z |
dc.date.available.fl_str_mv |
2020-12-15T19:26:34Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44918 |
dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
dc.identifier.doi.none.fl_str_mv |
10.3389/fcimb.2020.591121 |
identifier_str_mv |
ALMEIDA, Marcos Abreu et al. Immunoproteomics Reveals Pathogen's Antigens Involved in Homo sapiens-Histoplasma capsulatum Interaction and Specific Linear B-Cell Epitopes in Histoplasmosis. Frontiers in Cellular and Infection Microbiology, v. 10, p. 1-12, 2020. 2235-2988 10.3389/fcimb.2020.591121 |
url |
https://www.arca.fiocruz.br/handle/icict/44918 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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