The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity

Detalhes bibliográficos
Autor(a) principal: Garver, Lindsey S.
Data de Publicação: 2013
Outros Autores: Oliveira, Giselle de Almeida, Barillas-Mury, Carolina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50538
Resumo: NATIONAL INSTITUTES OF HEALTH
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spelling Garver, Lindsey S.Oliveira, Giselle de AlmeidaBarillas-Mury, Carolina2021-12-28T13:54:40Z2021-12-28T13:54:40Z2013GARVER, Lindsey S.; OLIVEIRA, Giselle de Almeida; BARILLAS-MURY, Carolina. The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity. PLoS Pathog., v. 9, n. 9, e1003622, 2013. doi: 10.1371/journal.ppat.10036221553-7374https://www.arca.fiocruz.br/handle/icict/5053810.1371/journal.ppat.1003622NATIONAL INSTITUTES OF HEALTHLaboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases.National Institutes of Health. Rockville, Maryland, United States of AmericaFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil./Laboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases.National Institutes of Health. Rockville, Maryland, United States of AmericaLaboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases.National Institutes of Health. Rockville, Maryland, United States of AmericaThe innate immune system of Anopheles gambiae mosquitoes limits Plasmodium infection through multiple molecular mechanisms. For example, midgut invasion by the parasite triggers an epithelial nitration response that promotes activation of the complement-like system. We found that suppression of the JNK pathway, by silencing either Hep, JNK, Jun or Fos expression, greatly enhanced Plasmodium infection; while overactivating this cascade, by silencing the suppressor Puckered, had the opposite effect. The JNK pathway limits infection via two coordinated responses. It induces the expression of two enzymes (HPx2 and NOX5) that potentiate midgut epithelial nitration in response to Plasmodium infection and regulates expression of two key hemocyte-derived immune effectors (TEP1 and FBN9). Furthermore, the An. gambiae L3-5 strain that has been genetically selected to be refractory (R) to Plasmodium infection exhibits constitutive overexpression of genes from the JNK pathway, as well as midgut and hemocyte effector genes. Silencing experiments confirmed that this cascade mediates, to a large extent, the drastic parasite elimination phenotype characteristic of this mosquito strain. In sum, these studies revealed the JNK pathway as a key regulator of the ability of An. gambiae mosquitoes to limit Plasmodium infection and identified several effector genes mediating these responses.engPublic Library of ScienceThe JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlemosquitoesAnopheles gambiaeMalariainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/50538/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALThe JNK Pathway Is a Key Mediator of Anopheles.pdfThe JNK Pathway Is a Key Mediator of Anopheles.pdfapplication/pdf1126165https://www.arca.fiocruz.br/bitstream/icict/50538/2/The%20JNK%20Pathway%20Is%20a%20Key%20Mediator%20of%20Anopheles.pdf7f5579897b783fd2ec240e5f47bab114MD52icict/505382021-12-28 10:54:40.016oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-12-28T13:54:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
title The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
spellingShingle The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
Garver, Lindsey S.
mosquitoes
Anopheles gambiae
Malaria
title_short The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
title_full The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
title_fullStr The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
title_full_unstemmed The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
title_sort The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity
author Garver, Lindsey S.
author_facet Garver, Lindsey S.
Oliveira, Giselle de Almeida
Barillas-Mury, Carolina
author_role author
author2 Oliveira, Giselle de Almeida
Barillas-Mury, Carolina
author2_role author
author
dc.contributor.author.fl_str_mv Garver, Lindsey S.
Oliveira, Giselle de Almeida
Barillas-Mury, Carolina
dc.subject.en.pt_BR.fl_str_mv mosquitoes
Anopheles gambiae
Malaria
topic mosquitoes
Anopheles gambiae
Malaria
description NATIONAL INSTITUTES OF HEALTH
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2021-12-28T13:54:40Z
dc.date.available.fl_str_mv 2021-12-28T13:54:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GARVER, Lindsey S.; OLIVEIRA, Giselle de Almeida; BARILLAS-MURY, Carolina. The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity. PLoS Pathog., v. 9, n. 9, e1003622, 2013. doi: 10.1371/journal.ppat.1003622
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50538
dc.identifier.issn.pt_BR.fl_str_mv 1553-7374
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.1003622
identifier_str_mv GARVER, Lindsey S.; OLIVEIRA, Giselle de Almeida; BARILLAS-MURY, Carolina. The JNK Pathway Is a Key Mediator of Anopheles gambiae Antiplasmodial Immunity. PLoS Pathog., v. 9, n. 9, e1003622, 2013. doi: 10.1371/journal.ppat.1003622
1553-7374
10.1371/journal.ppat.1003622
url https://www.arca.fiocruz.br/handle/icict/50538
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50538/1/license.txt
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