Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos

Detalhes bibliográficos
Autor(a) principal: Manso, Pedro Paulo de Abreu
Data de Publicação: 2015
Outros Autores: Oliveira, Barbara C. E. P. Dias de, Sequeira, Patrícia Carvalho de, Souza, Yuli Rodrigues Maia de, Ferro, Jessica Maria dos Santos, Silva, Igor José da, Caputo, Luzia Fátima Gonçalves, Guedes, Priscila Tavares, Santos, Alexandre Araujo Cunha dos, Freire, Marcos da Silva, Bonaldo, Myrna Cristina, Machado, Marcelo Pelajo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14440
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
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spelling Manso, Pedro Paulo de AbreuOliveira, Barbara C. E. P. Dias deSequeira, Patrícia Carvalho deSouza, Yuli Rodrigues Maia deFerro, Jessica Maria dos SantosSilva, Igor José daCaputo, Luzia Fátima GonçalvesGuedes, Priscila TavaresSantos, Alexandre Araujo Cunha dosFreire, Marcos da SilvaBonaldo, Myrna CristinaMachado, Marcelo Pelajo2016-06-02T13:08:57Z2016-06-03T12:34:20Z2016-06-02T13:08:57Z2016-06-03T12:34:20Z2015MANSO, Pedro Paulo de Abreu; et al. Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. PLoS Negl Trop Dis., v.9, n.9, 16p, Sept. 2015.1935-2727https://www.arca.fiocruz.br/handle/icict/1444010.1371/journal.pntd.00040641935-2735engPublic Library of ScienceFebre AmarelaVacina 17DDInfecção pelo vírusEmbriões de frangosYellow Fever17DD VaccineVirus InfectionChicken EmbryosYellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Estado do Rio de Janeiro. UNIRIO. Rio de Janeiro, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created. The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated with the YF 17D virus. Yet, little information is available about the infection mechanism of YF 17DD virus in this biological model. To better understand this mechanism, we infected embryos of Gallus gallus domesticus and analyzed their histopathology after 72 hours of YF infection. Some embryos showed few apoptotic bodies in infected tissues, suggesting mild focal infection processes. Confocal and super-resolution microscopic analysis allowed us to identify as targets of viral infection: skeletal muscle cells, cardiomyocytes, nervous system cells, renal tubular epithelium, lung parenchyma, and fibroblasts associated with connective tissue in the perichondrium and dermis. The virus replication was heaviest in muscle tissues. In all of these specimens, RT-PCR methods confirmed the presence of replicative intermediate and genomic YF RNA. This clearer characterization of cell targets in chicken embryos paves the way for future development of a new YF vaccine based on a new cell culture system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALbarbara_oliveira_etal_IOC_2015.PDFapplication/pdf23209973https://www.arca.fiocruz.br/bitstream/icict/14440/1/barbara_oliveira_etal_IOC_2015.PDFeffc89d2bc8960bf65b9af114d5e8468MD51LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14440/2/license.txt5a560609d32a3863062d77ff32785d58MD52TEXTbarbara_oliveira_etal_IOC_2015.PDF.txtbarbara_oliveira_etal_IOC_2015.PDF.txtExtracted texttext/plain52522https://www.arca.fiocruz.br/bitstream/icict/14440/3/barbara_oliveira_etal_IOC_2015.PDF.txt0f5345d17d5c14590f5cf61dc3f2e8c6MD53icict/144402022-06-24 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dc.title.pt_BR.fl_str_mv Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
title Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
spellingShingle Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
Manso, Pedro Paulo de Abreu
Febre Amarela
Vacina 17DD
Infecção pelo vírus
Embriões de frangos
Yellow Fever
17DD Vaccine
Virus Infection
Chicken Embryos
title_short Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
title_full Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
title_fullStr Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
title_full_unstemmed Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
title_sort Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos
author Manso, Pedro Paulo de Abreu
author_facet Manso, Pedro Paulo de Abreu
Oliveira, Barbara C. E. P. Dias de
Sequeira, Patrícia Carvalho de
Souza, Yuli Rodrigues Maia de
Ferro, Jessica Maria dos Santos
Silva, Igor José da
Caputo, Luzia Fátima Gonçalves
Guedes, Priscila Tavares
Santos, Alexandre Araujo Cunha dos
Freire, Marcos da Silva
Bonaldo, Myrna Cristina
Machado, Marcelo Pelajo
author_role author
author2 Oliveira, Barbara C. E. P. Dias de
Sequeira, Patrícia Carvalho de
Souza, Yuli Rodrigues Maia de
Ferro, Jessica Maria dos Santos
Silva, Igor José da
Caputo, Luzia Fátima Gonçalves
Guedes, Priscila Tavares
Santos, Alexandre Araujo Cunha dos
Freire, Marcos da Silva
Bonaldo, Myrna Cristina
Machado, Marcelo Pelajo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Manso, Pedro Paulo de Abreu
Oliveira, Barbara C. E. P. Dias de
Sequeira, Patrícia Carvalho de
Souza, Yuli Rodrigues Maia de
Ferro, Jessica Maria dos Santos
Silva, Igor José da
Caputo, Luzia Fátima Gonçalves
Guedes, Priscila Tavares
Santos, Alexandre Araujo Cunha dos
Freire, Marcos da Silva
Bonaldo, Myrna Cristina
Machado, Marcelo Pelajo
dc.subject.other.pt_BR.fl_str_mv Febre Amarela
Vacina 17DD
Infecção pelo vírus
Embriões de frangos
topic Febre Amarela
Vacina 17DD
Infecção pelo vírus
Embriões de frangos
Yellow Fever
17DD Vaccine
Virus Infection
Chicken Embryos
dc.subject.en.pt_BR.fl_str_mv Yellow Fever
17DD Vaccine
Virus Infection
Chicken Embryos
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-06-02T13:08:57Z
2016-06-03T12:34:20Z
dc.date.available.fl_str_mv 2016-06-02T13:08:57Z
2016-06-03T12:34:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MANSO, Pedro Paulo de Abreu; et al. Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. PLoS Negl Trop Dis., v.9, n.9, 16p, Sept. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14440
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0004064
dc.identifier.eissn.pt_BR.fl_str_mv 1935-2735
identifier_str_mv MANSO, Pedro Paulo de Abreu; et al. Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos. PLoS Negl Trop Dis., v.9, n.9, 16p, Sept. 2015.
1935-2727
10.1371/journal.pntd.0004064
1935-2735
url https://www.arca.fiocruz.br/handle/icict/14440
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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