Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50827 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
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Ferreira, Aline TátilaGarcia, Gabriela A.Santos, Lilha M. B. dosPavan, Márcio G.Moreira, Carlos José de C.Victoriano, Juliana C.Silva Junior, Renato daSantos-Mallet, Jacenir R. dosVerly, ThaianeBrito, ConstançaSikulu‑Lord, Maggy T.Freitas, Rafael Maciel de2022-01-20T13:58:12Z2022-01-20T13:58:12Z2021FERREIRA, Aline Tátila et al. Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans. Scientific Reports, v. 11, 23884, p. 1 - 10, 2021.2045-2322https://www.arca.fiocruz.br/handle/icict/5082710.1038/s41598-021-03465-8engNatureTriatoma infestansEspectroscopia de infravermelhoDetecçãoAmostras de reto e excretasIntestino médioTrypanosoma cruziNear infrared spectroscopyAccurately detectsTrypanosoma cruzinon‑destructively in midgutsrectum and excreta samplesTriatoma infestansNear infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Nova Iguaçu, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Nova Iguaçu, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.The School of Public Health, The University of Queensland. Herston, QLD 4006, Australia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi parasite with an estimated 70 million people at risk. Traditionally, parasite presence in triatomine vectors is detected through optical microscopy which can be low in sensitivity or molecular techniques which can be costly in endemic countries. The aim of this study was to evaluate the ability of a reagent-free technique, the Near Infrared Spectroscopy (NIRS) for rapid and non-invasive detection of T. cruzi in Triatoma infestans body parts and in wet/dry excreta samples of the insect. NIRS was 100% accurate for predicting the presence of T. cruzi infection Dm28c strain (TcI) in either the midgut or the rectum and models developed from either body part could predict infection in the other part. Models developed to predict infection in excreta samples were 100% accurate for predicting infection in both wet and dry samples. However, models developed using dry excreta could not predict infection in wet samples and vice versa. This is the first study to report on the potential application of NIRS for rapid and non-invasive detection of T. cruzi infection in T. infestans in the laboratory. Future work should demonstrate the capacity of NIRS to detect T. cruzi in triatomines originating from the field.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50827/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJacenirMallet_ConstançaBrito_etal_IOC_2021.pdfJacenirMallet_ConstançaBrito_etal_IOC_2021.pdfapplication/pdf3074803https://www.arca.fiocruz.br/bitstream/icict/50827/2/JacenirMallet_Constan%c3%a7aBrito_etal_IOC_2021.pdf5d3b3bc4abacac5fa81e0fb8d8801274MD52icict/508272022-01-20 10:58:12.037oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-01-20T13:58:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| title |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| spellingShingle |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans Ferreira, Aline Tátila Triatoma infestans Espectroscopia de infravermelho Detecção Amostras de reto e excretas Intestino médio Trypanosoma cruzi Near infrared spectroscopy Accurately detects Trypanosoma cruzi non‑destructively in midguts rectum and excreta samples Triatoma infestans |
| title_short |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| title_full |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| title_fullStr |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| title_full_unstemmed |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| title_sort |
Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans |
| author |
Ferreira, Aline Tátila |
| author_facet |
Ferreira, Aline Tátila Garcia, Gabriela A. Santos, Lilha M. B. dos Pavan, Márcio G. Moreira, Carlos José de C. Victoriano, Juliana C. Silva Junior, Renato da Santos-Mallet, Jacenir R. dos Verly, Thaiane Brito, Constança Sikulu‑Lord, Maggy T. Freitas, Rafael Maciel de |
| author_role |
author |
| author2 |
Garcia, Gabriela A. Santos, Lilha M. B. dos Pavan, Márcio G. Moreira, Carlos José de C. Victoriano, Juliana C. Silva Junior, Renato da Santos-Mallet, Jacenir R. dos Verly, Thaiane Brito, Constança Sikulu‑Lord, Maggy T. Freitas, Rafael Maciel de |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Aline Tátila Garcia, Gabriela A. Santos, Lilha M. B. dos Pavan, Márcio G. Moreira, Carlos José de C. Victoriano, Juliana C. Silva Junior, Renato da Santos-Mallet, Jacenir R. dos Verly, Thaiane Brito, Constança Sikulu‑Lord, Maggy T. Freitas, Rafael Maciel de |
| dc.subject.other.pt_BR.fl_str_mv |
Triatoma infestans Espectroscopia de infravermelho Detecção Amostras de reto e excretas Intestino médio Trypanosoma cruzi |
| topic |
Triatoma infestans Espectroscopia de infravermelho Detecção Amostras de reto e excretas Intestino médio Trypanosoma cruzi Near infrared spectroscopy Accurately detects Trypanosoma cruzi non‑destructively in midguts rectum and excreta samples Triatoma infestans |
| dc.subject.en.pt_BR.fl_str_mv |
Near infrared spectroscopy Accurately detects Trypanosoma cruzi non‑destructively in midguts rectum and excreta samples Triatoma infestans |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021 |
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2022-01-20T13:58:12Z |
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2022-01-20T13:58:12Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FERREIRA, Aline Tátila et al. Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans. Scientific Reports, v. 11, 23884, p. 1 - 10, 2021. |
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https://www.arca.fiocruz.br/handle/icict/50827 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-021-03465-8 |
| identifier_str_mv |
FERREIRA, Aline Tátila et al. Near infrared spectroscopy accurately detects Trypanosoma cruzi non‑destructively in midguts, rectum and excreta samples of Triatoma infestans. Scientific Reports, v. 11, 23884, p. 1 - 10, 2021. 2045-2322 10.1038/s41598-021-03465-8 |
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Nature |
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Nature |
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