COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59088 https://doi.org/10.1016/j.mtchem.2023.101597 |
Resumo: | Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Laboratório de Células Inflamatórias e Neoplásicas. Curitiba, PR, Brasil. |
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Santos, A.Brandão, A. P. Macedo de SouzaHryniewicz, B. M.Abreu, H.Bach-Toledo, L.Silva, S. Schuster daDeller, A. E.Rogerio, V. Z.Rodrigues, D. S. BaêtaHiraiwa, Priscila MazzocchiGuimarães, Beatriz GomesMarchesi, L. F.Oliveira, J. Carvalho deGradia, D. F.Soares, F. L. F.Zanchin, Nilson Ivo ToninOliveira, C. Camargo deVidotti, M.2023-06-20T20:20:46Z2023-06-20T20:20:46Z2023SANTOS, A. et al. COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples. Materials TodayChemistry, v. 30, n. 101597, p. 1-18, 2023.2468-5194https://www.arca.fiocruz.br/handle/icict/59088https://doi.org/10.1016/j.mtchem.2023.101597porElsevierCOVID-19Biossensor impedimétricoBiosensing TechniquesImpedimetric biosensorNanostructuresElectrodesChemometricsTécnicas BiosensiblesNanoestructurasElectrodosQuimiometríaTechniques de biocapteurNanostructuresÉlectrodesChimiométrieTécnicas BiossensoriaisNanoestruturasEletrodosQuimiometriaSalivaCOVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Laboratório de Células Inflamatórias e Neoplásicas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Grupo de Pesquisa em Macromoléculas e Interfaces. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Laboratório de Citogenética Humana e Oncogenética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Grupo de Pesquisa em Macromoléculas e Interfaces. Curitiba, PR, Brasil. / Centro de Tecnologia da Informação Renato Archer. Campinas, SP, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Laboratório de Células Inflamatórias e Neoplásicas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Grupo de Pesquisa em Macromoléculas e Interfaces. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Tecnológica Federal do Paraná. Grupo de Estudos em Espectroscopia de Impedância Eletroquímica. Ponta Grossa, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Laboratório de Citogenética Humana e Oncogenética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Programa de Pós-Graduação em Genética. Laboratório de Citogenética Humana e Oncogenética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Grupo de Pesquisa em Macromoléculas e Interfaces. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Laboratório de Células Inflamatórias e Neoplásicas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Grupo de Pesquisa em Macromoléculas e Interfaces. Curitiba, PR, Brasil.SARS-CoV-2 rapid spread required urgent, accurate, and prompt diagnosis to control the vírus dissemination and pandemic management. Several sensors were developed using diferente biorecognition elements to obtain high specificity and sensitivity. However, the task to achieve these parameters in combination with fast detection, simplicity, and portability to identify the biorecognition element even in low concentration remains a challenge. Therefore, we developed an electrochemical biosensor based on polypyrrole nanotubes coupled via Ni(OH)2 ligation to an engineered antigen-binding fragmented of heavy chain-only antibodies (VHH) termed Sb#15. Here in were port Sb#15-His6 expression, purification, and characterization of its interaction with the receptor-binding domain (RBD) of SARS-CoV-2 in addition to the construction and validation of a biosensor.There combinant Sb#15 is correctly folded and interacts with the RBD with a dissociation constant( KD) of 27.1 ± 6.4 nmol/ L. The biosensing platform was developed using polypyrrole nanotubes and Ni(OH)2, which can properly orientate the immobilization of Sb#15-His6 at the electrode surface through His-tag interaction for the sensitive SARS-CoV-2 antigen detection. The quantification limit was determined as 0.01pg /mL using recombinant RBD, which was expressively lower than comercial monoclonal antibodies. Inpre-characterized saliva, both Omicron and Delta SARS-CoV-2 were accurately detected Only in positive samples, meeting all the requirements recommended by the World Health Organization for in vitro diagnostics. Allow sample volume of saliva is needed to perform the detection, providing results within 15 min without further sample preparations. In summary, new perspective allying recombinant VHHs with biosensor development and real sample detection was explored, addressing the need for accurate, rapid, and sensitive biosensors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCOVID-19ImpedimetricOK.pdfCOVID-19ImpedimetricOK.pdfapplication/pdf3101966https://www.arca.fiocruz.br/bitstream/icict/59088/2/COVID-19ImpedimetricOK.pdf022801201420fe5b3211f4b4144346feMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59088/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/590882023-06-20 17:20:48.297oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-20T20:20:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| title |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| spellingShingle |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples Santos, A. COVID-19 Biossensor impedimétrico Biosensing Techniques Impedimetric biosensor Nanostructures Electrodes Chemometrics Técnicas Biosensibles Nanoestructuras Electrodos Quimiometría Techniques de biocapteur Nanostructures Électrodes Chimiométrie Técnicas Biossensoriais Nanoestruturas Eletrodos Quimiometria Saliva |
| title_short |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| title_full |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| title_fullStr |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| title_full_unstemmed |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| title_sort |
COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples |
| author |
Santos, A. |
| author_facet |
Santos, A. Brandão, A. P. Macedo de Souza Hryniewicz, B. M. Abreu, H. Bach-Toledo, L. Silva, S. Schuster da Deller, A. E. Rogerio, V. Z. Rodrigues, D. S. Baêta Hiraiwa, Priscila Mazzocchi Guimarães, Beatriz Gomes Marchesi, L. F. Oliveira, J. Carvalho de Gradia, D. F. Soares, F. L. F. Zanchin, Nilson Ivo Tonin Oliveira, C. Camargo de Vidotti, M. |
| author_role |
author |
| author2 |
Brandão, A. P. Macedo de Souza Hryniewicz, B. M. Abreu, H. Bach-Toledo, L. Silva, S. Schuster da Deller, A. E. Rogerio, V. Z. Rodrigues, D. S. Baêta Hiraiwa, Priscila Mazzocchi Guimarães, Beatriz Gomes Marchesi, L. F. Oliveira, J. Carvalho de Gradia, D. F. Soares, F. L. F. Zanchin, Nilson Ivo Tonin Oliveira, C. Camargo de Vidotti, M. |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, A. Brandão, A. P. Macedo de Souza Hryniewicz, B. M. Abreu, H. Bach-Toledo, L. Silva, S. Schuster da Deller, A. E. Rogerio, V. Z. Rodrigues, D. S. Baêta Hiraiwa, Priscila Mazzocchi Guimarães, Beatriz Gomes Marchesi, L. F. Oliveira, J. Carvalho de Gradia, D. F. Soares, F. L. F. Zanchin, Nilson Ivo Tonin Oliveira, C. Camargo de Vidotti, M. |
| dc.subject.other.en_US.fl_str_mv |
COVID-19 Biossensor impedimétrico |
| topic |
COVID-19 Biossensor impedimétrico Biosensing Techniques Impedimetric biosensor Nanostructures Electrodes Chemometrics Técnicas Biosensibles Nanoestructuras Electrodos Quimiometría Techniques de biocapteur Nanostructures Électrodes Chimiométrie Técnicas Biossensoriais Nanoestruturas Eletrodos Quimiometria Saliva |
| dc.subject.en.en_US.fl_str_mv |
Biosensing Techniques Impedimetric biosensor Nanostructures Electrodes Chemometrics |
| dc.subject.es.en_US.fl_str_mv |
Técnicas Biosensibles Nanoestructuras Electrodos Quimiometría |
| dc.subject.fr.en_US.fl_str_mv |
Techniques de biocapteur Nanostructures Électrodes Chimiométrie |
| dc.subject.decs.en_US.fl_str_mv |
Técnicas Biossensoriais Nanoestruturas Eletrodos Quimiometria Saliva |
| description |
Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Biologia Celular. Laboratório de Células Inflamatórias e Neoplásicas. Curitiba, PR, Brasil. |
| publishDate |
2023 |
| dc.date.accessioned.fl_str_mv |
2023-06-20T20:20:46Z |
| dc.date.available.fl_str_mv |
2023-06-20T20:20:46Z |
| dc.date.issued.fl_str_mv |
2023 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SANTOS, A. et al. COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples. Materials TodayChemistry, v. 30, n. 101597, p. 1-18, 2023. |
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https://www.arca.fiocruz.br/handle/icict/59088 |
| dc.identifier.issn.en_US.fl_str_mv |
2468-5194 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.mtchem.2023.101597 |
| identifier_str_mv |
SANTOS, A. et al. COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples. Materials TodayChemistry, v. 30, n. 101597, p. 1-18, 2023. 2468-5194 |
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https://www.arca.fiocruz.br/handle/icict/59088 https://doi.org/10.1016/j.mtchem.2023.101597 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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