A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers

Detalhes bibliográficos
Autor(a) principal: Morawski, Franciele de Matos
Data de Publicação: 2023
Outros Autores: Martins, Gustavo, Ramos, Maria Karolina, Zarbin, Aldo J.G., Blanes, Lucas, Bergamini, Marcio F., Marcolino-Junior, Luiz Humberto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58630
https://doi.org/10.1016/j.aca.2023.341169
Resumo: Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.
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spelling Morawski, Franciele de MatosMartins, GustavoRamos, Maria KarolinaZarbin, Aldo J.G.Blanes, LucasBergamini, Marcio F.Marcolino-Junior, Luiz Humberto2023-05-24T18:09:18Z2023-05-24T18:09:18Z2023MORAWSKI, Franciele de Matos et al. A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers. Analytica Chimica Acta, v. 1258, p. 1-9, 2023.0003-2670https://www.arca.fiocruz.br/handle/icict/58630https://doi.org/10.1016/j.aca.2023.341169porElsevierImpressão TridimensionalPrinting, Three-DimensionalFused Deposition ModellingMultiplex Voltammetric DetectionBiosensing TechniquesImpresión TridimensionalTécnicas BiosensiblesImpression tridimensionnelleTechniques de biocapteurImpressão em 3DSARS-CoV-2Técnicas BiossensoriaisA versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Grupo de Química de Materiais. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Grupo de Química de Materiais. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.3D-printing has shown an outstanding performance for the production of versatile electrochemical devices. However, there is a lack of studies in the field of 3D-printed miniaturized settings for multiplex biosensing. In this work, we propose a fully 3D-printed micro-volume cell containing six working electrodes (WEs) that operates with 250 μL of sample. A polylactic acid/carbon black conductive filament (PLA/CB) was used to print the WEs and subsequently modified with graphene oxide (GO), to support protein binding. Cyclic voltammetry was employed to investigate the electrochemical behaviour of the novel multi-electrode cell. In the presence of K₃[Fe (CN)₆], PLA/CB/GO showed adequate peak resolution for subsequent label-free immunosensing. The innovative 3D-printed cell was applied for multiplex voltammetric detection of three COVID-19 biomarkers as a proof-of concept. The multiple sensors showed a wide linear range with detection limits of 5, 1 and 1 pg mL− 1 for Nprotein, SRBD-protein, and anti-SRBD, respectively. The sensor performance enabled the selective sequential detection of N protein, SRBD protein, and anti-SRBD at biological levels in saliva and serum. In summary, the miniaturized six-electrode cell presents an alternative for the low-cost and fast production of customizable devices for multi-target sensing with promising application in the development of point-of-care sensors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58630/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL1-s2.0-S0003267023003902-mainOK.pdf1-s2.0-S0003267023003902-mainOK.pdfapplication/pdf4599377https://www.arca.fiocruz.br/bitstream/icict/58630/2/1-s2.0-S0003267023003902-mainOK.pdf9f978f88bbdd256df6fe2d6dd4ff1c4fMD52icict/586302023-05-24 15:09:20.146oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-24T18:09:20Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
title A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
spellingShingle A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
Morawski, Franciele de Matos
Impressão Tridimensional
Printing, Three-Dimensional
Fused Deposition Modelling
Multiplex Voltammetric Detection
Biosensing Techniques
Impresión Tridimensional
Técnicas Biosensibles
Impression tridimensionnelle
Techniques de biocapteur
Impressão em 3D
SARS-CoV-2
Técnicas Biossensoriais
title_short A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
title_full A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
title_fullStr A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
title_full_unstemmed A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
title_sort A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers
author Morawski, Franciele de Matos
author_facet Morawski, Franciele de Matos
Martins, Gustavo
Ramos, Maria Karolina
Zarbin, Aldo J.G.
Blanes, Lucas
Bergamini, Marcio F.
Marcolino-Junior, Luiz Humberto
author_role author
author2 Martins, Gustavo
Ramos, Maria Karolina
Zarbin, Aldo J.G.
Blanes, Lucas
Bergamini, Marcio F.
Marcolino-Junior, Luiz Humberto
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morawski, Franciele de Matos
Martins, Gustavo
Ramos, Maria Karolina
Zarbin, Aldo J.G.
Blanes, Lucas
Bergamini, Marcio F.
Marcolino-Junior, Luiz Humberto
dc.subject.other.en_US.fl_str_mv Impressão Tridimensional
topic Impressão Tridimensional
Printing, Three-Dimensional
Fused Deposition Modelling
Multiplex Voltammetric Detection
Biosensing Techniques
Impresión Tridimensional
Técnicas Biosensibles
Impression tridimensionnelle
Techniques de biocapteur
Impressão em 3D
SARS-CoV-2
Técnicas Biossensoriais
dc.subject.en.en_US.fl_str_mv Printing, Three-Dimensional
Fused Deposition Modelling
Multiplex Voltammetric Detection
Biosensing Techniques
dc.subject.es.en_US.fl_str_mv Impresión Tridimensional
Técnicas Biosensibles
dc.subject.fr.en_US.fl_str_mv Impression tridimensionnelle
Techniques de biocapteur
dc.subject.decs.en_US.fl_str_mv Impressão em 3D
SARS-CoV-2
Técnicas Biossensoriais
description Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-05-24T18:09:18Z
dc.date.available.fl_str_mv 2023-05-24T18:09:18Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAWSKI, Franciele de Matos et al. A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers. Analytica Chimica Acta, v. 1258, p. 1-9, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58630
dc.identifier.issn.en_US.fl_str_mv 0003-2670
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.aca.2023.341169
identifier_str_mv MORAWSKI, Franciele de Matos et al. A versatile 3D printed multi-electrode cell for determination of three COVID-19 biomarkers. Analytica Chimica Acta, v. 1258, p. 1-9, 2023.
0003-2670
url https://www.arca.fiocruz.br/handle/icict/58630
https://doi.org/10.1016/j.aca.2023.341169
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58630/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/58630/2/1-s2.0-S0003267023003902-mainOK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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