Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33462 |
Resumo: | O artigo encontra-se disponível para download no site do Editor. |
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Picelli, Carina G.Borges, Rafael J.Fernandes, Carlos A. H.Matioli, Fabio M.Fernandes, Carla Freire CeledônioSobrinho, Juliana C.Holanda, Rudson JesusOzaki, Luiz S.Kayano, Anderson MakotoCalderon, Leonardo de AzevedoFontes, Marcos R. M.Stábeli, Rodrigo GuerinoSoares, Andreimar Martins2019-06-11T19:58:11Z2019-06-11T19:58:11Z2017PICELLI, Carina G. et al. Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes. International Journal of Biological Macromolecules, v. 103, p. 525-532, 2017.0141-8130https://www.arca.fiocruz.br/handle/icict/3346210.1016/j.ijbiomac.2017.05.0761879-0003O artigo encontra-se disponível para download no site do Editor.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil / Virginia Commonwealth University. Center for the Study of Biological Complexity, Life Sciences. Richmond, USA.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Universidade Estadual Paulista. Instituto de Biociências. Departamento de Física e Biofísica. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil. / Centro Universitário São Lucas. Porto Velho, RO, Brasil.Phospholipases A2 inhibitors (PLIs) produced by venomous and non-venomous snakαes play essential role in this resistance. These endogenous inhibitors may be classified by their fold in PLIα, PLIβ and PLIγ. Phospholipases A2 (PLA2s) develop myonecrosis in snake envenomation, a consequence that is not efficiently neutralized by antivenom treatment. This work aimed to identify and characterize two PLIs from Amazonian snake species, Bothrops atrox and Micrurus lemniscatus. Liver tissues RNA of specimens from each species were isolated and amplified by RT-PCR using PCR primers based on known PLIγ gene sequences, followed by cloning and sequencing of amplified fragments. Sequence similarity studies showed elevated identity with inhibitor PLIγ gene sequences from other snake species. Molecular models of translated inhibitors’ gene sequences resemble canonical three finger fold from PLIγ and support the hypothesis that the decapeptide (residues 107–116) may be responsible for PLA2 inhibition. Structural studies and action mechanism of these PLIs may provide necessary information to evaluate their potential as antivenom or as complement of the current ophidian accident treatment.engElsevierMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSnakesBothrops atroxMicrurus lemniscatusInhibitors of phospholipases A2Gamma type phospholipase A2 inhibitorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33462/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes.pdfMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes.pdfapplication/pdf1752222https://www.arca.fiocruz.br/bitstream/icict/33462/2/Molecular%20cloning%20and%20structural%20modelling%20of%20gamma-phospholipase%20A2%20inhibitors%20from%20Bothrops%20atrox%20and%20Micrurus%20lemniscatus%20snakes.pdf310894a14a1b6c8b7285e668e031e864MD52TEXTMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes.pdf.txtMolecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes.pdf.txtExtracted texttext/plain45872https://www.arca.fiocruz.br/bitstream/icict/33462/3/Molecular%20cloning%20and%20structural%20modelling%20of%20gamma-phospholipase%20A2%20inhibitors%20from%20Bothrops%20atrox%20and%20Micrurus%20lemniscatus%20snakes.pdf.txtd62391ad68619d605489d9971aadcb4dMD53icict/334622019-06-15 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| dc.title.pt_BR.fl_str_mv |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| title |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| spellingShingle |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes Picelli, Carina G. Snakes Bothrops atrox Micrurus lemniscatus Inhibitors of phospholipases A2 Gamma type phospholipase A2 inhibitors |
| title_short |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| title_full |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| title_fullStr |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| title_full_unstemmed |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| title_sort |
Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes |
| author |
Picelli, Carina G. |
| author_facet |
Picelli, Carina G. Borges, Rafael J. Fernandes, Carlos A. H. Matioli, Fabio M. Fernandes, Carla Freire Celedônio Sobrinho, Juliana C. Holanda, Rudson Jesus Ozaki, Luiz S. Kayano, Anderson Makoto Calderon, Leonardo de Azevedo Fontes, Marcos R. M. Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| author_role |
author |
| author2 |
Borges, Rafael J. Fernandes, Carlos A. H. Matioli, Fabio M. Fernandes, Carla Freire Celedônio Sobrinho, Juliana C. Holanda, Rudson Jesus Ozaki, Luiz S. Kayano, Anderson Makoto Calderon, Leonardo de Azevedo Fontes, Marcos R. M. Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Picelli, Carina G. Borges, Rafael J. Fernandes, Carlos A. H. Matioli, Fabio M. Fernandes, Carla Freire Celedônio Sobrinho, Juliana C. Holanda, Rudson Jesus Ozaki, Luiz S. Kayano, Anderson Makoto Calderon, Leonardo de Azevedo Fontes, Marcos R. M. Stábeli, Rodrigo Guerino Soares, Andreimar Martins |
| dc.subject.en.pt_BR.fl_str_mv |
Snakes Bothrops atrox Micrurus lemniscatus Inhibitors of phospholipases A2 Gamma type phospholipase A2 inhibitors |
| topic |
Snakes Bothrops atrox Micrurus lemniscatus Inhibitors of phospholipases A2 Gamma type phospholipase A2 inhibitors |
| description |
O artigo encontra-se disponível para download no site do Editor. |
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2017 |
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2017 |
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2019-06-11T19:58:11Z |
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2019-06-11T19:58:11Z |
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PICELLI, Carina G. et al. Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes. International Journal of Biological Macromolecules, v. 103, p. 525-532, 2017. |
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https://www.arca.fiocruz.br/handle/icict/33462 |
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0141-8130 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.ijbiomac.2017.05.076 |
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1879-0003 |
| identifier_str_mv |
PICELLI, Carina G. et al. Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes. International Journal of Biological Macromolecules, v. 103, p. 525-532, 2017. 0141-8130 10.1016/j.ijbiomac.2017.05.076 1879-0003 |
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Elsevier |
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Elsevier |
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