Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45723 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil. |
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Ramos, Tadeu DinizSilva, Johnatas DutraMartins, Alessandra Marcia da FonsecaPratti, Juliana Elena da SilveiraCruz, Luan FirminoOliveira, Diogo MacielSantos, Julio Souza dosTenorio, João Ivo NunesAraujo, Almair Ferreira deLima, Célio Geraldo Freire deDiaz, Bruno LourençoCrruz, Fernanda FerreiraRocco, Patricia Rieken MacedoGuedes, Herbert Leonel de Matos2021-01-16T14:05:21Z2021-01-16T14:05:21Z2020RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020.2637-9589https://www.arca.fiocruz.br/handle/icict/4572310.1186/s13287-020-01889-zengBMCLeishmanioseCélulas estromais mesenquimaisCélulas mesenquimal derivadas do tecido adiposoAntimoniato de megluminaCélulas mesenquimais mesenquimais derivadas da medula ósseaCarga de parasitasLeishmaniasisMesenchymal stromal cellsAdipose tissue-derived mesenchymal stromal cellsMeglumine antimoniateBone marrow-derived mesenchymal stromal cellsParasite loadCombined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Medicina Regeneratica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Campus Duque de Caxias Professor Geraldo Cidade. Duque de Caxias, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Background: Leishmaniasis is a neglected disease caused by Leishmania spp. One of its characteristics is an imbalance of host immune responses to foster parasite survival. In this setting, mesenchymal stromal cells (MSCs) may be a viable therapeutic alternative, given their well-established immunomodulatory potential. In this study, we compared the effects of therapy with bone marrow (BM)- and adipose tissue (AD)-derived MSCs in leishmaniasis caused by Leishmania amazonensis in C57BL/6 mice. After determining the most effective MSC source, we then combined these cells with meglumine antimoniate (a pentavalent antimonial commonly used for the treatment of leishmaniasis) to treat the infected mice. Methods: In vitro, co-culture of AD-MSCs and BM-MSCs with Leishmania amazonensis-infected macrophages was performed to understand the influence of both MSC sources in infected cells. In vivo, infected C57BL/6 mice were treated with phosphate-buffered saline (PBS), AD-MSCs and BM-MSCs, and then meglumine antimoniate was combined with MSCs from the most effective source.Results: In vitro, co-culture of Leishmania amazonensis-infected macrophages with BM-MSCs, compared to ADMSCs, led to a higher parasite load and lower production of nitric oxide. Fibroblasts grown in conditioned medium from co-cultures with AD-MSCs promoted faster wound healing. Despite a non-significant difference in the production of vascular endothelial growth factor, we observed higher production of tumor necrosis factor-α and interleukin (IL)-10 in the co-culture with AD-MSCs. In vivo, treatment of infected mice with BM-MSCs did not lead to disease control; however, the use of AD-MSCs was associated with partial control of lesion development, without significant differences in the parasite load. AD-MSCs combined with meglumine antimoniate reduced lesion size and parasite load when compared to PBS and AD-MSC groups. At the infection site, we detected a small production of IL-10, but we were unable to detect production of either IL-4 or interferon-γ, indicating resolution of infection without effect on the percentage of regulatory T cells. Conclusion: Combination treatment of cutaneous leishmaniasis with AD-MSCs and meglumine antimoniate may be a viable alternative.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45723/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHerbetLM_Guedes_etal_IOC_2020.pdfHerbetLM_Guedes_etal_IOC_2020.pdfapplication/pdf1535937https://www.arca.fiocruz.br/bitstream/icict/45723/2/HerbetLM_Guedes_etal_IOC_2020.pdf4a0a23eb981d92fbb0552f35aab8b4f3MD52TEXTHerbetLM_Guedes_etal_IOC_2020.pdf.txtHerbetLM_Guedes_etal_IOC_2020.pdf.txtExtracted texttext/plain74526https://www.arca.fiocruz.br/bitstream/icict/45723/3/HerbetLM_Guedes_etal_IOC_2020.pdf.txtf88dd660984b18c30bed52a312271444MD53icict/457232021-01-18 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| dc.title.pt_BR.fl_str_mv |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| title |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| spellingShingle |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis Ramos, Tadeu Diniz Leishmaniose Células estromais mesenquimais Células mesenquimal derivadas do tecido adiposo Antimoniato de meglumina Células mesenquimais mesenquimais derivadas da medula óssea Carga de parasitas Leishmaniasis Mesenchymal stromal cells Adipose tissue-derived mesenchymal stromal cells Meglumine antimoniate Bone marrow-derived mesenchymal stromal cells Parasite load |
| title_short |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| title_full |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| title_fullStr |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| title_full_unstemmed |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| title_sort |
Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis |
| author |
Ramos, Tadeu Diniz |
| author_facet |
Ramos, Tadeu Diniz Silva, Johnatas Dutra Martins, Alessandra Marcia da Fonseca Pratti, Juliana Elena da Silveira Cruz, Luan Firmino Oliveira, Diogo Maciel Santos, Julio Souza dos Tenorio, João Ivo Nunes Araujo, Almair Ferreira de Lima, Célio Geraldo Freire de Diaz, Bruno Lourenço Crruz, Fernanda Ferreira Rocco, Patricia Rieken Macedo Guedes, Herbert Leonel de Matos |
| author_role |
author |
| author2 |
Silva, Johnatas Dutra Martins, Alessandra Marcia da Fonseca Pratti, Juliana Elena da Silveira Cruz, Luan Firmino Oliveira, Diogo Maciel Santos, Julio Souza dos Tenorio, João Ivo Nunes Araujo, Almair Ferreira de Lima, Célio Geraldo Freire de Diaz, Bruno Lourenço Crruz, Fernanda Ferreira Rocco, Patricia Rieken Macedo Guedes, Herbert Leonel de Matos |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ramos, Tadeu Diniz Silva, Johnatas Dutra Martins, Alessandra Marcia da Fonseca Pratti, Juliana Elena da Silveira Cruz, Luan Firmino Oliveira, Diogo Maciel Santos, Julio Souza dos Tenorio, João Ivo Nunes Araujo, Almair Ferreira de Lima, Célio Geraldo Freire de Diaz, Bruno Lourenço Crruz, Fernanda Ferreira Rocco, Patricia Rieken Macedo Guedes, Herbert Leonel de Matos |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Células estromais mesenquimais Células mesenquimal derivadas do tecido adiposo Antimoniato de meglumina Células mesenquimais mesenquimais derivadas da medula óssea Carga de parasitas |
| topic |
Leishmaniose Células estromais mesenquimais Células mesenquimal derivadas do tecido adiposo Antimoniato de meglumina Células mesenquimais mesenquimais derivadas da medula óssea Carga de parasitas Leishmaniasis Mesenchymal stromal cells Adipose tissue-derived mesenchymal stromal cells Meglumine antimoniate Bone marrow-derived mesenchymal stromal cells Parasite load |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Mesenchymal stromal cells Adipose tissue-derived mesenchymal stromal cells Meglumine antimoniate Bone marrow-derived mesenchymal stromal cells Parasite load |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Grupo de Imunologia e Vacinologia,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020 |
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2021-01-16T14:05:21Z |
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2021-01-16T14:05:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020. |
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https://www.arca.fiocruz.br/handle/icict/45723 |
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2637-9589 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13287-020-01889-z |
| identifier_str_mv |
RAMOS, Tadeu Diniz et al. Combined therapy with adipose tissuederived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis. Stem Cell Research & Therapy, v. 11, n. 374, p. 1-15, 2020. 2637-9589 10.1186/s13287-020-01889-z |
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https://www.arca.fiocruz.br/handle/icict/45723 |
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