Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum

Detalhes bibliográficos
Autor(a) principal: Lima, Isadora dos Santos
Data de Publicação: 2019
Outros Autores: Solcá, Manuela da Silva, Tafuri, Wagner Luiz, Freitas, Luiz Antonio Rodrigues de, Santos, Washington Luis Conrado dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39312
Resumo: Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005
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spelling Lima, Isadora dos SantosSolcá, Manuela da SilvaTafuri, Wagner LuizFreitas, Luiz Antonio Rodrigues deSantos, Washington Luis Conrado dos2020-01-20T12:27:29Z2020-01-20T12:27:29Z2019LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3931210.1186/s13071-019-3723-1Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005Grant No. DTE0037/2011Oswaldo Cruz Foundation (Fiocruz; http://portal.fiocruz.br/pt-br) PAPES V, grant 403470/2008-0Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Background: The liver plays a central role in the development of canine visceral leishmaniasis. Studies of natural infection in animals and humans indicate a direct relationship between resolution of infection and the formation and maturation of granulomas in the liver. However, in contrast to other reports in the literature, the present study found no diferences in the characteristics of hepatic granulomas that could be related to resistance or susceptibility to Leishmania. Here, we describe the hepatic alterations observed in dogs with difering clinical manifestations of visceral leishmaniasis in an endemic area in the state of Bahia, Brazil. Methods: We examined 148 animals in an endemic area. The animals were clinically examined, and the infection was determined by ELISA, spleen aspirate culture and quantitative PCR. The animals were grouped into asymptomatic or symptomatic based on the number of signs of LV. The histological liver evaluation was performed in a blinded way. Results: Our results indicated no association between the characteristics of granulomas and clinical presentation. We found an association between the intensity of this infammatory response and parasite load in the animals’ spleens. It is important to note that while hepatic alterations, such as portal and perivascular infammation and the presence of larger amounts of granulomas, were linked with higher parasite loads, we found the inverse to be true with respect to intrasinusoidal lymphocytosis, the formation of intrasinusoidal infammatory cell aggregates and Kupfer cell hypertrophy. Conclusions: Our fndings suggest that the presence of mononuclear infammatory cells inside the sinusoids is more important than that of organized granulomas in terms of the containment of parasitism by the host. We suggest that the presence of granulomas indicates the failure of a frst line of defense mechanism in the control of parasite infection, which could be related to the presence of infammatory cells and Kupfer cell hypertrophy inside the sinusoids. We further demonstrated that dogs with active Leishmania spp. infection present a higher frequency of infammatory changes in the liver. In addition to being correlated with the severity of clinical manifestation, these hepatic alterations were also associated with changes in hematological and biochemical parameters.engBMCLeishmania infantumLeishmaniose visceral caninaHistopatologia hepáticaPatologiaInflamaçãoGranulomaLeishmania infantumCanine visceral leishmaniasisLiver histopathologyPathologyInfammationGranulomaAssessment of histological liver alterations in dogs naturally infected with Leishmania infantuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39312/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLima I S Assessment of ...Parasites & Vectors.pdfLima I S Assessment of ...Parasites & Vectors.pdfapplication/pdf2867832https://www.arca.fiocruz.br/bitstream/icict/39312/2/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf4f6943af6c3a941b6399108be47ed653MD52TEXTLima I S Assessment of ...Parasites & Vectors.pdf.txtLima I S Assessment of ...Parasites & Vectors.pdf.txtExtracted texttext/plain52748https://www.arca.fiocruz.br/bitstream/icict/39312/3/Lima%20I%20S%20Assessment%20of%20...Parasites%20%26%20Vectors.pdf.txt4f21d3c17f278dfbbf2d4f61b2c971b0MD53icict/393122023-03-15 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dc.title.en.fl_str_mv Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
title Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
spellingShingle Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
Lima, Isadora dos Santos
Leishmania infantum
Leishmaniose visceral canina
Histopatologia hepática
Patologia
Inflamação
Granuloma
Leishmania infantum
Canine visceral leishmaniasis
Liver histopathology
Pathology
Infammation
Granuloma
title_short Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
title_full Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
title_fullStr Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
title_full_unstemmed Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
title_sort Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum
author Lima, Isadora dos Santos
author_facet Lima, Isadora dos Santos
Solcá, Manuela da Silva
Tafuri, Wagner Luiz
Freitas, Luiz Antonio Rodrigues de
Santos, Washington Luis Conrado dos
author_role author
author2 Solcá, Manuela da Silva
Tafuri, Wagner Luiz
Freitas, Luiz Antonio Rodrigues de
Santos, Washington Luis Conrado dos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima, Isadora dos Santos
Solcá, Manuela da Silva
Tafuri, Wagner Luiz
Freitas, Luiz Antonio Rodrigues de
Santos, Washington Luis Conrado dos
dc.subject.other.pt_BR.fl_str_mv Leishmania infantum
Leishmaniose visceral canina
Histopatologia hepática
Patologia
Inflamação
Granuloma
topic Leishmania infantum
Leishmaniose visceral canina
Histopatologia hepática
Patologia
Inflamação
Granuloma
Leishmania infantum
Canine visceral leishmaniasis
Liver histopathology
Pathology
Infammation
Granuloma
dc.subject.en.en.fl_str_mv Leishmania infantum
Canine visceral leishmaniasis
Liver histopathology
Pathology
Infammation
Granuloma
description Fundação de Amparo à Pesquisa do Estado da Bahia (Fapesb. http://www.fapes b.ba.gov.br) Grant No. APR0434/2005
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-01-20T12:27:29Z
dc.date.available.fl_str_mv 2020-01-20T12:27:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39312
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s13071-019-3723-1
identifier_str_mv LIMA, Isadora dos Santos et al. Assessment of histological liver alterations in dogs naturally infected with Leishmania infantum. Parasites and Vectors, v. 12, n. 487, p. 1-14, 16 Oct. 2019.
1756-3305
10.1186/s13071-019-3723-1
url https://www.arca.fiocruz.br/handle/icict/39312
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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