Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.

Detalhes bibliográficos
Autor(a) principal: Nicolato, Roney Luiz de Carvalho
Data de Publicação: 2013
Outros Autores: Abreu, Raquel Trópia de, Roatt, Bruno Mendes, Soares, Rodrigo Dian de Oliveira Aguiar, Reis, Levi Eduardo Soares, Carvalho, Maria das Graças, Carneiro, Cláudia Martins, Giunchetti, Rodolfo Cordeiro, Bouillet, Leoneide Érica Maduro, Lemos, Denise da Silveira, Vital, Wendel Coura, Reis, Alexandre Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4313
https://doi.org/10.1371/journal.pone.0082947
Resumo: Hematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density in dogs naturally infected by L. infantum. To further characterize these alterations, we evaluated the association between the hematological parameters in bone marrow and peripheral blood alterations in groups of L. infantum–infected dogs: asymptomatic I (AD-I: serum negative/PCR+), asymptomatic II (AD-II: serum positive), oligosymptomatic (OD), and symptomatic (SD). Results were compared with those from noninfected dogs (NID). The SD group was found to present a decrease in erythropoietic lineage with concomitant reductions in erythrocytes, hemoglobin, and hematocrit parameters, resulting in anemia. The SD group also had increased neutrophils and precursors and decreased band eosinophils and eosinophils, leading to peripheral blood leucopenia. In the AD-II group, lymphocytosis occurred in both the peripheral blood and the bone marrow compartments. The SD group exhibited lymphocytosis in the bone marrow, with lymphopenia in the peripheral blood. In contrast, the AD-I group, showed no significant changes suggestive of CVL, presenting normal counts in bone marrow and peripheral blood. Our results showed for the first time that important changes in hematopoiesis and hematological parameters occur during ongoing CVL in naturally infected dogs, mainly in symptomatic disease. Taken together, our results based on myelogram and hemogram parameters enable better understanding of the pathogenesis of the anemia, lymphocytosis, and lymphopenia, as well as the leucopenia (eosinopenia and monocytopenia), that contribute to CVL prognosis.
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spelling Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.Leishmania infantumCanine visceral leishmaniasisHematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density in dogs naturally infected by L. infantum. To further characterize these alterations, we evaluated the association between the hematological parameters in bone marrow and peripheral blood alterations in groups of L. infantum–infected dogs: asymptomatic I (AD-I: serum negative/PCR+), asymptomatic II (AD-II: serum positive), oligosymptomatic (OD), and symptomatic (SD). Results were compared with those from noninfected dogs (NID). The SD group was found to present a decrease in erythropoietic lineage with concomitant reductions in erythrocytes, hemoglobin, and hematocrit parameters, resulting in anemia. The SD group also had increased neutrophils and precursors and decreased band eosinophils and eosinophils, leading to peripheral blood leucopenia. In the AD-II group, lymphocytosis occurred in both the peripheral blood and the bone marrow compartments. The SD group exhibited lymphocytosis in the bone marrow, with lymphopenia in the peripheral blood. In contrast, the AD-I group, showed no significant changes suggestive of CVL, presenting normal counts in bone marrow and peripheral blood. Our results showed for the first time that important changes in hematopoiesis and hematological parameters occur during ongoing CVL in naturally infected dogs, mainly in symptomatic disease. Taken together, our results based on myelogram and hemogram parameters enable better understanding of the pathogenesis of the anemia, lymphocytosis, and lymphopenia, as well as the leucopenia (eosinopenia and monocytopenia), that contribute to CVL prognosis.2015-01-21T18:20:27Z2015-01-21T18:20:27Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNICOLATO, R. de C. et al. Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes. Plos One, v. 8, p. e82947, 2013. Disponível em: <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082947>. Acesso em: 12 ago. 2014.1932-6203http://www.repositorio.ufop.br/handle/123456789/4313https://doi.org/10.1371/journal.pone.0082947Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.info:eu-repo/semantics/openAccessNicolato, Roney Luiz de CarvalhoAbreu, Raquel Trópia deRoatt, Bruno MendesSoares, Rodrigo Dian de Oliveira AguiarReis, Levi Eduardo SoaresCarvalho, Maria das GraçasCarneiro, Cláudia MartinsGiunchetti, Rodolfo CordeiroBouillet, Leoneide Érica MaduroLemos, Denise da SilveiraVital, Wendel CouraReis, Alexandre Barbosaengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-11T16:57:59Zoai:repositorio.ufop.br:123456789/4313Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-11T16:57:59Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
title Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
spellingShingle Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
Nicolato, Roney Luiz de Carvalho
Leishmania infantum
Canine visceral leishmaniasis
title_short Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
title_full Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
title_fullStr Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
title_full_unstemmed Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
title_sort Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes.
author Nicolato, Roney Luiz de Carvalho
author_facet Nicolato, Roney Luiz de Carvalho
Abreu, Raquel Trópia de
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Reis, Levi Eduardo Soares
Carvalho, Maria das Graças
Carneiro, Cláudia Martins
Giunchetti, Rodolfo Cordeiro
Bouillet, Leoneide Érica Maduro
Lemos, Denise da Silveira
Vital, Wendel Coura
Reis, Alexandre Barbosa
author_role author
author2 Abreu, Raquel Trópia de
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Reis, Levi Eduardo Soares
Carvalho, Maria das Graças
Carneiro, Cláudia Martins
Giunchetti, Rodolfo Cordeiro
Bouillet, Leoneide Érica Maduro
Lemos, Denise da Silveira
Vital, Wendel Coura
Reis, Alexandre Barbosa
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nicolato, Roney Luiz de Carvalho
Abreu, Raquel Trópia de
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Reis, Levi Eduardo Soares
Carvalho, Maria das Graças
Carneiro, Cláudia Martins
Giunchetti, Rodolfo Cordeiro
Bouillet, Leoneide Érica Maduro
Lemos, Denise da Silveira
Vital, Wendel Coura
Reis, Alexandre Barbosa
dc.subject.por.fl_str_mv Leishmania infantum
Canine visceral leishmaniasis
topic Leishmania infantum
Canine visceral leishmaniasis
description Hematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density in dogs naturally infected by L. infantum. To further characterize these alterations, we evaluated the association between the hematological parameters in bone marrow and peripheral blood alterations in groups of L. infantum–infected dogs: asymptomatic I (AD-I: serum negative/PCR+), asymptomatic II (AD-II: serum positive), oligosymptomatic (OD), and symptomatic (SD). Results were compared with those from noninfected dogs (NID). The SD group was found to present a decrease in erythropoietic lineage with concomitant reductions in erythrocytes, hemoglobin, and hematocrit parameters, resulting in anemia. The SD group also had increased neutrophils and precursors and decreased band eosinophils and eosinophils, leading to peripheral blood leucopenia. In the AD-II group, lymphocytosis occurred in both the peripheral blood and the bone marrow compartments. The SD group exhibited lymphocytosis in the bone marrow, with lymphopenia in the peripheral blood. In contrast, the AD-I group, showed no significant changes suggestive of CVL, presenting normal counts in bone marrow and peripheral blood. Our results showed for the first time that important changes in hematopoiesis and hematological parameters occur during ongoing CVL in naturally infected dogs, mainly in symptomatic disease. Taken together, our results based on myelogram and hemogram parameters enable better understanding of the pathogenesis of the anemia, lymphocytosis, and lymphopenia, as well as the leucopenia (eosinopenia and monocytopenia), that contribute to CVL prognosis.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015-01-21T18:20:27Z
2015-01-21T18:20:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NICOLATO, R. de C. et al. Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes. Plos One, v. 8, p. e82947, 2013. Disponível em: <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082947>. Acesso em: 12 ago. 2014.
1932-6203
http://www.repositorio.ufop.br/handle/123456789/4313
https://doi.org/10.1371/journal.pone.0082947
identifier_str_mv NICOLATO, R. de C. et al. Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantum – infected dogs and related myelogram and hemogram changes. Plos One, v. 8, p. e82947, 2013. Disponível em: <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082947>. Acesso em: 12 ago. 2014.
1932-6203
url http://www.repositorio.ufop.br/handle/123456789/4313
https://doi.org/10.1371/journal.pone.0082947
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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