Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity

Detalhes bibliográficos
Autor(a) principal: Batista, Cassiano Martin
Data de Publicação: 2018
Outros Autores: Kessler, Rafael Luis, Eger, Iriane, Soares, Maurilio José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31687
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
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spelling Batista, Cassiano MartinKessler, Rafael LuisEger, IrianeSoares, Maurilio José2019-02-15T16:53:05Z2019-02-15T16:53:05Z2018BATISTA, Cassiano Martin et al. Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity. BMC Cell Biology, v. 19, n. 19, p. 1-16, 2018.1471-2121https://www.arca.fiocruz.br/handle/icict/3168710.1186/s12860-018-0170-3porBMCTrypanosoma cruziCell DifferentiationEndocytosisLipoylation2-BromopalmitateChagas DiseaseDiferenciación CelularEndocitosisLipoilaciónDiferenciação CelularEndocitoseLipoilaçãoDoença de ChagasTreatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Laboratório de Biotecnologia Celular de Mamíferos. Curitiba, PR, Brasil.Universidade Estadual de Ponta Grossa. Departamento de Biologia Geral. Ponta Grossa, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.The palmitate analogue 2-bromopalmitate (2-BP) is a non-selective membrane tethered cysteine alkylator of many membrane-associated enzymes that in the last years emerged as a general inhibitor of protein S-palmitoylation. Palmitoylation is a post-translational protein modification that adds palmitic acid to a cysteine residue through a thioester linkage, promoting membrane localization, protein stability, regulation of enzymatic activity, and the epigenetic regulation of gene expression. Little is known on such important process in the pathogenic protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. The effect of 2-BP was analyzed on different developmental forms of Trypanosoma cruzi. The IC50/48 h value for culture epimastigotes was estimated as 130 μM. The IC50/24 h value for metacyclic trypomastigotes was 216 nM, while for intracellular amastigotes it was 242 μM and for cell derived trypomasigotes was 262 μM (IC50/24 h). Our data showed that 2-BP altered T. cruzi: 1) morphology, as assessed by bright field, scanning and transmission electron microscopy; 2) mitochondrial membrane potential, as shown by flow cytometry after incubation with rhodamine-123; 3) endocytosis, as seen after incubation with transferrin or albumin and analysis by flow cytometry/fluorescence microscopy; 4) in vitro metacyclogenesis; and 5) infectivity, as shown by host cell infection assays. On the other hand, lipid stress by incubation with palmitate did not alter epimastigote growth, metacyclic trypomastigotes viability or trypomastigote infectivity. Our results indicate that 2-BP inhibits key cellular processes of T. cruzi that may be regulated by palmitoylation of vital proteins and suggest a metacyclic trypomastigote unique target dependency during the parasite development.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/31687/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs12860-018.pdfs12860-018.pdfapplication/pdf4152821https://www.arca.fiocruz.br/bitstream/icict/31687/2/s12860-018.pdf0db94428b5d99a71640072094cccd40fMD52TEXTs12860-018.pdf.txts12860-018.pdf.txtExtracted texttext/plain67378https://www.arca.fiocruz.br/bitstream/icict/31687/3/s12860-018.pdf.txt29d1e81937890b058c85e4bc7e38862dMD53icict/316872019-02-17 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dc.title.pt_BR.fl_str_mv Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
title Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
spellingShingle Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
Batista, Cassiano Martin
Trypanosoma cruzi
Cell Differentiation
Endocytosis
Lipoylation
2-Bromopalmitate
Chagas Disease
Diferenciación Celular
Endocitosis
Lipoilación
Diferenciação Celular
Endocitose
Lipoilação
Doença de Chagas
title_short Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
title_full Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
title_fullStr Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
title_full_unstemmed Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
title_sort Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
author Batista, Cassiano Martin
author_facet Batista, Cassiano Martin
Kessler, Rafael Luis
Eger, Iriane
Soares, Maurilio José
author_role author
author2 Kessler, Rafael Luis
Eger, Iriane
Soares, Maurilio José
author2_role author
author
author
dc.contributor.author.fl_str_mv Batista, Cassiano Martin
Kessler, Rafael Luis
Eger, Iriane
Soares, Maurilio José
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Cell Differentiation
Endocytosis
Lipoylation
2-Bromopalmitate
Chagas Disease
Diferenciación Celular
Endocitosis
Lipoilación
Diferenciação Celular
Endocitose
Lipoilação
Doença de Chagas
dc.subject.en.pt_BR.fl_str_mv Cell Differentiation
Endocytosis
Lipoylation
2-Bromopalmitate
Chagas Disease
dc.subject.es.pt_BR.fl_str_mv Diferenciación Celular
Endocitosis
Lipoilación
dc.subject.decs.pt_BR.fl_str_mv Diferenciação Celular
Endocitose
Lipoilação
Doença de Chagas
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-15T16:53:05Z
dc.date.available.fl_str_mv 2019-02-15T16:53:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BATISTA, Cassiano Martin et al. Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity. BMC Cell Biology, v. 19, n. 19, p. 1-16, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31687
dc.identifier.issn.pt_BR.fl_str_mv 1471-2121
dc.identifier.doi.none.fl_str_mv 10.1186/s12860-018-0170-3
identifier_str_mv BATISTA, Cassiano Martin et al. Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity. BMC Cell Biology, v. 19, n. 19, p. 1-16, 2018.
1471-2121
10.1186/s12860-018-0170-3
url https://www.arca.fiocruz.br/handle/icict/31687
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31687/1/license.txt
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