Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan

Detalhes bibliográficos
Autor(a) principal: David, Mariana Rocha
Data de Publicação: 2016
Outros Autores: Santos, Lilha Maria Barbosa dos, Vicente, Ana Carolina Paulo, Freitas, Rafael Maciel de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17905
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
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spelling David, Mariana RochaSantos, Lilha Maria Barbosa dosVicente, Ana Carolina PauloFreitas, Rafael Maciel de2017-02-28T14:56:31Z2017-02-28T14:56:31Z2016DAVID, Mariana Rocha; et al. Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan. Mem Inst Oswaldo Cruz, v.111, n.9, p.577-587, Sept. 2016.1678-8080https://www.arca.fiocruz.br/handle/icict/1790510.1590/0074-02760160238engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.DengueAedesMicrobiotaDiversidadeAedesMicrobiotadiversitymark-release-recaptureEffects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Mosquito midgut microbiota is a key component of vector competence, as gut bacteria can disturb pathogen development. In this study, we addressed the microbiota composition of Aedes aegypti during its lifespan, under field conditions. We also investigated the possible effects of environment, dietary regime and ageing on the gut community composition. We employed culture independent and dependent approaches to characterise vector microbiota. There was evidence of a lifelong stable core microbiota after mosquitoes were released into an urban settlement, where they presumably fed on a range of vertebrate hosts and carbohydrate sources. This core was formed mainly of bacteria belonging to the genera Pseudomonas, Acinetobacter, Aeromonas and Stenotrophomonas and to the families Oxalobacteraceae, Enterobacteriaceae and Comamonadaceae. We showed that both dietary regime and age were associated with the abundance of some bacterial groups in the Ae. aegypti microbiota. The majority of the bacterial groups we identified have been detected in the midgut of Ae. aegypti from laboratory and wild populations, indicating a possible core microbiota associated with this mosquito species. Our findings suggest that Ae. aegypti harbours a stable bacterial community during its adult life, similar to mosquito populations from distinct geographic areas, which may be further explored for arbovirus biocontrol strategies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17905/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariana_david_etal_IOC_2016.pdfmariana_david_etal_IOC_2016.pdfapplication/pdf1856405https://www.arca.fiocruz.br/bitstream/icict/17905/2/mariana_david_etal_IOC_2016.pdf224608e5ae2825ddb120da52e97ed98cMD52TEXTmariana_david_etal_IOC_2016.pdf.txtmariana_david_etal_IOC_2016.pdf.txtExtracted texttext/plain50133https://www.arca.fiocruz.br/bitstream/icict/17905/3/mariana_david_etal_IOC_2016.pdf.txt5046ba61871f66adabc1fa28470ba791MD53icict/179052018-08-15 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dc.title.pt_BR.fl_str_mv Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
title Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
spellingShingle Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
David, Mariana Rocha
Dengue
Aedes
Microbiota
Diversidade
Aedes
Microbiota
diversity
mark-release-recapture
title_short Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
title_full Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
title_fullStr Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
title_full_unstemmed Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
title_sort Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan
author David, Mariana Rocha
author_facet David, Mariana Rocha
Santos, Lilha Maria Barbosa dos
Vicente, Ana Carolina Paulo
Freitas, Rafael Maciel de
author_role author
author2 Santos, Lilha Maria Barbosa dos
Vicente, Ana Carolina Paulo
Freitas, Rafael Maciel de
author2_role author
author
author
dc.contributor.author.fl_str_mv David, Mariana Rocha
Santos, Lilha Maria Barbosa dos
Vicente, Ana Carolina Paulo
Freitas, Rafael Maciel de
dc.subject.other.pt_BR.fl_str_mv Dengue
Aedes
Microbiota
Diversidade
topic Dengue
Aedes
Microbiota
Diversidade
Aedes
Microbiota
diversity
mark-release-recapture
dc.subject.en.pt_BR.fl_str_mv Aedes
Microbiota
diversity
mark-release-recapture
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-28T14:56:31Z
dc.date.available.fl_str_mv 2017-02-28T14:56:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DAVID, Mariana Rocha; et al. Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan. Mem Inst Oswaldo Cruz, v.111, n.9, p.577-587, Sept. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17905
dc.identifier.issn.pt_BR.fl_str_mv 1678-8080
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160238
identifier_str_mv DAVID, Mariana Rocha; et al. Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan. Mem Inst Oswaldo Cruz, v.111, n.9, p.577-587, Sept. 2016.
1678-8080
10.1590/0074-02760160238
url https://www.arca.fiocruz.br/handle/icict/17905
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17905/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/17905/2/mariana_david_etal_IOC_2016.pdf
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