Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17956 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. |
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Barletta, Ana Beatriz FerreiraSilva, Maria Clara L. NascimentoTalyuli, Octávio A. C.Oliveira, José Henrique M.Pereira, Luiza Oliveira RamosOliveira, Pedro L.Sorgine, Marcos Henrique F.2017-03-07T12:21:23Z2017-03-07T12:21:23Z2017BARLETTA, Ana Beatriz Ferreira; et al. Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti. Parasites & Vectors, v.10:103, 9p, 2017.1756-3305https://www.arca.fiocruz.br/handle/icict/1795610.1186/s13071-017-2040-9engBioMed CentralAedes aegyptiVírus SindbisResposta imuneMicrobiotaZika virusDengueChikungunyaAedes aegyptiSindbis virusImmune responseIMDMicrobiotaZika virusDengueChikungunyaMicrobiota activates IMD pathway and limits Sindbis infection in Aedes aegyptiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmaniose. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Background: Aedes aegypti is the main vector of important arboviruses such as dengue, Zika and chikungunya. During infections mosquitoes can activate the immune pathways Toll, IMD and JAK/STAT to limit pathogen replication. Results: Here, we evaluate the immune response profile of Ae. aegypti against Sindbis virus (SINV). We analyzed gene expression of components of Toll, IMD and JAK/STAT pathways and showed that a blood meal and virus infection upregulated aaREL2 in a microbiota-dependent fashion, since this induction was prevented by antibiotic. The presence of the microbiota activates IMD and impaired the replication of SINV in the midgut. Constitutive activation of the IMD pathway, by Caspar depletion, leads to a decrease in microbiota levels and an increase in SINV loads. Conclusion: Together, these results suggest that a blood meal is able to activate innate immune pathways, through a nutrient induced growth of microbiota, leading to upregulation of aaREL2 and IMD activation. Microbiota levels seemed to have a reciprocal interaction, where the proliferation of the microbiota activates IMD pathway that in turn controls bacterial levels, allowing SINV replication in Ae. aegypti mosquitoes. The activation of the IMD pathway seems to have an indirect effect in SINV levels that is induced by the microbiota.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17956/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALluiza_pereira_etal_IOC_2017.pdfluiza_pereira_etal_IOC_2017.pdfapplication/pdf946502https://www.arca.fiocruz.br/bitstream/icict/17956/2/luiza_pereira_etal_IOC_2017.pdf9fbc3fe71c26955f1174456ceca1c987MD5213071_2017_2040_MOESM1_ESM.docx13071_2017_2040_MOESM1_ESM.docxMaterial Suplementarapplication/vnd.openxmlformats-officedocument.wordprocessingml.document13720https://www.arca.fiocruz.br/bitstream/icict/17956/3/13071_2017_2040_MOESM1_ESM.docxd2193510a889212ed1d4b97de14712e3MD53TEXTluiza_pereira_etal_IOC_2017.pdf.txtluiza_pereira_etal_IOC_2017.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| title |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| spellingShingle |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti Barletta, Ana Beatriz Ferreira Aedes aegypti Vírus Sindbis Resposta imune Microbiota Zika virus Dengue Chikungunya Aedes aegypti Sindbis virus Immune response IMD Microbiota Zika virus Dengue Chikungunya |
| title_short |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| title_full |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| title_fullStr |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| title_full_unstemmed |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| title_sort |
Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
| author |
Barletta, Ana Beatriz Ferreira |
| author_facet |
Barletta, Ana Beatriz Ferreira Silva, Maria Clara L. Nascimento Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. |
| author_role |
author |
| author2 |
Silva, Maria Clara L. Nascimento Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Barletta, Ana Beatriz Ferreira Silva, Maria Clara L. Nascimento Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes aegypti Vírus Sindbis Resposta imune Microbiota Zika virus Dengue Chikungunya |
| topic |
Aedes aegypti Vírus Sindbis Resposta imune Microbiota Zika virus Dengue Chikungunya Aedes aegypti Sindbis virus Immune response IMD Microbiota Zika virus Dengue Chikungunya |
| dc.subject.en.pt_BR.fl_str_mv |
Aedes aegypti Sindbis virus Immune response IMD Microbiota Zika virus Dengue Chikungunya |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil. |
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2017 |
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2017-03-07T12:21:23Z |
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2017-03-07T12:21:23Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
BARLETTA, Ana Beatriz Ferreira; et al. Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti. Parasites & Vectors, v.10:103, 9p, 2017. |
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https://www.arca.fiocruz.br/handle/icict/17956 |
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1756-3305 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-017-2040-9 |
| identifier_str_mv |
BARLETTA, Ana Beatriz Ferreira; et al. Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti. Parasites & Vectors, v.10:103, 9p, 2017. 1756-3305 10.1186/s13071-017-2040-9 |
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BioMed Central |
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BioMed Central |
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