The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37922 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Mörking, Patricia AlvesRampazzo, Rita de Cássia PontelloWalrad, PegineProbst, Christian MacagnanSoares, Maurilio JoséGradia, Daniela FioriPavoni, Daniela ParadaKrieger, Marco AurélioMatthews, KeithGoldenberg, SamuelFragoso, Stenio PerdigãoDallagiovanna, Bruno2019-12-12T19:46:38Z2019-12-12T19:46:38Z2012MÖRKING, Patrícia Alves et al. The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 107, n. 6, p. 790-799, 2012.1678-8060https://www.arca.fiocruz.br/handle/icict/3792210.1590/s0074-02762012000600014engFundação Oswaldo Cruz. Instituto Oswaldo CruzTrypanosoma cruziZinc FingersRNA-Binding ProteinsDedos de ZincProteínas de Unión al ARNDedos de ZincoProteínas de Ligação a RNAThe zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Centre for Immunity, Infection and Evolution. School of Biological Sciences. University of Edinburgh. Edinburgh, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Centre for Immunity, Infection and Evolution. School of Biological Sciences. University of Edinburgh. Edinburgh, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Trypanosomes are parasitic protozoa in which gene expression is primarily controlled through the regulation of mRNA stability and translation. This post-transcriptional control is mediated by various families of RNA-binding proteins, including those with zinc finger CCCH motifs. CCCH zinc finger proteins have been shown to be essential to differentiation events in trypanosomatid parasites. Here, we functionally characterise TcZFP2 as a predicted post-transcriptional regulator of differentiation in Trypanosoma cruzi. This protein was detected in cell culture-derived amastigotes and trypomastigotes, but it was present in smaller amounts in metacyclic trypomastigote forms of T. cruzi. We use an optimised recombinant RNA immunopreciptation followed by microarray analysis assay to identify TcZFP2 target mRNAs. We further demonstrate that TcZFP2 binds an A-rich sequence in which the adenosine residue repeats are essential for high-affinity recognition. An analysis of the expression profiles of the genes encoding the TcZFP2-associated mRNAs throughout the parasite life cycle by microarray hybridisation showed that most of the associated mRNAs were upregulated in the metacyclic trypomastigote forms, also suggesting a role for TcZFP2 in metacyclic trypomastigote differentiation. Knockdown of the orthologous Trypanosoma brucei protein levels showed ZFP2 to be a positive regulator of specific target mRNA abundance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/37922/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL14ok.pdf14ok.pdfapplication/pdf3289252https://www.arca.fiocruz.br/bitstream/icict/37922/2/14ok.pdf83d220622cd86254eb07b67132ef9eaaMD52TEXT14ok.pdf.txt14ok.pdf.txtExtracted texttext/plain61350https://www.arca.fiocruz.br/bitstream/icict/37922/3/14ok.pdf.txta2f2fe6f136405510fc254ef656c9724MD53icict/379222019-12-14 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| dc.title.pt_BR.fl_str_mv |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| title |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| spellingShingle |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis Mörking, Patricia Alves Trypanosoma cruzi Zinc Fingers RNA-Binding Proteins Dedos de Zinc Proteínas de Unión al ARN Dedos de Zinco Proteínas de Ligação a RNA |
| title_short |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| title_full |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| title_fullStr |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| title_full_unstemmed |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| title_sort |
The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis |
| author |
Mörking, Patricia Alves |
| author_facet |
Mörking, Patricia Alves Rampazzo, Rita de Cássia Pontello Walrad, Pegine Probst, Christian Macagnan Soares, Maurilio José Gradia, Daniela Fiori Pavoni, Daniela Parada Krieger, Marco Aurélio Matthews, Keith Goldenberg, Samuel Fragoso, Stenio Perdigão Dallagiovanna, Bruno |
| author_role |
author |
| author2 |
Rampazzo, Rita de Cássia Pontello Walrad, Pegine Probst, Christian Macagnan Soares, Maurilio José Gradia, Daniela Fiori Pavoni, Daniela Parada Krieger, Marco Aurélio Matthews, Keith Goldenberg, Samuel Fragoso, Stenio Perdigão Dallagiovanna, Bruno |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mörking, Patricia Alves Rampazzo, Rita de Cássia Pontello Walrad, Pegine Probst, Christian Macagnan Soares, Maurilio José Gradia, Daniela Fiori Pavoni, Daniela Parada Krieger, Marco Aurélio Matthews, Keith Goldenberg, Samuel Fragoso, Stenio Perdigão Dallagiovanna, Bruno |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi |
| topic |
Trypanosoma cruzi Zinc Fingers RNA-Binding Proteins Dedos de Zinc Proteínas de Unión al ARN Dedos de Zinco Proteínas de Ligação a RNA |
| dc.subject.en.pt_BR.fl_str_mv |
Zinc Fingers RNA-Binding Proteins |
| dc.subject.es.pt_BR.fl_str_mv |
Dedos de Zinc Proteínas de Unión al ARN |
| dc.subject.decs.pt_BR.fl_str_mv |
Dedos de Zinco Proteínas de Ligação a RNA |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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2012 |
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2012 |
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2019-12-12T19:46:38Z |
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2019-12-12T19:46:38Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MÖRKING, Patrícia Alves et al. The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 107, n. 6, p. 790-799, 2012. |
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https://www.arca.fiocruz.br/handle/icict/37922 |
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1678-8060 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/s0074-02762012000600014 |
| identifier_str_mv |
MÖRKING, Patrícia Alves et al. The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis. Memórias do Instituto Oswaldo Cruz. Rio de Janeiro, v. 107, n. 6, p. 790-799, 2012. 1678-8060 10.1590/s0074-02762012000600014 |
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