Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.

Detalhes bibliográficos
Autor(a) principal: Araujo, Adriano Fernando da Silva
Data de Publicação: 2014
Outros Autores: Oliveira, Gabriel de, Vasconcelos, Juliana Fraga, Ersching, Jonatan, Dominguez, Mariana Ribeiro, Vasconcelos, José Ronnie Carvalho de, Machado, Alexandre Vieira, Gazzinelli, Ricardo Tostes, Romero, Oscar Bruna, Soares, Milena Botelho Pereira, Rodrigues, Maurício Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8155
Resumo: Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
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spelling Araujo, Adriano Fernando da SilvaOliveira, Gabriel deVasconcelos, Juliana FragaErsching, JonatanDominguez, Mariana RibeiroVasconcelos, José Ronnie Carvalho deMachado, Alexandre VieiraGazzinelli, Ricardo TostesRomero, Oscar BrunaSoares, Milena Botelho PereiraRodrigues, Maurício Martins2014-08-08T17:51:54Z2014-08-08T17:51:54Z2014ARAÚJO, Adriano Fernando da Silva et al. Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi. Mediators of Inflammation, 2014, p. 605023, 2014.1466-1861https://www.arca.fiocruz.br/handle/icict/815510.1155/2014/605023engHindawi Publishing CorporationTrypanosoma cruziDoença de ChagasVacinaçãoAnimaisCamundongosPatologiaGenetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEscola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Biologia Celular. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilEscola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilEscola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilEscola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil / Departamento de Biociências. Instituto de Saúde e Sociedade - UNIFESP. Santos, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, BH, Brasil / University of Massachusetts Medical School. Division of Infectious Disease and Immunology. Department of Medicine. Worcester, MA, USAUniversidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianopolis, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilEscola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilIn earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replicationdefective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged withmyotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing themurine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptiblemale mice against acute lethal infections with these parasites. The prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAraujo A F Genetic vaccination....pdfAraujo A F Genetic vaccination....pdfapplication/pdf1985863https://www.arca.fiocruz.br/bitstream/icict/8155/1/Araujo%20A%20F%20Genetic%20vaccination....pdf152a600e90eae679adc9f1fbbda2d1f7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8155/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAraujo A F Genetic vaccination....pdf.txtAraujo A F Genetic vaccination....pdf.txtExtracted texttext/plain49860https://www.arca.fiocruz.br/bitstream/icict/8155/3/Araujo%20A%20F%20Genetic%20vaccination....pdf.txtdd8c7c3345a8b7e1cf8ac0d369d98a8fMD53icict/81552023-09-14 16:02:21.359oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T19:02:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
title Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
spellingShingle Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
Araujo, Adriano Fernando da Silva
Trypanosoma cruzi
Doença de Chagas
Vacinação
Animais
Camundongos
Patologia
title_short Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
title_full Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
title_fullStr Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
title_full_unstemmed Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
title_sort Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi.
author Araujo, Adriano Fernando da Silva
author_facet Araujo, Adriano Fernando da Silva
Oliveira, Gabriel de
Vasconcelos, Juliana Fraga
Ersching, Jonatan
Dominguez, Mariana Ribeiro
Vasconcelos, José Ronnie Carvalho de
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Soares, Milena Botelho Pereira
Rodrigues, Maurício Martins
author_role author
author2 Oliveira, Gabriel de
Vasconcelos, Juliana Fraga
Ersching, Jonatan
Dominguez, Mariana Ribeiro
Vasconcelos, José Ronnie Carvalho de
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Soares, Milena Botelho Pereira
Rodrigues, Maurício Martins
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Adriano Fernando da Silva
Oliveira, Gabriel de
Vasconcelos, Juliana Fraga
Ersching, Jonatan
Dominguez, Mariana Ribeiro
Vasconcelos, José Ronnie Carvalho de
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Soares, Milena Botelho Pereira
Rodrigues, Maurício Martins
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Vacinação
Animais
Camundongos
Patologia
topic Trypanosoma cruzi
Doença de Chagas
Vacinação
Animais
Camundongos
Patologia
description Escola Paulista de Medicina - UNIFESP. Centro de Terapia Celular e Molecular - CTCMol. São Paulo, SP, Brasil / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departmento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-08-08T17:51:54Z
dc.date.available.fl_str_mv 2014-08-08T17:51:54Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAÚJO, Adriano Fernando da Silva et al. Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi. Mediators of Inflammation, 2014, p. 605023, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8155
dc.identifier.issn.none.fl_str_mv 1466-1861
dc.identifier.doi.none.fl_str_mv 10.1155/2014/605023
identifier_str_mv ARAÚJO, Adriano Fernando da Silva et al. Genetic vaccination against experimental infection with myotropic parasite strains of Trypanosoma cruzi. Mediators of Inflammation, 2014, p. 605023, 2014.
1466-1861
10.1155/2014/605023
url https://www.arca.fiocruz.br/handle/icict/8155
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
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