Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils

Detalhes bibliográficos
Autor(a) principal: Martins, Paulo Henrique Rosa
Data de Publicação: 2023
Outros Autores: Rabinovitch, Leon, Orem, Juliana Capela de, Silva, Waldeyr Mendes C., Mesquita, Felipe de Araujo, Magalhães, Maria Ines Andre de, Cavalcante, Danilo de Andrade, Vivoni, Adriana Marcos, Oliveira, Edmar Justo de, Lima, Vera Cristina Pessoa de, Brito, Josiane Teixeira, Souza, Marlene Teixeira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3897/neotropical.18.e86548
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57653
Resumo: Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.
id CRUZ_9b4b2797c22a02b1610dceb75e6b764f
oai_identifier_str oai:www.arca.fiocruz.br:icict/57653
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Martins, Paulo Henrique RosaRabinovitch, LeonOrem, Juliana Capela deSilva, Waldeyr Mendes C.Mesquita, Felipe de AraujoMagalhães, Maria Ines Andre deCavalcante, Danilo de AndradeVivoni, Adriana MarcosOliveira, Edmar Justo deLima, Vera Cristina Pessoa deBrito, Josiane TeixeiraSouza, Marlene Teixeira de2023-04-03T11:49:41Z2023-04-03T11:49:41Z2023MARTINS, Paulo Henrique Rosa et al. Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils. Neotropical Biology and Conservation, v.18, n.1, p. 53-72, Feb. 2023.2236-3777https://www.arca.fiocruz.br/handle/icict/5765310.3897/neotropical.18.e86548engPensoftBacilosIdentificação bacterianaMetabolismo bacterianoFirmicutesFenotipagemTaxonomiaEndosporulaçãoBacillalesBacterial identificationBacterial metabolismFirmicutesPhenotypingTaxonomyEndosporulationBiochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.Instituto Federal de Goias. Formosa, GO, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.The aerobic endospore-forming bacteria (AEFB) comprise species of Bacillus and related genera and have long been regarded as prominent constituents of the soil bacterial community. The wide diversity of AEFB renders appropriate categorisation and generalisations a challenging task. We previously isolated 312 AEFB strains from Brazilian soils that we designated SDF (Solo do Distrito Federal) strains. To better understand the SDF diversity and explore their biotechnological potential, we addressed the biochemical and physiological profiles of these 312 environmental strains by performing 30 tests in this work. Of these, the 16S rRNA gene sequences segregated 238 SDF strains into four genera in the family Bacillaceae and two in the Paenibacillaceae. Bacillus spp. were the most prevalent, followed by species of Paenibacillus. We summarised the phenotypic test relationships among selected SDF strains using a Pearson correlation-based clustering represented in heatmaps. In practice, biochemical and physiological profiles are often less discriminatory than molecular data and may be unstable because of the loss of traits. Although these test reactions are not universally positive or negative within species, they may define biotypes and be efficient strain markers, enhancing the accuracy of unknown sample identification. It can also help select the most representative phenotypes of samples. Along with the other phenotypic and genotypic data, the present results are of great importance for the robust classification of the SDF strains within the scope of the polyphasic approach.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57653/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLeonRabinovich_AdrianaVivoni_etal_IOC_2023.pdfLeonRabinovich_AdrianaVivoni_etal_IOC_2023.pdfapplication/pdf488675https://www.arca.fiocruz.br/bitstream/icict/57653/2/LeonRabinovich_AdrianaVivoni_etal_IOC_2023.pdf126695441284a533c9234d3813d4d28aMD52icict/576532023-09-14 16:02:14.212oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T19:02:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
title Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
spellingShingle Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
Martins, Paulo Henrique Rosa
Bacilos
Identificação bacteriana
Metabolismo bacteriano
Firmicutes
Fenotipagem
Taxonomia
Endosporulação
Bacillales
Bacterial identification
Bacterial metabolism
Firmicutes
Phenotyping
Taxonomy
Endosporulation
title_short Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
title_full Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
title_fullStr Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
title_full_unstemmed Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
title_sort Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils
author Martins, Paulo Henrique Rosa
author_facet Martins, Paulo Henrique Rosa
Rabinovitch, Leon
Orem, Juliana Capela de
Silva, Waldeyr Mendes C.
Mesquita, Felipe de Araujo
Magalhães, Maria Ines Andre de
Cavalcante, Danilo de Andrade
Vivoni, Adriana Marcos
Oliveira, Edmar Justo de
Lima, Vera Cristina Pessoa de
Brito, Josiane Teixeira
Souza, Marlene Teixeira de
author_role author
author2 Rabinovitch, Leon
Orem, Juliana Capela de
Silva, Waldeyr Mendes C.
Mesquita, Felipe de Araujo
Magalhães, Maria Ines Andre de
Cavalcante, Danilo de Andrade
Vivoni, Adriana Marcos
Oliveira, Edmar Justo de
Lima, Vera Cristina Pessoa de
Brito, Josiane Teixeira
Souza, Marlene Teixeira de
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Paulo Henrique Rosa
Rabinovitch, Leon
Orem, Juliana Capela de
Silva, Waldeyr Mendes C.
Mesquita, Felipe de Araujo
Magalhães, Maria Ines Andre de
Cavalcante, Danilo de Andrade
Vivoni, Adriana Marcos
Oliveira, Edmar Justo de
Lima, Vera Cristina Pessoa de
Brito, Josiane Teixeira
Souza, Marlene Teixeira de
dc.subject.other.en_US.fl_str_mv Bacilos
Identificação bacteriana
Metabolismo bacteriano
Firmicutes
Fenotipagem
Taxonomia
Endosporulação
topic Bacilos
Identificação bacteriana
Metabolismo bacteriano
Firmicutes
Fenotipagem
Taxonomia
Endosporulação
Bacillales
Bacterial identification
Bacterial metabolism
Firmicutes
Phenotyping
Taxonomy
Endosporulation
dc.subject.en.en_US.fl_str_mv Bacillales
Bacterial identification
Bacterial metabolism
Firmicutes
Phenotyping
Taxonomy
Endosporulation
description Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-03T11:49:41Z
dc.date.available.fl_str_mv 2023-04-03T11:49:41Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Paulo Henrique Rosa et al. Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils. Neotropical Biology and Conservation, v.18, n.1, p. 53-72, Feb. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57653
dc.identifier.issn.en_US.fl_str_mv 2236-3777
dc.identifier.doi.none.fl_str_mv 10.3897/neotropical.18.e86548
identifier_str_mv MARTINS, Paulo Henrique Rosa et al. Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils. Neotropical Biology and Conservation, v.18, n.1, p. 53-72, Feb. 2023.
2236-3777
10.3897/neotropical.18.e86548
url https://www.arca.fiocruz.br/handle/icict/57653
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Pensoft
publisher.none.fl_str_mv Pensoft
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57653/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57653/2/LeonRabinovich_AdrianaVivoni_etal_IOC_2023.pdf
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
126695441284a533c9234d3813d4d28a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1822791674428915712

Registros relacionados