Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Edward José
Data de Publicação: 2022
Outros Autores: Freire, Mariana Lourenço, Rego, Felipe Dutra, Lopes, Karine Ferreira, Coutinho, Lucélia Antunes, Grenfell, Rafaella Fortini Queiroz, Avelar, Daniel Moreira, Cota, Gláucia Fernandes, Xavier, Marcelo Antônio Pascoal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56022
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
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spelling Oliveira, Edward JoséFreire, Mariana LourençoRego, Felipe DutraLopes, Karine FerreiraCoutinho, Lucélia AntunesGrenfell, Rafaella Fortini QueirozAvelar, Daniel MoreiraCota, Gláucia FernandesXavier, Marcelo Antônio Pascoal2022-12-16T17:10:38Z2022-12-16T17:10:38Z2022FREIRE, Mariana Lourenço et al. Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis. Front Microbiol., v. 12, 790906, 2022. doi: 10.3389/fmicb.2021.790906.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/56022engFrontiers Research FoundationAnti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil.Cutaneous leishmaniasis (CL) remains a globally spreading public health problem. Among Latin America countries, Brazil has the greatest number of recorded CL cases with several Leishmania species being associated with human cases. Laboratory diagnosis is one of the major challenges to disease control due to the low accuracy of parasitological techniques, the restricted use of molecular techniques, and the importance of differential diagnosis with regard to several dermatological and systemic diseases. In response, we have developed and validated an immunohistochemistry (IHC) technique for CL diagnosis using anti-mTXNPx monoclonal antibody (mAb). Recombinant Leishmania-mTXNPx was produced and used as an immunogen for mAb production through the somatic hybridization technique. The viability of mAb labeling of Leishmania amastigotes was tested by IHC performed with skin biopsies from hamsters experimentally infected with Leishmania amazonensis, Leishmania braziliensis, and Leishmania guyanensis. The enzymes horseradish peroxidase (IHC-HRP) and alkaline phosphatase (IHC-AP), both biotin-free polymer detection systems, were used in the standardization step. The IHC was further validated with skin biopsies from 49 CL patients diagnosed by clinical examination and quantitative real-time polymerase chain reaction and from 37 patients presenting other dermatological infectious diseases. Other parasitological techniques, such as direct examination and culture, were also performed for confirmed CL patients. Histopathology and IHC were performed for all included patients. Overall, the highest sensitivity was observed for IHC-AP (85.7%), followed by IHC-HRP (79.6%), direct examination (77.6%), histopathological examination (HE; 65.3%), and in vitro culture (49%). Only IHC and HE presented specificity over 90% and were able to detect CL patients regardless of parasite burden (odds ratio > 1.94; 95%CI: 0.34-11.23). A significant increase in positivity rates was observed when IHC-AP was combined with direct examination (95.9%) and HE (93.9%). The IHC techniques evaluated in here detected the main Leishmania species causing CL in Brazil and can support diagnostic strategies for controlling this neglected disease, especially if used in combination with other approaches for an integrative laboratorial diagnosis.cutaneous leishmaniasis;diagnosisimmunohistochemistrymTXNPxmonoclonal antibodyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56022/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnti-mitochondrial Tryparedoxin Peroxidase.pdfAnti-mitochondrial Tryparedoxin Peroxidase.pdfapplication/pdf12071134https://www.arca.fiocruz.br/bitstream/icict/56022/2/Anti-mitochondrial%20Tryparedoxin%20Peroxidase.pdfd60109a30ea3a5ff2f9f487db995d7cfMD52icict/560222022-12-16 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dc.title.en_US.fl_str_mv Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
title Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
spellingShingle Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
Oliveira, Edward José
cutaneous leishmaniasis;
diagnosis
immunohistochemistry
mTXNPx
monoclonal antibody
title_short Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
title_full Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
title_fullStr Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
title_full_unstemmed Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
title_sort Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis
author Oliveira, Edward José
author_facet Oliveira, Edward José
Freire, Mariana Lourenço
Rego, Felipe Dutra
Lopes, Karine Ferreira
Coutinho, Lucélia Antunes
Grenfell, Rafaella Fortini Queiroz
Avelar, Daniel Moreira
Cota, Gláucia Fernandes
Xavier, Marcelo Antônio Pascoal
author_role author
author2 Freire, Mariana Lourenço
Rego, Felipe Dutra
Lopes, Karine Ferreira
Coutinho, Lucélia Antunes
Grenfell, Rafaella Fortini Queiroz
Avelar, Daniel Moreira
Cota, Gláucia Fernandes
Xavier, Marcelo Antônio Pascoal
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Edward José
Freire, Mariana Lourenço
Rego, Felipe Dutra
Lopes, Karine Ferreira
Coutinho, Lucélia Antunes
Grenfell, Rafaella Fortini Queiroz
Avelar, Daniel Moreira
Cota, Gláucia Fernandes
Xavier, Marcelo Antônio Pascoal
dc.subject.en.en_US.fl_str_mv cutaneous leishmaniasis;
diagnosis
immunohistochemistry
mTXNPx
monoclonal antibody
topic cutaneous leishmaniasis;
diagnosis
immunohistochemistry
mTXNPx
monoclonal antibody
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-12-16T17:10:38Z
dc.date.available.fl_str_mv 2022-12-16T17:10:38Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREIRE, Mariana Lourenço et al. Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis. Front Microbiol., v. 12, 790906, 2022. doi: 10.3389/fmicb.2021.790906.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56022
dc.identifier.issn.en_US.fl_str_mv 1664-302X
identifier_str_mv FREIRE, Mariana Lourenço et al. Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis. Front Microbiol., v. 12, 790906, 2022. doi: 10.3389/fmicb.2021.790906.
1664-302X
url https://www.arca.fiocruz.br/handle/icict/56022
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56022/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56022/2/Anti-mitochondrial%20Tryparedoxin%20Peroxidase.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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