PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7262 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Santos, Diego MouraCarneiro, Marcia WeberMoura, Tatiana Rodrigues deSoto, ManuelLuz, Nívea FariasPrates, Deboraci BritoIrache, Juan ManuelBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral Netto, ManoelEspuelas, SocorroBorges, Valeria de MatosOliveira, Camila Indiani de2013-11-26T14:04:47Z2013-11-26T14:04:47Z2013SANTOS, D. M. et al. PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 9, p. 985–995, 2013.1549-9634https://www.arca.fiocruz.br/handle/icict/726210.1016/j.nano.2013.04.003engElsevierPLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmaniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autonoma de Madrid. Centro de Biología Molecular Severo Ochoa. Departamento de Biologia Molecular. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad de Navarra. Instituto de Salud Tropical. Pamplona, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrazilUniversidad de Navarra. Instituto de Salud Tropical. Pamplona, Spain / Universidad de Navarra. Facultad de Farmacia. Departamento de Farmacia y Tecnología Farmacéutica. Pamplona, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrazilWe recently demonstrated that immunization with polyester poly(lactide-co-glycolide acid) (PLGA) nanoparticles loaded with the 11-kDa Leishmania vaccine candidate kinetoplastid membrane protein 11 (KMP-11) significantly reduced parasite load in vivo. Presently, we explored the ability of the recombinant PLGA nanoparticles to stimulate innate responses in macrophages and the outcome of infection with Leishmania braziliensis in vitro. Incubation of macrophages with KMP-11-loaded PLGA nanoparticles significantly decreased parasite load. In parallel, we observed the augmented production of nitric oxide, superoxide, TNF-α and IL-6. An increased release of CCL2/MCP-1 and CXCL1/KC was also observed, resulting in macrophage and neutrophil recruitment in vitro. Lastly, the incubation of macrophages with KMP-11-loaded PLGA nanoparticles triggered the activation of caspase-1 and the secretion of IL-1β and IL-18, suggesting inflammasome participation. Inhibition of caspase-1 significantly increased the parasite load. We conclude that KMP-11-loaded PLGA nanoparticles promote the killing of intracellular Leishmania parasites through the induction of potent innate responses.LeishmaniaPLGANanoparticleMacrophageInnate responseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSantos D M PLGA nanoparticles....pdfSantos D M PLGA nanoparticles....pdfapplication/pdf1378587https://www.arca.fiocruz.br/bitstream/icict/7262/1/Santos%20D%20M%20%20PLGA%20nanoparticles....pdf7f7ed57efe858a0fe0a69d986cc9e5a5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7262/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSantos D M PLGA nanoparticles....pdf.txtSantos D M PLGA nanoparticles....pdf.txtExtracted texttext/plain48259https://www.arca.fiocruz.br/bitstream/icict/7262/5/Santos%20D%20M%20%20PLGA%20nanoparticles....pdf.txt5b878332f0085a9cf19c9937b00d3a78MD55THUMBNAILSantos D M PLGA nanoparticles....pdf.jpgSantos D M PLGA nanoparticles....pdf.jpgGenerated Thumbnailimage/jpeg2271https://www.arca.fiocruz.br/bitstream/icict/7262/4/Santos%20D%20M%20%20PLGA%20nanoparticles....pdf.jpg7cd34a22d8f411cdd3a4b5fa217ef390MD54icict/72622023-03-15 14:33:03.425oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| title |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| spellingShingle |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania Santos, Diego Moura Leishmania PLGA Nanoparticle Macrophage Innate response |
| title_short |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| title_full |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| title_fullStr |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| title_full_unstemmed |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| title_sort |
PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
| author |
Santos, Diego Moura |
| author_facet |
Santos, Diego Moura Carneiro, Marcia Weber Moura, Tatiana Rodrigues de Soto, Manuel Luz, Nívea Farias Prates, Deboraci Brito Irache, Juan Manuel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel Espuelas, Socorro Borges, Valeria de Matos Oliveira, Camila Indiani de |
| author_role |
author |
| author2 |
Carneiro, Marcia Weber Moura, Tatiana Rodrigues de Soto, Manuel Luz, Nívea Farias Prates, Deboraci Brito Irache, Juan Manuel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel Espuelas, Socorro Borges, Valeria de Matos Oliveira, Camila Indiani de |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Diego Moura Carneiro, Marcia Weber Moura, Tatiana Rodrigues de Soto, Manuel Luz, Nívea Farias Prates, Deboraci Brito Irache, Juan Manuel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel Espuelas, Socorro Borges, Valeria de Matos Oliveira, Camila Indiani de |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania PLGA Nanoparticle Macrophage Innate response |
| topic |
Leishmania PLGA Nanoparticle Macrophage Innate response |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
| publishDate |
2013 |
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2013-11-26T14:04:47Z |
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2013-11-26T14:04:47Z |
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2013 |
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info:eu-repo/semantics/article |
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SANTOS, D. M. et al. PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 9, p. 985–995, 2013. |
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1549-9634 |
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10.1016/j.nano.2013.04.003 |
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SANTOS, D. M. et al. PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 9, p. 985–995, 2013. 1549-9634 10.1016/j.nano.2013.04.003 |
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