PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania

Detalhes bibliográficos
Autor(a) principal: Santos, Diego M.
Data de Publicação: 2013
Outros Autores: Carneiro, Marcia W., Moura, Tatiana Rodrigues de, Soto, Manuel, Luz, Nívea Farias, Prates, Deboraci Brito, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15887
Resumo: Texto completo: acesso restrito. p. 985–995
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spelling Santos, Diego M.Carneiro, Marcia W.Moura, Tatiana Rodrigues deSoto, ManuelLuz, Nívea FariasPrates, Deboraci BritoBarral, Aldina Maria PradoSantos, Diego M.Carneiro, Marcia W.Moura, Tatiana Rodrigues deSoto, ManuelLuz, Nívea FariasPrates, Deboraci BritoBarral, Aldina Maria Prado2014-09-09T14:49:36Z20131549-9634http://repositorio.ufba.br/ri/handle/ri/15887v. 9, n. 7Texto completo: acesso restrito. p. 985–995We recently demonstrated that immunization with polyester poly(lactide-co-glycolide acid) (PLGA) nanoparticles loaded with the 11-kDa Leishmania vaccine candidate kinetoplastid membrane protein 11 (KMP-11) significantly reduced parasite load in vivo. Presently, we explored the ability of the recombinant PLGA nanoparticles to stimulate innate responses in macrophages and the outcome of infection with Leishmania braziliensis in vitro. Incubation of macrophages with KMP-11-loaded PLGA nanoparticles significantly decreased parasite load. In parallel, we observed the augmented production of nitric oxide, superoxide, TNF-α and IL-6. An increased release of CCL2/MCP-1 and CXCL1/KC was also observed, resulting in macrophage and neutrophil recruitment in vitro. Lastly, the incubation of macrophages with KMP-11-loaded PLGA nanoparticles triggered the activation of caspase-1 and the secretion of IL-1β and IL-18, suggesting inflammasome participation. Inhibition of caspase-1 significantly increased the parasite load. We conclude that KMP-11-loaded PLGA nanoparticles promote the killing of intracellular Leishmania parasites through the induction of potent innate responses.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-29T14:11:17Z No. of bitstreams: 1 1-s2.0-S1549963413001755-main.pdf: 1378587 bytes, checksum: 7f7ed57efe858a0fe0a69d986cc9e5a5 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-09-09T14:49:36Z (GMT) No. of bitstreams: 1 1-s2.0-S1549963413001755-main.pdf: 1378587 bytes, checksum: 7f7ed57efe858a0fe0a69d986cc9e5a5 (MD5)Made available in DSpace on 2014-09-09T14:49:36Z (GMT). No. of bitstreams: 1 1-s2.0-S1549963413001755-main.pdf: 1378587 bytes, checksum: 7f7ed57efe858a0fe0a69d986cc9e5a5 (MD5) Previous issue date: 2013http://dx.doi.org/10.1016/j.nano.2013.04.003reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmaniaPLGANanoparticleMacrophageInnate responsePLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of LeishmaniaNanomedicine: Nanotechnology, Biology and Medicineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S1549963413001755-main.pdf1-s2.0-S1549963413001755-main.pdfapplication/pdf1378587https://repositorio.ufba.br/bitstream/ri/15887/1/1-s2.0-S1549963413001755-main.pdf7f7ed57efe858a0fe0a69d986cc9e5a5MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15887/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1-s2.0-S1549963413001755-main.pdf.txt1-s2.0-S1549963413001755-main.pdf.txtExtracted texttext/plain48228https://repositorio.ufba.br/bitstream/ri/15887/3/1-s2.0-S1549963413001755-main.pdf.txt7b90db201ca0571d7b7cb2b65ae0fefdMD53ri/158872022-07-05 14:03:32.24oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:32Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
dc.title.alternative.pt_BR.fl_str_mv Nanomedicine: Nanotechnology, Biology and Medicine
title PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
spellingShingle PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
Santos, Diego M.
Leishmania
PLGA
Nanoparticle
Macrophage
Innate response
title_short PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
title_full PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
title_fullStr PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
title_full_unstemmed PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
title_sort PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
author Santos, Diego M.
author_facet Santos, Diego M.
Carneiro, Marcia W.
Moura, Tatiana Rodrigues de
Soto, Manuel
Luz, Nívea Farias
Prates, Deboraci Brito
Barral, Aldina Maria Prado
author_role author
author2 Carneiro, Marcia W.
Moura, Tatiana Rodrigues de
Soto, Manuel
Luz, Nívea Farias
Prates, Deboraci Brito
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Diego M.
Carneiro, Marcia W.
Moura, Tatiana Rodrigues de
Soto, Manuel
Luz, Nívea Farias
Prates, Deboraci Brito
Barral, Aldina Maria Prado
Santos, Diego M.
Carneiro, Marcia W.
Moura, Tatiana Rodrigues de
Soto, Manuel
Luz, Nívea Farias
Prates, Deboraci Brito
Barral, Aldina Maria Prado
dc.subject.por.fl_str_mv Leishmania
PLGA
Nanoparticle
Macrophage
Innate response
topic Leishmania
PLGA
Nanoparticle
Macrophage
Innate response
description Texto completo: acesso restrito. p. 985–995
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-09-09T14:49:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15887
dc.identifier.issn.none.fl_str_mv 1549-9634
dc.identifier.number.pt_BR.fl_str_mv v. 9, n. 7
identifier_str_mv 1549-9634
v. 9, n. 7
url http://repositorio.ufba.br/ri/handle/ri/15887
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.nano.2013.04.003
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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