HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity

Detalhes bibliográficos
Autor(a) principal: Carrara, Vanessa da Silva
Data de Publicação: 2011
Outros Autores: Serra, Lara Zampar, Cardozo Filho, Lúcio, Cunha, Edézio F., Santos, Eduardo C. Torres, Cortez, Diógenes Aparício Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19063
Resumo: Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.
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spelling Carrara, Vanessa da SilvaSerra, Lara ZamparCardozo Filho, LúcioCunha, Edézio F.Santos, Eduardo C. TorresCortez, Diógenes Aparício Garcia2017-05-30T14:45:20Z2017-05-30T14:45:20Z2011CARRARA, Vanessa da Silva; et al. HPLC Analysis of Supercritical Carbon Dioxide and Compressed Propane Extracts from Piper amalago L. with Antileishmanial Activity. Molecules, v.17, p.15-33, 2012.1420-3049https://www.arca.fiocruz.br/handle/icict/1906310.3390/molecules170100151420-3049engMDPIDióxido de carbono supercríticoAlcaloide de pirrolidinaAtividade antileishmanialLeishmaniosePiper amalago L.pyrrolidine alkaloidsupercritical carbon dioxideHPLC validationantileishmanial activityLeishmaniasisHPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.Universidade Estadual de Maringá. Departamento de Engenharia Química. Maringá, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.Piper amalago L. leaves were extracted with supercritical carbon dioxide and compressed propane under different conditions, and with chloroform by the conventional maceration method. These methods were compared for the pyrrolidine alkaloid content. Supercritical carbon dioxide (SFE-CO₂) at 313 K and 12.55 MPa showed the highest selectivity for the main compound (600.53 mg/g of extract). A gradient high-performance liquid chromatography (HPLC) method was developed and validated to quantify the alkaloid N-[7-(3',4'-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl]pyrrolidine in the extracts. The HPLC method showed linearity, precision and accuracy, allowing the quantitative analysis of the alkaloid in all the samples. All the extracts were tested against the promastigote and intracellular amastigote forms of Leishmania amazonensis. The antileishmanial activity was evaluated in terms of inhibitory concentration for 50% of protozoa (IC₅₀). The cytotoxicity was also evaluated against J774A1 macrophages, and the cytotoxic concentrations for 50% of macrophages were obtained (CC₅₀). The SFE-CO₂ (313 K; 12.55 MPa) extract showed the highest antileishmanial activity with the following IC₅₀ values of 16 and 7 µg/mL against the promastigotes and intracellular amastigotes forms, respectively. The extract showed low cytotoxicity with a CC₅₀ value of 93 µg/mL.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19063/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALedezio_cunhajr_etal_IOC_2012.pdfedezio_cunhajr_etal_IOC_2012.pdfapplication/pdf313435https://www.arca.fiocruz.br/bitstream/icict/19063/2/edezio_cunhajr_etal_IOC_2012.pdfe7e4d6a253b6b4d92496c0c610ba155fMD52TEXTedezio_cunhajr_etal_IOC_2012.pdf.txtedezio_cunhajr_etal_IOC_2012.pdf.txtExtracted texttext/plain52978https://www.arca.fiocruz.br/bitstream/icict/19063/3/edezio_cunhajr_etal_IOC_2012.pdf.txt30bd8e04177b154153118d6176ee473eMD53icict/190632018-08-15 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dc.title.pt_BR.fl_str_mv HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
title HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
spellingShingle HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
Carrara, Vanessa da Silva
Dióxido de carbono supercrítico
Alcaloide de pirrolidina
Atividade antileishmanial
Leishmaniose
Piper amalago L.
pyrrolidine alkaloid
supercritical carbon dioxide
HPLC validation
antileishmanial activity
Leishmaniasis
title_short HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
title_full HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
title_fullStr HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
title_full_unstemmed HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
title_sort HPLC analysis of supercritical carbon dioxide and compressed propane extracts from Piper amalago L. with antileishmanial activity
author Carrara, Vanessa da Silva
author_facet Carrara, Vanessa da Silva
Serra, Lara Zampar
Cardozo Filho, Lúcio
Cunha, Edézio F.
Santos, Eduardo C. Torres
Cortez, Diógenes Aparício Garcia
author_role author
author2 Serra, Lara Zampar
Cardozo Filho, Lúcio
Cunha, Edézio F.
Santos, Eduardo C. Torres
Cortez, Diógenes Aparício Garcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carrara, Vanessa da Silva
Serra, Lara Zampar
Cardozo Filho, Lúcio
Cunha, Edézio F.
Santos, Eduardo C. Torres
Cortez, Diógenes Aparício Garcia
dc.subject.other.pt_BR.fl_str_mv Dióxido de carbono supercrítico
Alcaloide de pirrolidina
Atividade antileishmanial
Leishmaniose
topic Dióxido de carbono supercrítico
Alcaloide de pirrolidina
Atividade antileishmanial
Leishmaniose
Piper amalago L.
pyrrolidine alkaloid
supercritical carbon dioxide
HPLC validation
antileishmanial activity
Leishmaniasis
dc.subject.en.pt_BR.fl_str_mv Piper amalago L.
pyrrolidine alkaloid
supercritical carbon dioxide
HPLC validation
antileishmanial activity
Leishmaniasis
description Universidade Estadual de Maringá. Departamento de Farmácia. Maringá, PR, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2017-05-30T14:45:20Z
dc.date.available.fl_str_mv 2017-05-30T14:45:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARRARA, Vanessa da Silva; et al. HPLC Analysis of Supercritical Carbon Dioxide and Compressed Propane Extracts from Piper amalago L. with Antileishmanial Activity. Molecules, v.17, p.15-33, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19063
dc.identifier.issn.pt_BR.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/molecules17010015
dc.identifier.eissn.none.fl_str_mv 1420-3049
identifier_str_mv CARRARA, Vanessa da Silva; et al. HPLC Analysis of Supercritical Carbon Dioxide and Compressed Propane Extracts from Piper amalago L. with Antileishmanial Activity. Molecules, v.17, p.15-33, 2012.
1420-3049
10.3390/molecules17010015
url https://www.arca.fiocruz.br/handle/icict/19063
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/19063/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/19063/2/edezio_cunhajr_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/19063/3/edezio_cunhajr_etal_IOC_2012.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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