Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31367 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. |
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Bottino, Fernanda de OliveiraGardinali, Noemi RovarisSalvador, Sarah Beatrizv SalameneFigueiredo, Andreza SorianoCysne, Lynn BarwickFrancisco, Juliane SiqueiraOliveira, Jaqueline Mendes deMachado, Marcelo PelajoPinto, Marcelo Alves2019-01-31T16:05:31Z2019-01-31T16:05:31Z2018BOTTINO, Fernanda de Oliveira; et al. Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target. Plos One, v.13, n.10, e0205039, 16p, Oct. 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/31367engPublic Library of ScienceVírus da Hepatite ECynomolgus monkeysInfecçãoHepatitis E virusCynomolgus monkeysbone marrownew viral targetInfectionCynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral targetinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Hepatitis E virus (HEV) transmission through infected blood and blood products has already been described. However, little is known about the bone marrow (BM) as source of HEV infection. Our study aimed to investigate the presence of HEV antigen (Ag) and histological changes in BM of cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with HEV. Four cynomolgus monkeys with acute, and two with chronic hepatitis E ─ after immunosuppressive therapy with tacrolimus ─ were compared with one colony-bred animal naturally infected. Both, natural and experimental infections were characterized by anti-HEV IgG seroconversion detected by ELISA, and viral RNA isolation confirmed by RTqPCR and qualitative nested RT-PCR. BM biopsies were collected from all animals, submitted to histology and indirect immunofluorescence techniques and observed, respectively, by light and confocal microscopy. The HEV Ag-fluorescent-labeled cells were detected from BM biopsies obtained from three monkeys with acute and one with chronic hepatitis E, and also from the naturally infected monkey. In the experimentally infected animals with acute hepatitis, HEV Ag detection occurred at 160 days post-infection, even after viral clearance in serum, feces, and liver. Double-stranded RNA, a replicative marker, was detected in BM cells from both acute and chronically infected animals. Major histological findings included vacuolization in mononuclear and endosteal cells, an absence of organized inflammatory infiltrates, and also some fields suggesting displasic focal BM disease. These findings support the hypothesis of BM cells as secondary target sites of HEV persistence. Further experimental studies should be carried out to confirm the assumption of HEV transmission through BM transplantation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31367/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfernandaO_botino_etal_IOC_2018.pdffernandaO_botino_etal_IOC_2018.pdfapplication/pdf36966859https://www.arca.fiocruz.br/bitstream/icict/31367/2/fernandaO_botino_etal_IOC_2018.pdf9ac79249e0096ed02c68ee1dfa19e172MD52TEXTfernandaO_botino_etal_IOC_2018.pdf.txtfernandaO_botino_etal_IOC_2018.pdf.txtExtracted texttext/plain45236https://www.arca.fiocruz.br/bitstream/icict/31367/3/fernandaO_botino_etal_IOC_2018.pdf.txt705f71962b4118f0a86160d5a775b88cMD53icict/313672019-02-01 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| dc.title.pt_BR.fl_str_mv |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| title |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| spellingShingle |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target Bottino, Fernanda de Oliveira Vírus da Hepatite E Cynomolgus monkeys Infecção Hepatitis E virus Cynomolgus monkeys bone marrow new viral target Infection |
| title_short |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| title_full |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| title_fullStr |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| title_full_unstemmed |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| title_sort |
Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target |
| author |
Bottino, Fernanda de Oliveira |
| author_facet |
Bottino, Fernanda de Oliveira Gardinali, Noemi Rovaris Salvador, Sarah Beatrizv Salamene Figueiredo, Andreza Soriano Cysne, Lynn Barwick Francisco, Juliane Siqueira Oliveira, Jaqueline Mendes de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| author_role |
author |
| author2 |
Gardinali, Noemi Rovaris Salvador, Sarah Beatrizv Salamene Figueiredo, Andreza Soriano Cysne, Lynn Barwick Francisco, Juliane Siqueira Oliveira, Jaqueline Mendes de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bottino, Fernanda de Oliveira Gardinali, Noemi Rovaris Salvador, Sarah Beatrizv Salamene Figueiredo, Andreza Soriano Cysne, Lynn Barwick Francisco, Juliane Siqueira Oliveira, Jaqueline Mendes de Machado, Marcelo Pelajo Pinto, Marcelo Alves |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Hepatite E Cynomolgus monkeys Infecção |
| topic |
Vírus da Hepatite E Cynomolgus monkeys Infecção Hepatitis E virus Cynomolgus monkeys bone marrow new viral target Infection |
| dc.subject.en.pt_BR.fl_str_mv |
Hepatitis E virus Cynomolgus monkeys bone marrow new viral target Infection |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil. |
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2018 |
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2018 |
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2019-01-31T16:05:31Z |
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2019-01-31T16:05:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BOTTINO, Fernanda de Oliveira; et al. Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target. Plos One, v.13, n.10, e0205039, 16p, Oct. 2018. |
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https://www.arca.fiocruz.br/handle/icict/31367 |
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1932-6203 |
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BOTTINO, Fernanda de Oliveira; et al. Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target. Plos One, v.13, n.10, e0205039, 16p, Oct. 2018. 1932-6203 |
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