Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus

Bentes, Gentil Arthur; Guimarães, Juliana Rodrigues; Volotão, Eduardo de Mello; Fialho, Alexandre Madi; Hooper, Cleber; Ganime, Ana Carolina; Gardinali, Noemi Rovaris; Lanzarini, Natália Maria; Silva, Alexandre dos Santos da; Pitcovski, Jacob; Leite, José Paulo; Pinto, Marcelo Alves

Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus

Detalhes bibliográficos
Autor(a) principal:	Bentes, Gentil Arthur
Data de Publicação:	2018
Outros Autores:	Guimarães, Juliana Rodrigues, Volotão, Eduardo de Mello, Fialho, Alexandre Madi, Hooper, Cleber, Ganime, Ana Carolina, Gardinali, Noemi Rovaris, Lanzarini, Natália Maria, Silva, Alexandre dos Santos da, Pitcovski, Jacob, Leite, José Paulo, Pinto, Marcelo Alves
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/29461
Resumo:	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.

Metadados do item

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spelling	Bentes, Gentil ArthurGuimarães, Juliana RodriguesVolotão, Eduardo de MelloFialho, Alexandre MadiHooper, CleberGanime, Ana CarolinaGardinali, Noemi RovarisLanzarini, Natália MariaSilva, Alexandre dos Santos daPitcovski, JacobLeite, José PauloPinto, Marcelo Alves2018-10-09T13:52:24Z2018-10-09T13:52:24Z2018BENTES, Gentil Arthur; et al. Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus. Viruses, v.10, n.355, 14p, 2018.1999-4915https://www.arca.fiocruz.br/handle/icict/2946110.3390/v100703551999-4915engMDPIMacaca fascicularismodelo de infecção experimentalrotavírus do grupo A humanohuman group A rotavirusescynomolgus monkeysexperimental infection modelCynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Serviço de Controle da Qualidade Animal. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.MIGAL Technology Center. Virology and Vaccine Development Laboratory. Israel.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Group A rotaviruses (RVA) are one of the most common causes of severe acute gastroenteritis in infants worldwide. Rotaviruses spread from person to person, mainly by faecal⁻oral transmission. Almost all unvaccinated children may become infected with RVA in the first two years of life. The establishment of an experimental monkey model with RVA is important to evaluate new therapeutic approaches. In this study, we demonstrated viral shedding and viraemia in juvenile⁻adult Macaca fascicularis orally inoculated with Wa RVA prototype. Nine monkeys were inoculated orally: seven animals with human RVA and two control animals with saline solution. During the study, the monkeys were clinically monitored, and faeces and blood samples were tested for RVA infection. In general, the inoculated animals developed an oligosymptomatic infection pattern. The main clinical symptoms observed were diarrhoea in two monkeys for three days, associated with a reduction in plasmatic potassium content. Viral RNA was detected in seven faecal and five sera samples from inoculated animals, suggesting virus replication. Cynomolgus monkeys are susceptible hosts for human Wa RVA infection. When inoculated orally, they presented self-limited diarrhoea associated with presence of RVA infectious particles in faeces. Thus, cynomolgus monkeys may be useful as animal models to evaluate the efficacy of new antiviral approaches.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29461/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgentil_bentes_etal_IOC_2018.pdfgentil_bentes_etal_IOC_2018.pdfapplication/pdf3661954https://www.arca.fiocruz.br/bitstream/icict/29461/2/gentil_bentes_etal_IOC_2018.pdf45163caeab19a76f53c50788c263f240MD52TEXTgentil_bentes_etal_IOC_2018.pdf.txtgentil_bentes_etal_IOC_2018.pdf.txtExtracted texttext/plain56572https://www.arca.fiocruz.br/bitstream/icict/29461/3/gentil_bentes_etal_IOC_2018.pdf.txt3eaf30201e2b22185e01d131eeda6c1bMD53icict/294612018-10-10 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dc.title.pt_BR.fl_str_mv	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
title	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
spellingShingle	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus Bentes, Gentil Arthur Macaca fascicularis modelo de infecção experimental rotavírus do grupo A humano human group A rotaviruses cynomolgus monkeys experimental infection model
title_short	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
title_full	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
title_fullStr	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
title_full_unstemmed	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
title_sort	Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus
author	Bentes, Gentil Arthur
author_facet	Bentes, Gentil Arthur Guimarães, Juliana Rodrigues Volotão, Eduardo de Mello Fialho, Alexandre Madi Hooper, Cleber Ganime, Ana Carolina Gardinali, Noemi Rovaris Lanzarini, Natália Maria Silva, Alexandre dos Santos da Pitcovski, Jacob Leite, José Paulo Pinto, Marcelo Alves
author_role	author
author2	Guimarães, Juliana Rodrigues Volotão, Eduardo de Mello Fialho, Alexandre Madi Hooper, Cleber Ganime, Ana Carolina Gardinali, Noemi Rovaris Lanzarini, Natália Maria Silva, Alexandre dos Santos da Pitcovski, Jacob Leite, José Paulo Pinto, Marcelo Alves
author2_role	author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Bentes, Gentil Arthur Guimarães, Juliana Rodrigues Volotão, Eduardo de Mello Fialho, Alexandre Madi Hooper, Cleber Ganime, Ana Carolina Gardinali, Noemi Rovaris Lanzarini, Natália Maria Silva, Alexandre dos Santos da Pitcovski, Jacob Leite, José Paulo Pinto, Marcelo Alves
dc.subject.other.pt_BR.fl_str_mv	Macaca fascicularis modelo de infecção experimental rotavírus do grupo A humano
topic	Macaca fascicularis modelo de infecção experimental rotavírus do grupo A humano human group A rotaviruses cynomolgus monkeys experimental infection model
dc.subject.en.pt_BR.fl_str_mv	human group A rotaviruses cynomolgus monkeys experimental infection model
description	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.
publishDate	2018
dc.date.accessioned.fl_str_mv	2018-10-09T13:52:24Z
dc.date.available.fl_str_mv	2018-10-09T13:52:24Z
dc.date.issued.fl_str_mv	2018
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	BENTES, Gentil Arthur; et al. Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus. Viruses, v.10, n.355, 14p, 2018.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/29461
dc.identifier.issn.pt_BR.fl_str_mv	1999-4915
dc.identifier.doi.none.fl_str_mv	10.3390/v10070355
dc.identifier.eissn.none.fl_str_mv	1999-4915
identifier_str_mv	BENTES, Gentil Arthur; et al. Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus. Viruses, v.10, n.355, 14p, 2018. 1999-4915 10.3390/v10070355
url	https://www.arca.fiocruz.br/handle/icict/29461
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/29461/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/29461/2/gentil_bentes_etal_IOC_2018.pdf https://www.arca.fiocruz.br/bitstream/icict/29461/3/gentil_bentes_etal_IOC_2018.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
_version_	1813008964418273280

Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus

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