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spelling Golden, AllisonOliveira-Silva, MichelleSlater, HannahVieira, Alexia MartinesBansil, PoojaGerth-Guyette, EmilyLeader, Brandon T.Zobrist, StephanieFerreira, Alan Kennedy BragaAraúo, Erika Crhistina Santos deCruz, Catherine Duran de LucenaGarbin, EduardoBizilj, Greg T.Carlson, Sean J.Sagalovsky, MarianaPal, SampaGupta, VinWolansky, LeoBoyle, David S.Dall’Acqua, Deusilene Souza VieiraNaveca, Felipe GomesNascimento, Valdinete Alves doSalcedo, Juan Miguel VillalobosDrain, Paul K.Costa, Alexandre Dias TavaresPereira, DhélioDomingo, Gonzalo J.2023-08-23T15:46:06Z2023-08-23T15:46:06Z2023GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023.1932-6203https://www.arca.fiocruz.br/handle/icict/60199https://doi.org/10.1371/ journal.pone.0287814porPublic Library of ScienceAntigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen typesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDiagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Diagnostics. PATH. Seattle, Washington, USA.Amazon.com. Seattle, Washington, USA.The Rockefeller Foundation. Pandemic Prevention Institute. New York, USA.Diagnostics. PATH. Seattle, Washington, USA.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Department of Global Health. University of Washington. Seattle, WA, USA. / Department of Medicine. University of Washington. Seattle, WA, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas, Curitiba, PR, Brasil.Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Diagnostics. PATH. Seattle, Washington, USA.The relationship between N-antigen concentration and viral load within and across different specimens guides the clinical performance of rapid diagnostic tests (RDT) in different uses. A prospective study was conducted in Porto Velho, Brazil, to investigate RDT performance in different specimen types as a function of the correlation between antigen concentration and viral load. The study included 214 close contacts with recent exposures to confirmed cases, aged 12 years and older and with various levels of vaccination. Antigen concentration was measured in nasopharyngeal swab (NPS), anterior nares swab (ANS), and saliva specimens. Reverse transcriptase (RT)–PCR was conducted on the NPS and saliva specimens, and two RDTs were conducted on ANS and one RDT on saliva. Antigen concentration correlated well with viral load when measured in the same specimen type but not across specimen types. Antigen levels were higher in symptomatic cases compared to asymptomatic/oligosymptomatic cases and lower in saliva compared to NPS and ANS samples. Discordant results between the RDTs conducted on ANS and the RT-PCR on NPS were resolved by antigen concentration values. The analytical limit of detection of RDTs can be used to predict the performance of the tests in populations for which the antigen concentration is known. The antigen dynamics across different sample types observed in SARS-CoV-2 disease progression support use of RDTs with nasal samples. Given lower antigen concentrations in saliva, rapid testing using saliva is expected to require improved RDT analytical sensitivity to achieve clinical sensitivity similar to rapid testing of nasal samples.Rapid Diagnostic TestsSampling StudiesAntigensPrueba de Diagnóstico RápidoMuestreoAntígenosÉtudes par échantillonnageAntigènesSARS-CoV-2Testes de Diagnóstico RápidoAmostragemAntígenosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60199/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjournal.pone.Antigenok.pdfjournal.pone.Antigenok.pdfapplication/pdf2534735https://www.arca.fiocruz.br/bitstream/icict/60199/2/journal.pone.Antigenok.pdfbd961651729bbd0fefc1a7d2411db3fbMD52icict/601992023-08-23 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dc.title.en_US.fl_str_mv Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
title Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
spellingShingle Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
Golden, Allison
Rapid Diagnostic Tests
Sampling Studies
Antigens
Prueba de Diagnóstico Rápido
Muestreo
Antígenos
Études par échantillonnage
Antigènes
SARS-CoV-2
Testes de Diagnóstico Rápido
Amostragem
Antígenos
title_short Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
title_full Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
title_fullStr Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
title_full_unstemmed Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
title_sort Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types
author Golden, Allison
author_facet Golden, Allison
Oliveira-Silva, Michelle
Slater, Hannah
Vieira, Alexia Martines
Bansil, Pooja
Gerth-Guyette, Emily
Leader, Brandon T.
Zobrist, Stephanie
Ferreira, Alan Kennedy Braga
Araúo, Erika Crhistina Santos de
Cruz, Catherine Duran de Lucena
Garbin, Eduardo
Bizilj, Greg T.
Carlson, Sean J.
Sagalovsky, Mariana
Pal, Sampa
Gupta, Vin
Wolansky, Leo
Boyle, David S.
Dall’Acqua, Deusilene Souza Vieira
Naveca, Felipe Gomes
Nascimento, Valdinete Alves do
Salcedo, Juan Miguel Villalobos
Drain, Paul K.
Costa, Alexandre Dias Tavares
Pereira, Dhélio
Domingo, Gonzalo J.
author_role author
author2 Oliveira-Silva, Michelle
Slater, Hannah
Vieira, Alexia Martines
Bansil, Pooja
Gerth-Guyette, Emily
Leader, Brandon T.
Zobrist, Stephanie
Ferreira, Alan Kennedy Braga
Araúo, Erika Crhistina Santos de
Cruz, Catherine Duran de Lucena
Garbin, Eduardo
Bizilj, Greg T.
Carlson, Sean J.
Sagalovsky, Mariana
Pal, Sampa
Gupta, Vin
Wolansky, Leo
Boyle, David S.
Dall’Acqua, Deusilene Souza Vieira
Naveca, Felipe Gomes
Nascimento, Valdinete Alves do
Salcedo, Juan Miguel Villalobos
Drain, Paul K.
Costa, Alexandre Dias Tavares
Pereira, Dhélio
Domingo, Gonzalo J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Golden, Allison
Oliveira-Silva, Michelle
Slater, Hannah
Vieira, Alexia Martines
Bansil, Pooja
Gerth-Guyette, Emily
Leader, Brandon T.
Zobrist, Stephanie
Ferreira, Alan Kennedy Braga
Araúo, Erika Crhistina Santos de
Cruz, Catherine Duran de Lucena
Garbin, Eduardo
Bizilj, Greg T.
Carlson, Sean J.
Sagalovsky, Mariana
Pal, Sampa
Gupta, Vin
Wolansky, Leo
Boyle, David S.
Dall’Acqua, Deusilene Souza Vieira
Naveca, Felipe Gomes
Nascimento, Valdinete Alves do
Salcedo, Juan Miguel Villalobos
Drain, Paul K.
Costa, Alexandre Dias Tavares
Pereira, Dhélio
Domingo, Gonzalo J.
dc.subject.en.en_US.fl_str_mv Rapid Diagnostic Tests
Sampling Studies
Antigens
topic Rapid Diagnostic Tests
Sampling Studies
Antigens
Prueba de Diagnóstico Rápido
Muestreo
Antígenos
Études par échantillonnage
Antigènes
SARS-CoV-2
Testes de Diagnóstico Rápido
Amostragem
Antígenos
dc.subject.es.en_US.fl_str_mv Prueba de Diagnóstico Rápido
Muestreo
Antígenos
dc.subject.fr.en_US.fl_str_mv Études par échantillonnage
Antigènes
dc.subject.decs.en_US.fl_str_mv SARS-CoV-2
Testes de Diagnóstico Rápido
Amostragem
Antígenos
description Diagnostics. PATH. Seattle, Washington, USA.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-08-23T15:46:06Z
dc.date.available.fl_str_mv 2023-08-23T15:46:06Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60199
dc.identifier.issn.en_US.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/ journal.pone.0287814
identifier_str_mv GOLDEN, Allison et al. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types. PLoS ONE, v. 18, n. 7, p. 1 -13, 2023.
1932-6203
url https://www.arca.fiocruz.br/handle/icict/60199
https://doi.org/10.1371/ journal.pone.0287814
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dc.publisher.none.fl_str_mv Public Library of Science
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