Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3389/fimmu.2018.01223 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28468 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. |
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Barbosa, Mayara Garcia de MattosSilva, Bruno Jorge de AndradeAssis, Tayná QuintellaPrata, Rhana Berto da SilvaFerreira, HelenAndrade, Priscila RibeiroOliveira, Jéssica Araújo da Paixão deSilva, Gilberto Marcelo Sperandio daNery, José Augusto da CostaSarno, Euzenir NunesPinheiro, Roberta Olmo2018-08-30T13:48:56Z2018-08-30T13:48:56Z2018BARBOSA, Mayara Garcia de Mattos; et al. Autophagy impairment is associated with increased inflammasome activation and reversal reaction development im multibacillary leprosy. Frontiers in Immunology, v.9, Article 1223, 14p, June 2018.1664-3224https://www.arca.fiocruz.br/handle/icict/2846810.3389/fimmu.2018.01223engFrontiers MediaHanseníaseMycobacterium lepraeAutofagiapacientes multibacilaresreação reversainflamassomaLeprosymultibacillary patientsreversal reactionautophagyinflammasomeMycobacterium lepraeAutophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil.Leprosy reactions are responsible for incapacities in leprosy and represent the major cause of permanent neuropathy. The identification of biomarkers able to identify patients more prone to develop reaction could contribute to adequate clinical management and the prevention of disability. Reversal reaction may occur in unstable borderline patients and also in lepromatous patients. To identify biomarker signature profiles related with the reversal reaction onset, multibacillary patients were recruited and classified accordingly the occurrence or not of reversal reaction during or after multidrugtherapy. Analysis of skin lesion cells at diagnosis of multibacillary leprosy demonstrated that in the group that developed reaction (T1R) in the future there was a downregulation of autophagy associated with the overexpression of TLR2 and MLST8. The autophagy impairment in T1R group was associated with increased expression of NLRP3, caspase-1 (p10) and IL-1β production. In addition, analysis of IL-1β production in serum from multibacillary patients demonstrated that patients who developed reversal reaction have significantly increased concentrations of IL-1β at diagnosis, suggesting that the pattern of innate immune responses could predict the reactional episode outcome. In vitro analysis demonstrated that the blockade of autophagy with 3-methyladenine (3-MA) in Mycobacterium leprae-stimulated human primary monocytes increased the assembly of NLRP3 specks assembly, and it was associated with an increase of IL-1β and IL-6 production. Together, our data suggest an important role for autophagy in multibacillary leprosy patients to avoid exacerbated inflammasome activation and the onset of reversal reaction.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28468/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmayara_barbosa_etal_IOC_2018.pdfmayara_barbosa_etal_IOC_2018.pdfapplication/pdf5575468https://www.arca.fiocruz.br/bitstream/icict/28468/2/mayara_barbosa_etal_IOC_2018.pdf7acb0915ac111905a61ad9a888557353MD52TEXTmayara_barbosa_etal_IOC_2018.pdf.txtmayara_barbosa_etal_IOC_2018.pdf.txtExtracted texttext/plain69019https://www.arca.fiocruz.br/bitstream/icict/28468/3/mayara_barbosa_etal_IOC_2018.pdf.txt72267f25e95eaff351c11befb2c9c44eMD53icict/284682022-06-24 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| dc.title.pt_BR.fl_str_mv |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| title |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| spellingShingle |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy Barbosa, Mayara Garcia de Mattos Hanseníase Mycobacterium leprae Autofagia pacientes multibacilares reação reversa inflamassoma Leprosy multibacillary patients reversal reaction autophagy inflammasome Mycobacterium leprae |
| title_short |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| title_full |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| title_fullStr |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| title_full_unstemmed |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| title_sort |
Autophagy Impairment Is Associated With Increased Inflammasome Activation and Reversal Reaction Development in Multibacillary Leprosy |
| author |
Barbosa, Mayara Garcia de Mattos |
| author_facet |
Barbosa, Mayara Garcia de Mattos Silva, Bruno Jorge de Andrade Assis, Tayná Quintella Prata, Rhana Berto da Silva Ferreira, Helen Andrade, Priscila Ribeiro Oliveira, Jéssica Araújo da Paixão de Silva, Gilberto Marcelo Sperandio da Nery, José Augusto da Costa Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| author_role |
author |
| author2 |
Silva, Bruno Jorge de Andrade Assis, Tayná Quintella Prata, Rhana Berto da Silva Ferreira, Helen Andrade, Priscila Ribeiro Oliveira, Jéssica Araújo da Paixão de Silva, Gilberto Marcelo Sperandio da Nery, José Augusto da Costa Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barbosa, Mayara Garcia de Mattos Silva, Bruno Jorge de Andrade Assis, Tayná Quintella Prata, Rhana Berto da Silva Ferreira, Helen Andrade, Priscila Ribeiro Oliveira, Jéssica Araújo da Paixão de Silva, Gilberto Marcelo Sperandio da Nery, José Augusto da Costa Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| dc.subject.other.pt_BR.fl_str_mv |
Hanseníase Mycobacterium leprae Autofagia pacientes multibacilares reação reversa inflamassoma |
| topic |
Hanseníase Mycobacterium leprae Autofagia pacientes multibacilares reação reversa inflamassoma Leprosy multibacillary patients reversal reaction autophagy inflammasome Mycobacterium leprae |
| dc.subject.en.pt_BR.fl_str_mv |
Leprosy multibacillary patients reversal reaction autophagy inflammasome Mycobacterium leprae |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ. Brasil. |
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2018 |
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2018-08-30T13:48:56Z |
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2018-08-30T13:48:56Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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BARBOSA, Mayara Garcia de Mattos; et al. Autophagy impairment is associated with increased inflammasome activation and reversal reaction development im multibacillary leprosy. Frontiers in Immunology, v.9, Article 1223, 14p, June 2018. |
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https://www.arca.fiocruz.br/handle/icict/28468 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2018.01223 |
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BARBOSA, Mayara Garcia de Mattos; et al. Autophagy impairment is associated with increased inflammasome activation and reversal reaction development im multibacillary leprosy. Frontiers in Immunology, v.9, Article 1223, 14p, June 2018. 1664-3224 10.3389/fimmu.2018.01223 |
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