Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.

Detalhes bibliográficos
Autor(a) principal: Andrade, Luiza Freire
Data de Publicação: 2011
Outros Autores: Nahum, Laila Alves, Avelar, Livia das Graças Amaral, Silva, Larissa Lopes, Zerlotini, Adhemar, Conceição, Ruiz Jerônimo, Oliveira, Guilherme Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8101
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil
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spelling Andrade, Luiza FreireNahum, Laila AlvesAvelar, Livia das Graças AmaralSilva, Larissa LopesZerlotini, AdhemarConceição, Ruiz JerônimoOliveira, Guilherme Corrêa de2014-07-31T11:09:14Z2014-07-31T11:09:14Z2011ANDRADE, Luiza Freire et al. Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni. BMC Genomics 12: 215, 2011.1471-2164https://www.arca.fiocruz.br/handle/icict/8101engBiomedcentralEukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Excelência em Bioinformática. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Excelência em Bioinformática. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Excelência em Bioinformática. Belo Horizonte, MG, BrazilBACKGROUND: Schistosomiasis remains an important parasitic disease and a major economic problem in many countries. The Schistosoma mansoni genome and predicted proteome sequences were recently published providing the opportunity to identify new drug candidates. Eukaryotic protein kinases (ePKs) play a central role in mediating signal transduction through complex networks and are considered druggable targets from the medical and chemical viewpoints. Our work aimed at analyzing the S. mansoni predicted proteome in order to identify and classify all ePKs of this parasite through combined computational approaches. Functional annotation was performed mainly to yield insights into the parasite signaling processes relevant to its complex lifestyle and to select some ePKs as potential drug targets. RESULTS: We have identified 252 ePKs, which corresponds to 1.9% of the S. mansoni predicted proteome, through sequence similarity searches using HMMs (Hidden Markov Models). Amino acid sequences corresponding to the conserved catalytic domain of ePKs were aligned by MAFFT and further used in distance-based phylogenetic analysis as implemented in PHYLIP. Our analysis also included the ePK homologs from six other eukaryotes. The results show that S. mansoni has proteins in all ePK groups. Most of them are clearly clustered with known ePKs in other eukaryotes according to the phylogenetic analysis. None of the ePKs are exclusively found in S. mansoni or belong to an expanded family in this parasite. Only 16 S. mansoni ePKs were experimentally studied, 12 proteins are predicted to be catalytically inactive and approximately 2% of the parasite ePKs remain unclassified. Some proteins were mentioned as good target for drug development since they have a predicted essential function for the parasite. CONCLUSIONS: Our approach has improved the functional annotation of 40% of S. mansoni ePKs through combined similarity and phylogenetic-based approaches. As we continue this work, we will highlight the biochemical and physiological adaptations of S. mansoni in response to diverse environments during the parasite development, vector interaction, and host infection.Protein Kinases/chemistryProteome/metabolismSchistosoma mansoni/cytologySignal Transductioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALEukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.pdfEukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.pdfapplication/pdf4374199https://www.arca.fiocruz.br/bitstream/icict/8101/1/Eukaryotic%20protein%20kinases%20%28ePKs%29%20of%20the%20helminth%20parasite%20Schistosoma%20mansoni.pdf9dc18529787c603ef883bdb239790003MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8101/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTEukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.pdf.txtEukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.pdf.txtExtracted texttext/plain19https://www.arca.fiocruz.br/bitstream/icict/8101/3/Eukaryotic%20protein%20kinases%20%28ePKs%29%20of%20the%20helminth%20parasite%20Schistosoma%20mansoni.pdf.txt7f5b903a193cc66524e06d8c0458e34aMD53icict/81012018-04-06 08:43:57.714oai:www.arca.fiocruz.br:icict/8101TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
title Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
spellingShingle Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
Andrade, Luiza Freire
Protein Kinases/chemistry
Proteome/metabolism
Schistosoma mansoni/cytology
Signal Transduction
title_short Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
title_full Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
title_fullStr Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
title_full_unstemmed Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
title_sort Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni.
author Andrade, Luiza Freire
author_facet Andrade, Luiza Freire
Nahum, Laila Alves
Avelar, Livia das Graças Amaral
Silva, Larissa Lopes
Zerlotini, Adhemar
Conceição, Ruiz Jerônimo
Oliveira, Guilherme Corrêa de
author_role author
author2 Nahum, Laila Alves
Avelar, Livia das Graças Amaral
Silva, Larissa Lopes
Zerlotini, Adhemar
Conceição, Ruiz Jerônimo
Oliveira, Guilherme Corrêa de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Luiza Freire
Nahum, Laila Alves
Avelar, Livia das Graças Amaral
Silva, Larissa Lopes
Zerlotini, Adhemar
Conceição, Ruiz Jerônimo
Oliveira, Guilherme Corrêa de
dc.subject.en.pt_BR.fl_str_mv Protein Kinases/chemistry
Proteome/metabolism
Schistosoma mansoni/cytology
Signal Transduction
topic Protein Kinases/chemistry
Proteome/metabolism
Schistosoma mansoni/cytology
Signal Transduction
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Genomics and Computational Biology Group. Belo Horizonte, MG, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-07-31T11:09:14Z
dc.date.available.fl_str_mv 2014-07-31T11:09:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, Luiza Freire et al. Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni. BMC Genomics 12: 215, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8101
dc.identifier.issn.none.fl_str_mv 1471-2164
identifier_str_mv ANDRADE, Luiza Freire et al. Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni. BMC Genomics 12: 215, 2011.
1471-2164
url https://www.arca.fiocruz.br/handle/icict/8101
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Biomedcentral
publisher.none.fl_str_mv Biomedcentral
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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