Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment

Detalhes bibliográficos
Autor(a) principal: González, Florencia Belén
Data de Publicação: 2016
Outros Autores: Calmon-Hamaty, Flavia, Cordeiro, Synara Nô Seara, Bussy, Rodrigo Fernández, Spinelli, Silvana Virginia, D'Attilio, Luciano, Bottasso, Oscar, Savino, Wilson, Almeida, Vinícius Cotta de, Villar, Silvina Raquel, Pérez, Ana Rosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41051
Resumo: Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
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spelling González, Florencia BelénCalmon-Hamaty, FlaviaCordeiro, Synara Nô SearaBussy, Rodrigo FernándezSpinelli, Silvana VirginiaD'Attilio, LucianoBottasso, OscarSavino, WilsonAlmeida, Vinícius Cotta deVillar, Silvina RaquelPérez, Ana Rosa2020-04-30T18:41:30Z2020-04-30T18:41:30Z2016GONZÁLEZ, Florencia Belén et al. Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. Plos Neglect Tropical Diseases, v. 10, n. 1, p. 1-21, Jan. 2018.1935-2727https://www.arca.fiocruz.br/handle/icict/4105110.1371/journal.pntd.00042851935-2735engPublic Library of ScienceTrypanosoma cruziImpactoCélulas T reguladoras do timoInfecção experimentalTrypanosoma cruziExperimental InfectionThymic Regulatory T Cell CompartmentImpactsTrypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (α chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41051/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALViniciusCAlmeida_FCalmonHamaty_etal_IOC_2016.pdfViniciusCAlmeida_FCalmonHamaty_etal_IOC_2016.pdfapplication/pdf5133320https://www.arca.fiocruz.br/bitstream/icict/41051/2/ViniciusCAlmeida_FCalmonHamaty_etal_IOC_2016.pdfa6aaf45a2f2abd27b2b86088477f8646MD52TEXTViniciusCAlmeida_FCalmonHamaty_etal_IOC_2016.pdf.txtViniciusCAlmeida_FCalmonHamaty_etal_IOC_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
spellingShingle Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
González, Florencia Belén
Trypanosoma cruzi
Impacto
Células T reguladoras do timo
Infecção experimental
Trypanosoma cruzi
Experimental Infection
Thymic Regulatory T Cell Compartment
Impacts
title_short Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_full Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_fullStr Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_full_unstemmed Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
title_sort Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment
author González, Florencia Belén
author_facet González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Bussy, Rodrigo Fernández
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Almeida, Vinícius Cotta de
Villar, Silvina Raquel
Pérez, Ana Rosa
author_role author
author2 Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Bussy, Rodrigo Fernández
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Almeida, Vinícius Cotta de
Villar, Silvina Raquel
Pérez, Ana Rosa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv González, Florencia Belén
Calmon-Hamaty, Flavia
Cordeiro, Synara Nô Seara
Bussy, Rodrigo Fernández
Spinelli, Silvana Virginia
D'Attilio, Luciano
Bottasso, Oscar
Savino, Wilson
Almeida, Vinícius Cotta de
Villar, Silvina Raquel
Pérez, Ana Rosa
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Impacto
Células T reguladoras do timo
Infecção experimental
topic Trypanosoma cruzi
Impacto
Células T reguladoras do timo
Infecção experimental
Trypanosoma cruzi
Experimental Infection
Thymic Regulatory T Cell Compartment
Impacts
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Experimental Infection
Thymic Regulatory T Cell Compartment
Impacts
description Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR). Rosario, Argentina.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2020-04-30T18:41:30Z
dc.date.available.fl_str_mv 2020-04-30T18:41:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONZÁLEZ, Florencia Belén et al. Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. Plos Neglect Tropical Diseases, v. 10, n. 1, p. 1-21, Jan. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41051
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004285
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv GONZÁLEZ, Florencia Belén et al. Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment. Plos Neglect Tropical Diseases, v. 10, n. 1, p. 1-21, Jan. 2018.
1935-2727
10.1371/journal.pntd.0004285
1935-2735
url https://www.arca.fiocruz.br/handle/icict/41051
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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