Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis

Detalhes bibliográficos
Autor(a) principal: Nunes, Marise P.
Data de Publicação: 1992
Outros Autores: Sobral, Ana Cristina L., Coutinho, Sérgio G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43489
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
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spelling Nunes, Marise P.Sobral, Ana Cristina L.Coutinho, Sérgio G.2020-09-20T20:46:47Z2020-09-20T20:46:47Z1992NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992.0074-0276https://www.arca.fiocruz.br/handle/icict/4348910.1590/S0074-027619920004000071678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziInfecção experimentalLimitando a análise de diluiçãoTrypanosoma cruziLimiting dilution analysisExperimental infectionQuantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Limiting dilution analysis was used to quantify Trypanosoma cruzi in the lymph nodes, liver and heart of Swiss and C57 B1/10 mice. The results showed that, in Swiss and B1/10 mice infected with T. cruzi Y strain, the number of parasites/mg of tissue increased during the course of the infection in both types of mice, although a grater number of parasites were observed in heart tissue from Swiss mice than from B1/10. With regard to liver tissue, it was observed that the parasite load in the initial phase of infection was higher than in heart. In experiments using T. cruzi Colombian strain, the parasite load in the heart of Swiss and B1/10 mice increased relatively slowly, although high levels of parasitization were nonetheless observable by the end of the infection. As for the liver and lymph nodes, the concentration of parasites was lower over the entire course of infection than in heart. Both strains thus maintained their characteristic tissue tropisms. The limiting dilution assay (LDA) proved to be an appropriate method for more precise quantification of T. cruzi, comparing favorably with other direct microscopic methods that only give approximate scores.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43489/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariseP_Nunes_etal_IOC_1992.pdfMariseP_Nunes_etal_IOC_1992.pdfapplication/pdf314072https://www.arca.fiocruz.br/bitstream/icict/43489/2/MariseP_Nunes_etal_IOC_1992.pdf6316e4679c503f1d020f10dad1d95391MD52TEXTMariseP_Nunes_etal_IOC_1992.pdf.txtMariseP_Nunes_etal_IOC_1992.pdf.txtExtracted texttext/plain7https://www.arca.fiocruz.br/bitstream/icict/43489/3/MariseP_Nunes_etal_IOC_1992.pdf.txt212b0306580d4f0044d18f9a3edcc832MD53icict/434892020-09-21 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dc.title.pt_BR.fl_str_mv Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
title Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
spellingShingle Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
Nunes, Marise P.
Trypanosoma cruzi
Infecção experimental
Limitando a análise de diluição
Trypanosoma cruzi
Limiting dilution analysis
Experimental infection
title_short Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
title_full Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
title_fullStr Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
title_full_unstemmed Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
title_sort Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
author Nunes, Marise P.
author_facet Nunes, Marise P.
Sobral, Ana Cristina L.
Coutinho, Sérgio G.
author_role author
author2 Sobral, Ana Cristina L.
Coutinho, Sérgio G.
author2_role author
author
dc.contributor.author.fl_str_mv Nunes, Marise P.
Sobral, Ana Cristina L.
Coutinho, Sérgio G.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Infecção experimental
Limitando a análise de diluição
topic Trypanosoma cruzi
Infecção experimental
Limitando a análise de diluição
Trypanosoma cruzi
Limiting dilution analysis
Experimental infection
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Limiting dilution analysis
Experimental infection
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
publishDate 1992
dc.date.issued.fl_str_mv 1992
dc.date.accessioned.fl_str_mv 2020-09-20T20:46:47Z
dc.date.available.fl_str_mv 2020-09-20T20:46:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43489
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761992000400007
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992.
0074-0276
10.1590/S0074-02761992000400007
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43489
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/43489/3/MariseP_Nunes_etal_IOC_1992.pdf.txt
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