TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells

Detalhes bibliográficos
Autor(a) principal: Pérez, Ana Rosa
Data de Publicação: 2012
Outros Autores: Berbert, Luiz Ricardo, Lepletier, Ailin, Revelli, Silvia, Bottasso, Oscar, Barbosa, Suse Dayse Silva, Savino, Wilson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18498
Resumo: National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.
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spelling Pérez, Ana RosaBerbert, Luiz RicardoLepletier, AilinRevelli, SilviaBottasso, OscarBarbosa, Suse Dayse SilvaSavino, Wilson2017-04-18T16:27:15Z2017-04-18T16:27:15Z2012PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1849810.1371/journal.pone.0034360engPublic Library of ScienceTrypanosoma cruziMigração anormal de timócitosInfecção experimentalCélulas imaturasDoença de ChagasTNF-aTrypanosoma cruziAbnormal Thymocyte MigrationChagas DiseaseExperimental InfectionTNF-aImmature CellsTNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental Chagas disease. Migratory activity of thymocytes and mature T cells seem to be finely tuned by cytokines, chemokines and extracellular matrix (ECM) components. Systemic TNF-α is enhanced during infection and appears to be crucial in the response against the parasite. However, it also seems to be involved in disease pathology, since it is implicated in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-α in the migratory activity of thymocytes in Trypanosoma cruzi (T. cruzi) acutely-infected mice. We found increased expression and deposition of TNF-α in the thymus of infected animals compared to controls, accompanied by increased co-localization of fibronectin, a cell migration-related ECM molecule, whose contents in the thymus of infected mice is also augmented. In-vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-α. The increase of immature CD4(+)CD8(+) T cells in secondary lymphoid organs was even more clear-cut when TNF-α was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-α was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4(+)CD8(+). Infected animals also exhibit enhanced levels of expression of both mRNA TNF-α receptors in the CD4(+)CD8(+) subpopulation. Our findings suggest that in T. cruzi acute infection, when TNF-α is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-α is a further player in the process.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18498/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALailin_lepletier_etal_IOC_2012.pdfailin_lepletier_etal_IOC_2012.pdfapplication/pdf1212603https://www.arca.fiocruz.br/bitstream/icict/18498/2/ailin_lepletier_etal_IOC_2012.pdf81521811fdd9e8b406d1af6327687af3MD52TEXTailin_lepletier_etal_IOC_2012.pdf.txtailin_lepletier_etal_IOC_2012.pdf.txtExtracted texttext/plain52018https://www.arca.fiocruz.br/bitstream/icict/18498/3/ailin_lepletier_etal_IOC_2012.pdf.txtd17b63ea36f3a62f55cdde468cae724aMD53icict/184982018-08-15 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dc.title.pt_BR.fl_str_mv TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
title TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
spellingShingle TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
Pérez, Ana Rosa
Trypanosoma cruzi
Migração anormal de timócitos
Infecção experimental
Células imaturas
Doença de Chagas
TNF-a
Trypanosoma cruzi
Abnormal Thymocyte Migration
Chagas Disease
Experimental Infection
TNF-a
Immature Cells
title_short TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
title_full TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
title_fullStr TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
title_full_unstemmed TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
title_sort TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
author Pérez, Ana Rosa
author_facet Pérez, Ana Rosa
Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia
Bottasso, Oscar
Barbosa, Suse Dayse Silva
Savino, Wilson
author_role author
author2 Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia
Bottasso, Oscar
Barbosa, Suse Dayse Silva
Savino, Wilson
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pérez, Ana Rosa
Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia
Bottasso, Oscar
Barbosa, Suse Dayse Silva
Savino, Wilson
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Migração anormal de timócitos
Infecção experimental
Células imaturas
Doença de Chagas
TNF-a
topic Trypanosoma cruzi
Migração anormal de timócitos
Infecção experimental
Células imaturas
Doença de Chagas
TNF-a
Trypanosoma cruzi
Abnormal Thymocyte Migration
Chagas Disease
Experimental Infection
TNF-a
Immature Cells
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Abnormal Thymocyte Migration
Chagas Disease
Experimental Infection
TNF-a
Immature Cells
description National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-04-18T16:27:15Z
dc.date.available.fl_str_mv 2017-04-18T16:27:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18498
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0034360
identifier_str_mv PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012.
1932-6203
10.1371/journal.pone.0034360
url https://www.arca.fiocruz.br/handle/icict/18498
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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