TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18498 |
Resumo: | National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina. |
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Pérez, Ana RosaBerbert, Luiz RicardoLepletier, AilinRevelli, SilviaBottasso, OscarBarbosa, Suse Dayse SilvaSavino, Wilson2017-04-18T16:27:15Z2017-04-18T16:27:15Z2012PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1849810.1371/journal.pone.0034360engPublic Library of ScienceTrypanosoma cruziMigração anormal de timócitosInfecção experimentalCélulas imaturasDoença de ChagasTNF-aTrypanosoma cruziAbnormal Thymocyte MigrationChagas DiseaseExperimental InfectionTNF-aImmature CellsTNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental Chagas disease. Migratory activity of thymocytes and mature T cells seem to be finely tuned by cytokines, chemokines and extracellular matrix (ECM) components. Systemic TNF-α is enhanced during infection and appears to be crucial in the response against the parasite. However, it also seems to be involved in disease pathology, since it is implicated in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-α in the migratory activity of thymocytes in Trypanosoma cruzi (T. cruzi) acutely-infected mice. We found increased expression and deposition of TNF-α in the thymus of infected animals compared to controls, accompanied by increased co-localization of fibronectin, a cell migration-related ECM molecule, whose contents in the thymus of infected mice is also augmented. In-vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-α. The increase of immature CD4(+)CD8(+) T cells in secondary lymphoid organs was even more clear-cut when TNF-α was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-α was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4(+)CD8(+). Infected animals also exhibit enhanced levels of expression of both mRNA TNF-α receptors in the CD4(+)CD8(+) subpopulation. Our findings suggest that in T. cruzi acute infection, when TNF-α is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-α is a further player in the process.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18498/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALailin_lepletier_etal_IOC_2012.pdfailin_lepletier_etal_IOC_2012.pdfapplication/pdf1212603https://www.arca.fiocruz.br/bitstream/icict/18498/2/ailin_lepletier_etal_IOC_2012.pdf81521811fdd9e8b406d1af6327687af3MD52TEXTailin_lepletier_etal_IOC_2012.pdf.txtailin_lepletier_etal_IOC_2012.pdf.txtExtracted texttext/plain52018https://www.arca.fiocruz.br/bitstream/icict/18498/3/ailin_lepletier_etal_IOC_2012.pdf.txtd17b63ea36f3a62f55cdde468cae724aMD53icict/184982018-08-15 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| dc.title.pt_BR.fl_str_mv |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| title |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| spellingShingle |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells Pérez, Ana Rosa Trypanosoma cruzi Migração anormal de timócitos Infecção experimental Células imaturas Doença de Chagas TNF-a Trypanosoma cruzi Abnormal Thymocyte Migration Chagas Disease Experimental Infection TNF-a Immature Cells |
| title_short |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| title_full |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| title_fullStr |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| title_full_unstemmed |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| title_sort |
TNF-α is involved in the abnormal thymocyte migration during experimental Trypanosoma cruzi infection and favors the export of immature cells |
| author |
Pérez, Ana Rosa |
| author_facet |
Pérez, Ana Rosa Berbert, Luiz Ricardo Lepletier, Ailin Revelli, Silvia Bottasso, Oscar Barbosa, Suse Dayse Silva Savino, Wilson |
| author_role |
author |
| author2 |
Berbert, Luiz Ricardo Lepletier, Ailin Revelli, Silvia Bottasso, Oscar Barbosa, Suse Dayse Silva Savino, Wilson |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Pérez, Ana Rosa Berbert, Luiz Ricardo Lepletier, Ailin Revelli, Silvia Bottasso, Oscar Barbosa, Suse Dayse Silva Savino, Wilson |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Migração anormal de timócitos Infecção experimental Células imaturas Doença de Chagas TNF-a |
| topic |
Trypanosoma cruzi Migração anormal de timócitos Infecção experimental Células imaturas Doença de Chagas TNF-a Trypanosoma cruzi Abnormal Thymocyte Migration Chagas Disease Experimental Infection TNF-a Immature Cells |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Abnormal Thymocyte Migration Chagas Disease Experimental Infection TNF-a Immature Cells |
| description |
National University of Rosario. Faculty of Medical Sciences. Institute of Immunology. Rosario, Argentina. |
| publishDate |
2012 |
| dc.date.issued.fl_str_mv |
2012 |
| dc.date.accessioned.fl_str_mv |
2017-04-18T16:27:15Z |
| dc.date.available.fl_str_mv |
2017-04-18T16:27:15Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012. |
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https://www.arca.fiocruz.br/handle/icict/18498 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0034360 |
| identifier_str_mv |
PÉREZ, Ana Rosa; et al. TNF-a Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells. Plos One, v.7, n.3, 10p, Mar. 2012. 1932-6203 10.1371/journal.pone.0034360 |
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