Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11013 |
Resumo: | CAPES |
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Santos, Fabio BritoBarbosa, Glaucia GonçalvesTrilles, LucianaNishikawa, Marília MartinsWanke, BodoMeyer, WielandCosta, Filipe Anibal CarvalhoLazera, Márcia dos Santos2015-06-30T13:58:10Z2015-06-30T13:58:10Z2015SANTOS, Fabio Brito et al. Environmental isolation of Cryptococcus gattii VGII from indoor dust from typical wooden houses in the deep Amazonas of the Rio Negro basin. PLOS One, v. 10, n. 2, p. 1-11, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1101310.1371/journal.pone.0115866CAPESFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School-Westmead Hospital. New South Wales, Australia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia Molecular e Sistemática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Cryptococcosis is a human fungal infection of significant mortality and morbidity, especially in the meningoencephalitis form. Cryptococcosis is distributed worldwide and its agents, C. neoformans and C. gattii, present eight major molecular types—VNI-VNIV and VGI-VGIV respectively. The primary cryptococcosis caused by molecular type VGII (serotype B, MATalpha) prevails in immunocompetent patients in the North and Northeast of Brazil, revealing an endemic regional pattern to this molecular type. Since 1999, C. gattii VGII has been involved in an ongoing outbreak in Canada, and is expanding to the Northwest of the United States, two temperate regions. Exposure to propagules dispersed in the environment, related to various organic substrates, mainly decomposing wood in and around dwellings, initiates the infection process. The present study investigated the presence of the agents of crypto-coccos is in dust from dwellings in the upper Rio Negro, municipality of Santa Isabel do Rio Negro in Amazonas state. Indoor dust was collected from 51 houses, diluted and plated on bird seed agar. Dark brown colonies were identified phenotypically, and genotypically byURA5 restriction fragment length polymorphism analysis and multilocus sequence typing(MLST). The mating type was identified using pheromone-specific primers. Three of the 51houses were positive for C. gattii molecular type VGII,MATα and MATa, showing a high prevalence of this agent. MLST studies identified eight subtypes, VGIIb (ST7), VGIIa (ST20),(ST5) and 5 new subtypes unique to the region. For the first time in the state of Amazonas, C. gattii VGIIMATα and MATa were isolated from the environment and correlates with endemic cryptococcosis in this state. This is the first description of MLST subtypes on environ-mental isolates in the Brazilian Amazon, indicating domiciliary dust as a potential source for human infection with different subtypes of C. gattii VGIIMATα and MATa.engPLOS OneFungosCryptococcus gattiiTécnicas de Tipagem MicológicaMicologiaFungiCryptococcus gattiiMycological Typing TechniquesMycologyFungosCryptococcus gattiiTécnicas de Tipagem MicológicaMicologiaEnvironmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11013/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALPLoS_One_10_2_e0115866.pdfPLoS_One_10_2_e0115866.pdfapplication/pdf1152096https://www.arca.fiocruz.br/bitstream/icict/11013/2/PLoS_One_10_2_e0115866.pdf5dc7c4c1b3ae2be3bd38f38944bd8022MD52TEXTPLoS_One_10_2_e0115866.pdf.txtPLoS_One_10_2_e0115866.pdf.txtExtracted texttext/plain37839https://www.arca.fiocruz.br/bitstream/icict/11013/3/PLoS_One_10_2_e0115866.pdf.txt442850b9b650b45b45a766d18738e078MD53icict/110132023-09-14 15:46:16.101oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:46:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en.fl_str_mv |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| title |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| spellingShingle |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin Santos, Fabio Brito Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia Fungi Cryptococcus gattii Mycological Typing Techniques Mycology Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia |
| title_short |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| title_full |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| title_fullStr |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| title_full_unstemmed |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| title_sort |
Environmental Isolation of VGII from Indoor Dust from Typical Wooden Houses in the Deep Amazonas of the Rio Negro Basin |
| author |
Santos, Fabio Brito |
| author_facet |
Santos, Fabio Brito Barbosa, Glaucia Gonçalves Trilles, Luciana Nishikawa, Marília Martins Wanke, Bodo Meyer, Wieland Costa, Filipe Anibal Carvalho Lazera, Márcia dos Santos |
| author_role |
author |
| author2 |
Barbosa, Glaucia Gonçalves Trilles, Luciana Nishikawa, Marília Martins Wanke, Bodo Meyer, Wieland Costa, Filipe Anibal Carvalho Lazera, Márcia dos Santos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Fabio Brito Barbosa, Glaucia Gonçalves Trilles, Luciana Nishikawa, Marília Martins Wanke, Bodo Meyer, Wieland Costa, Filipe Anibal Carvalho Lazera, Márcia dos Santos |
| dc.subject.other.pt_BR.fl_str_mv |
Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia |
| topic |
Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia Fungi Cryptococcus gattii Mycological Typing Techniques Mycology Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia |
| dc.subject.en.en.fl_str_mv |
Fungi Cryptococcus gattii Mycological Typing Techniques Mycology |
| dc.subject.decs.pt_BR.fl_str_mv |
Fungos Cryptococcus gattii Técnicas de Tipagem Micológica Micologia |
| description |
CAPES |
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2015 |
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2015-06-30T13:58:10Z |
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2015-06-30T13:58:10Z |
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2015 |
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info:eu-repo/semantics/article |
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SANTOS, Fabio Brito et al. Environmental isolation of Cryptococcus gattii VGII from indoor dust from typical wooden houses in the deep Amazonas of the Rio Negro basin. PLOS One, v. 10, n. 2, p. 1-11, 2015. |
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https://www.arca.fiocruz.br/handle/icict/11013 |
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1932-6203 |
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10.1371/journal.pone.0115866 |
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SANTOS, Fabio Brito et al. Environmental isolation of Cryptococcus gattii VGII from indoor dust from typical wooden houses in the deep Amazonas of the Rio Negro basin. PLOS One, v. 10, n. 2, p. 1-11, 2015. 1932-6203 10.1371/journal.pone.0115866 |
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