Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy

Detalhes bibliográficos
Autor(a) principal: Couto-Fernandez, J. C.
Data de Publicação: 2005
Outros Autores: Silva-de-Jesus, C., Veloso, Valdiléa G., Rachid, M., Gracie, R. S. G., Chequer-Fernandez, S. L., Oliveira, S. M., Arakaki-Sanchez, D., Chequer, P. J. N., Morgado, M. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57590
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Couto-Fernandez, J. C.Silva-de-Jesus, C.Veloso, Valdiléa G.Rachid, M.Gracie, R. S. G.Chequer-Fernandez, S. L.Oliveira, S. M.Arakaki-Sanchez, D.Chequer, P. J. N.Morgado, M. G.2023-03-27T14:39:09Z2023-03-27T14:39:09Z2005COUTO-FERNANDEZ, J. C. et al. HIV-1 Genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated iutations in HIV-1 infected individuals failing the highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005.0074-0276https://www.arca.fiocruz.br/handle/icict/5759010.1590/S0074-027620050001000141678-8060engFiocruz/IOCHuman immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Secretaria Estadual de Saúde do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. DIS/CIC. Laboratório de Geoprocessamento. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.Ministério da Saúde. Programa Nacional de DST e AIDS. Brasília, DF, Brasil.In order to assess the human immunodeficiency virus type 1 (HIV-1) drug resistance mutation profiles and evaluate the distribution of the genetic subtypes in the state of Rio de Janeiro, Brazil, blood samples from 547 HIV-1 infected patients failing antiretroviral (ARV) therapy, were collected during the years 2002 and 2003 to perform the viral resistance genotyping at the Renageno Laboratory from Rio de Janeiro (Oswaldo Cruz Foundation). Viral resistance genotyping was performed using ViroSeqTM Genotyping System (Celera Diagnostic-Abbott, US). The HIV-1 subtyping based on polymerase (pol) gene sequences (protease and reverse transcriptase-RT regions) was as follows: subtype B (91.2%), subtype F (4.9%), and B/F viral recombinant forms (3.3%). The subtype C was identified in two patients (0.4%) and the recombinant CRF_02/AG virus was found infecting one patient (0.2%). The HIV-1 genotyping profile associated to the reverse transcriptase inhibitors has shown a high frequency of the M184V mutation followed by the timidine-associated mutations. The K103N mutation was the most prevalent to the non-nucleoside RT inhibitor and the resistance associated to protease inhibitor showed the minor mutations L63P, L10F/R, and A71V as the more prevalent. A large proportion of subtype B was observed in HIV-1 treated patients from Rio de Janeiro. In addition, we have identified the circulation of drug-resistant HIV-1 subtype C and are presenting the first report of the occurrence of an African recombinant CRF_02/AG virus in Rio de Janeiro, Brazil. A clear association between HIV-1 subtypes and protease resistance mutations was observed in this study. The maintenance of resistance genotyping programs for HIV-1 failing patients is important to the management of ARV therapies and to attempt and monitor the HIV-1 subtype prevalence in Brazil.Human immunodeficiency virus type 1SubtypesGenotypingResistanceBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Veloso_V_G_INI_2005.pdfve_Veloso_V_G_INI_2005.pdfapplication/pdf565096https://www.arca.fiocruz.br/bitstream/icict/57590/2/ve_Veloso_V_G_INI_2005.pdfb48e343e53af68bf5d3ee93a3ddfdb39MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57590/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/575902023-09-04 11:30:21.075oai:www.arca.fiocruz.br:icict/57590Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:30:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
title Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
spellingShingle Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
Couto-Fernandez, J. C.
Human immunodeficiency virus type 1
Subtypes
Genotyping
Resistance
Brazil
title_short Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
title_full Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
title_fullStr Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
title_full_unstemmed Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
title_sort Human immunodeficiency virus type 1 HIV-1 genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated mutations in HIV-1 infected individuals failing the highly active antiretroviral therapy
author Couto-Fernandez, J. C.
author_facet Couto-Fernandez, J. C.
Silva-de-Jesus, C.
Veloso, Valdiléa G.
Rachid, M.
Gracie, R. S. G.
Chequer-Fernandez, S. L.
Oliveira, S. M.
Arakaki-Sanchez, D.
Chequer, P. J. N.
Morgado, M. G.
author_role author
author2 Silva-de-Jesus, C.
Veloso, Valdiléa G.
Rachid, M.
Gracie, R. S. G.
Chequer-Fernandez, S. L.
Oliveira, S. M.
Arakaki-Sanchez, D.
Chequer, P. J. N.
Morgado, M. G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Couto-Fernandez, J. C.
Silva-de-Jesus, C.
Veloso, Valdiléa G.
Rachid, M.
Gracie, R. S. G.
Chequer-Fernandez, S. L.
Oliveira, S. M.
Arakaki-Sanchez, D.
Chequer, P. J. N.
Morgado, M. G.
dc.subject.en.en_US.fl_str_mv Human immunodeficiency virus type 1
Subtypes
Genotyping
Resistance
Brazil
topic Human immunodeficiency virus type 1
Subtypes
Genotyping
Resistance
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2023-03-27T14:39:09Z
dc.date.available.fl_str_mv 2023-03-27T14:39:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COUTO-FERNANDEZ, J. C. et al. HIV-1 Genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated iutations in HIV-1 infected individuals failing the highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57590
dc.identifier.issn.en_US.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762005000100014
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv COUTO-FERNANDEZ, J. C. et al. HIV-1 Genotyping in Rio de Janeiro, Brazil: assessing subtype and drug-resistance associated iutations in HIV-1 infected individuals failing the highly active antiretroviral therapy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 1, p. 73-78, 2005.
0074-0276
10.1590/S0074-02762005000100014
1678-8060
url https://www.arca.fiocruz.br/handle/icict/57590
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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