Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans

Detalhes bibliográficos
Autor(a) principal: Rizzo, Juliana
Data de Publicação: 2014
Outros Autores: Oliveira, Débora L., Joffe, Luna S., Hu, Guanggan, Gazos-Lopes, Felipe, Fonseca, Fernanda Lopes, Almeida, Igor C., Frases, Susana, Kronstad, James W., Rodrigues, Marcio Lourenço
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31993
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
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spelling Rizzo, JulianaOliveira, Débora L.Joffe, Luna S.Hu, GuangganGazos-Lopes, FelipeFonseca, Fernanda LopesAlmeida, Igor C.Frases, SusanaKronstad, James W.Rodrigues, Marcio Lourenço2019-03-07T20:40:52Z2019-03-07T20:40:52Z2014RIZZO, J. et al. Role of the Apt1 Protein in Polysaccharide Secretion by Cryptococcus neoformans. Eukaryotic Cell, v. 13, n. 6, p. 715–726, 2014.1535-9778https://www.arca.fiocruz.br/handle/icict/3199310.1128/EC.00273-13engAmerican Society of MicrobiologyRole of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.The University of British Columbia. Faculty of Land and Food Systems. Department of Microbiology and Immunology. Michael Smith Laboratories. Vancouver, Canada.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.The University of British Columbia. Faculty of Land and Food Systems. Department of Microbiology and Immunology. Michael Smith Laboratories. Vancouver, Canada.The University of Texas at El Paso. Department of Biological Sciences. Border Biomedical Research Center. El Paso, Texas, USA.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.The University of Texas at El Paso. Department of Biological Sciences. Border Biomedical Research Center. El Paso, Texas, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, BrazilThe University of British Columbia. Faculty of Land and Food Systems. Department of Microbiology and Immunology. Michael Smith Laboratories. Vancouver, Canada.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Flippases are key regulators of membrane asymmetry and secretory mechanisms. Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans. On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant. Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties. The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells. Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus. These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization. This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain. Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.Apt1 ProteinPolysaccharide SecretionCryptococcus neoformansinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/31993/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Rodrigues_etal_CDTS_2013f.pdfve_Marcio_Rodrigues_etal_CDTS_2013f.pdfapplication/pdf1895669https://www.arca.fiocruz.br/bitstream/icict/31993/2/ve_Marcio_Rodrigues_etal_CDTS_2013f.pdf6dea90228d254715230bf0e6f7e4def6MD52TEXTve_Marcio_Rodrigues_etal_CDTS_2013f.pdf.txtve_Marcio_Rodrigues_etal_CDTS_2013f.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
title Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
spellingShingle Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
Rizzo, Juliana
Apt1 Protein
Polysaccharide Secretion
Cryptococcus neoformans
title_short Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
title_full Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
title_fullStr Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
title_full_unstemmed Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
title_sort Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans
author Rizzo, Juliana
author_facet Rizzo, Juliana
Oliveira, Débora L.
Joffe, Luna S.
Hu, Guanggan
Gazos-Lopes, Felipe
Fonseca, Fernanda Lopes
Almeida, Igor C.
Frases, Susana
Kronstad, James W.
Rodrigues, Marcio Lourenço
author_role author
author2 Oliveira, Débora L.
Joffe, Luna S.
Hu, Guanggan
Gazos-Lopes, Felipe
Fonseca, Fernanda Lopes
Almeida, Igor C.
Frases, Susana
Kronstad, James W.
Rodrigues, Marcio Lourenço
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rizzo, Juliana
Oliveira, Débora L.
Joffe, Luna S.
Hu, Guanggan
Gazos-Lopes, Felipe
Fonseca, Fernanda Lopes
Almeida, Igor C.
Frases, Susana
Kronstad, James W.
Rodrigues, Marcio Lourenço
dc.subject.en.pt_BR.fl_str_mv Apt1 Protein
Polysaccharide Secretion
Cryptococcus neoformans
topic Apt1 Protein
Polysaccharide Secretion
Cryptococcus neoformans
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2019-03-07T20:40:52Z
dc.date.available.fl_str_mv 2019-03-07T20:40:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIZZO, J. et al. Role of the Apt1 Protein in Polysaccharide Secretion by Cryptococcus neoformans. Eukaryotic Cell, v. 13, n. 6, p. 715–726, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31993
dc.identifier.issn.pt_BR.fl_str_mv 1535-9778
dc.identifier.doi.none.fl_str_mv 10.1128/EC.00273-13
identifier_str_mv RIZZO, J. et al. Role of the Apt1 Protein in Polysaccharide Secretion by Cryptococcus neoformans. Eukaryotic Cell, v. 13, n. 6, p. 715–726, 2014.
1535-9778
10.1128/EC.00273-13
url https://www.arca.fiocruz.br/handle/icict/31993
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Microbiology
publisher.none.fl_str_mv American Society of Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31993/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31993/2/ve_Marcio_Rodrigues_etal_CDTS_2013f.pdf
https://www.arca.fiocruz.br/bitstream/icict/31993/3/ve_Marcio_Rodrigues_etal_CDTS_2013f.pdf.txt
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