Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues

Detalhes bibliográficos
Autor(a) principal: Motoie, Gabriela
Data de Publicação: 2013
Outros Autores: Ferreira, Gabriel Eduardo Melim, Cupolillo, Elisa, Canavez, Flavio, Chioccola, Vera Lucia Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12682
Resumo: Instituto Adolfo Lutz. Laboratório de Parasitologia. São Paulo, SP, Brasil.
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spelling Motoie, GabrielaFerreira, Gabriel Eduardo MelimCupolillo, ElisaCanavez, FlavioChioccola, Vera Lucia Pereira2016-02-02T14:30:10Z2016-02-02T14:30:10Z2013MOTOIE, Gabriela; et al. Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues. Infection, Genetics and Evolution, n.18, p.48–59, Aug. 2013.1567-1348https://www.arca.fiocruz.br/handle/icict/1268210.1016/j.meegid.2013.04.0311567-7257engElsevierSpatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissuesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Adolfo Lutz. Laboratório de Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Genoa Biotecnologia SA. São Paulo, SP, Brasil.Instituto Adolfo Lutz. Laboratório de Parasitologia. São Paulo, SP, Brasil.This study investigated the genetic characteristics of Leishmania infantum samples from São Paulo (SP) State, Brazil in order to collaborate with information about the possible origins of the parasites, as well as, the introduction and spread of visceral leishmaniasis in this Brazilian State. Multilocus microsatellite typing (MLMT) was performed using a set of 17 microsatellite markers. DNA was extracted from 250 samples collected from dogs diagnosed with visceral leishmaniasis and 112 (45%) were genotyped: 67 from the northwest region (NWSP), and 29 from the southeast region (SESP) of SP. The results were correlated with other 16 samples from Mato Grosso do Sul State (MS) (which borders NWSP). Although, a small portion of samples was genotyped, it was possible to genotype multiple loci using small amounts of Leishmania DNA extracted directly from dog tissues. Despite the fact that MLMT analysis defined 33 different genotypes, a low polymorphism was detected within the parasites studied with 10 polymorphic loci. There are two main genetic clusters circulating in SP with strong genetic differentiation, one (POP-A) is composed by samples from SESP and NWSP and presented a weak signal of geographical substructure. The other, belongs to the same cluster found in the state of MS (POP-B), which was the main one. The majority (93.75%) of MS parasite genotypes belonged to POP-B, with just one sample (6.25%) grouped in POP-A. POP-B also comprised 10.34% of SESP and 26.87% of NWSP samples. Besides one sample from MS, POP-A is composed by 73.13% of NWSP and 89.66% of SESP samples. The MLMT analysis supported the idea of canine visceral leishmaniasis being introduced in the Northwest region of SP State by the traf- fic of humans and dogs from MS. In the southeast region of SP occurred an introduction of a new L. infantum genetic cluster. Probably the transmission was spread by traffic of infected dogs from other Brazilian regions, or by introduction of imported dogs from other countries. All these data together contributed to the detection of the genetic profile of L. infantum population in SP State.Leishmania infantumMicrosatellite markersAmerican visceral leishmaniasisClinical samplesSão Paulo State – BrazilRepetições de MicrossatélitesLeishmania infantumLeishmaniose Visceralinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12682/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALelisa2_cupolillo_etal_IOC_2013.pdfelisa2_cupolillo_etal_IOC_2013.pdfapplication/pdf1792242https://www.arca.fiocruz.br/bitstream/icict/12682/2/elisa2_cupolillo_etal_IOC_2013.pdf2fda69c728d8e94a91346a5c0da5a1f3MD52TEXTelisa2_cupolillo_etal_IOC_2013.pdf.txtelisa2_cupolillo_etal_IOC_2013.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
title Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
spellingShingle Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
Motoie, Gabriela
Leishmania infantum
Microsatellite markers
American visceral leishmaniasis
Clinical samples
São Paulo State – Brazil
Repetições de Microssatélites
Leishmania infantum
Leishmaniose Visceral
title_short Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
title_full Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
title_fullStr Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
title_full_unstemmed Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
title_sort Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues
author Motoie, Gabriela
author_facet Motoie, Gabriela
Ferreira, Gabriel Eduardo Melim
Cupolillo, Elisa
Canavez, Flavio
Chioccola, Vera Lucia Pereira
author_role author
author2 Ferreira, Gabriel Eduardo Melim
Cupolillo, Elisa
Canavez, Flavio
Chioccola, Vera Lucia Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Motoie, Gabriela
Ferreira, Gabriel Eduardo Melim
Cupolillo, Elisa
Canavez, Flavio
Chioccola, Vera Lucia Pereira
dc.subject.en.pt_BR.fl_str_mv Leishmania infantum
Microsatellite markers
American visceral leishmaniasis
Clinical samples
São Paulo State – Brazil
topic Leishmania infantum
Microsatellite markers
American visceral leishmaniasis
Clinical samples
São Paulo State – Brazil
Repetições de Microssatélites
Leishmania infantum
Leishmaniose Visceral
dc.subject.decs.pt_BR.fl_str_mv Repetições de Microssatélites
Leishmania infantum
Leishmaniose Visceral
description Instituto Adolfo Lutz. Laboratório de Parasitologia. São Paulo, SP, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-02-02T14:30:10Z
dc.date.available.fl_str_mv 2016-02-02T14:30:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOTOIE, Gabriela; et al. Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues. Infection, Genetics and Evolution, n.18, p.48–59, Aug. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12682
dc.identifier.issn.none.fl_str_mv 1567-1348
dc.identifier.doi.none.fl_str_mv 10.1016/j.meegid.2013.04.031
dc.identifier.eissn.none.fl_str_mv 1567-7257
identifier_str_mv MOTOIE, Gabriela; et al. Spatial distribution and population genetics of Leishmania infantum genotypes in São Paulo State, Brazil, employingmultilocus microsatellite typing directly in dog infected tissues. Infection, Genetics and Evolution, n.18, p.48–59, Aug. 2013.
1567-1348
10.1016/j.meegid.2013.04.031
1567-7257
url https://www.arca.fiocruz.br/handle/icict/12682
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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