Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production

Detalhes bibliográficos
Autor(a) principal: Paiva, Ligia Almeida
Data de Publicação: 2015
Outros Autores: Coelho, Karen Almeida, Gomes, Tatiana Luna, El-Cheikh, Marcia Cury, Borojevic, Radovan, Perez, Sandra Aurora, Bozza, Patrícia Torres, Melo, Christianne Bandeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12108
Resumo: 2016-03-31
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spelling Paiva, Ligia AlmeidaCoelho, Karen AlmeidaGomes, Tatiana LunaEl-Cheikh, Marcia CuryBorojevic, RadovanPerez, Sandra AuroraBozza, Patrícia TorresMelo, Christianne Bandeira2015-11-03T14:46:52Z2016-04-01T07:30:09Z2015PAIVA, Ligia Almeida; et al. Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), v.95, p.57-62, Apr. 2015.0952-3278https://www.arca.fiocruz.br/handle/icict/1210810.1016/j.plefa.2015.01.004engElsevierSchistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-03-31Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Hepatic Stellate Cells (HSCs) play a crucial role in pathogenesis of liver inflammation and fibrosis. During chronic liver injury, HSCs lose vitamin A and transform into myofibroblastic cells. In schistosomal granulomas, these activated HSCs are called GR-HSCs. Schistosomal-triggered hepatic fibrogenesis has TGF-β as the most potent fibrogenic stimulus, that also controls gene expression of the angiogenic molecule VEGF in HSCs. COX-dependent production of prostaglandins (PGs) also play role in angiogenic processes. Besides angiogenic roles, prostanoids control immunomodulation of Schistosoma mansoni infection. Specifically, schistosoma-derived PGD2 has emerged as a key parasite regulator of immune defense evasion, while no role is still established to host PGD2. Therefore, the aim of this work is to investigate the ability of GR-HSCs to synthesize COX-derived PGD2 and a potential role of this prostanoid in VEGF production by GR-HSCs in vitro. Here, we confirmed that GR-HSCs express COX-2, which displayed perinuclear localization. While unstimulated GR-HSCs produce basal levels of PGD2, TGF-β stimulation besides increasing COX2- mRNA levels, enhanced synthesis/secretion of PGD2 in GR-HSCs supernatant. Moreover, GR-HSCs-derived PGD2 mediate VEGF production by TGF-β-stimulated GR-HSCs, since the pre-treatment with HQL-79, an inhibitor of hematopoietic PGD synthase inhibited both PGD2 synthesis and VEGF secretion by TGF-β-stimulated GR-HSCs. All together, our findings show an autocrine/paracrine activity of GR-HSCs-derived PGD2 on TGF-β-induced VEGF production by GR-HSCs, unveiling a role for PGD2 as important regulator of HSCs activation in hepatic granulomas from schistosome infected mice.Hepatic Stellate CellsProstaglandin D2S. mansoniVEGFEosinophilCélulas Estreladas do FígadoProstaglandina D2EosinófiloSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12108/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALligia_paiva_etal_IOC_2015.pdfligia_paiva_etal_IOC_2015.pdfapplication/pdf1438560https://www.arca.fiocruz.br/bitstream/icict/12108/2/ligia_paiva_etal_IOC_2015.pdf15842b82374b7fc67e531eafbfc7ef3bMD52TEXTligia_paiva_etal_IOC_2015.pdf.txtligia_paiva_etal_IOC_2015.pdf.txtExtracted texttext/plain36657https://www.arca.fiocruz.br/bitstream/icict/12108/3/ligia_paiva_etal_IOC_2015.pdf.txte4083559b3d041587afa35b2146b333cMD53icict/121082023-09-05 10:31:03.953oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:31:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
title Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
spellingShingle Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
Paiva, Ligia Almeida
Hepatic Stellate Cells
Prostaglandin D2
S. mansoni
VEGF
Eosinophil
Células Estreladas do Fígado
Prostaglandina D2
Eosinófilo
Schistosoma mansoni
title_short Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
title_full Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
title_fullStr Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
title_full_unstemmed Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
title_sort Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production
author Paiva, Ligia Almeida
author_facet Paiva, Ligia Almeida
Coelho, Karen Almeida
Gomes, Tatiana Luna
El-Cheikh, Marcia Cury
Borojevic, Radovan
Perez, Sandra Aurora
Bozza, Patrícia Torres
Melo, Christianne Bandeira
author_role author
author2 Coelho, Karen Almeida
Gomes, Tatiana Luna
El-Cheikh, Marcia Cury
Borojevic, Radovan
Perez, Sandra Aurora
Bozza, Patrícia Torres
Melo, Christianne Bandeira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paiva, Ligia Almeida
Coelho, Karen Almeida
Gomes, Tatiana Luna
El-Cheikh, Marcia Cury
Borojevic, Radovan
Perez, Sandra Aurora
Bozza, Patrícia Torres
Melo, Christianne Bandeira
dc.subject.en.pt_BR.fl_str_mv Hepatic Stellate Cells
Prostaglandin D2
S. mansoni
VEGF
Eosinophil
topic Hepatic Stellate Cells
Prostaglandin D2
S. mansoni
VEGF
Eosinophil
Células Estreladas do Fígado
Prostaglandina D2
Eosinófilo
Schistosoma mansoni
dc.subject.decs.pt_BR.fl_str_mv Células Estreladas do Fígado
Prostaglandina D2
Eosinófilo
Schistosoma mansoni
description 2016-03-31
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-11-03T14:46:52Z
dc.date.issued.fl_str_mv 2015
dc.date.available.fl_str_mv 2016-04-01T07:30:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAIVA, Ligia Almeida; et al. Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), v.95, p.57-62, Apr. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12108
dc.identifier.issn.none.fl_str_mv 0952-3278
dc.identifier.doi.none.fl_str_mv 10.1016/j.plefa.2015.01.004
identifier_str_mv PAIVA, Ligia Almeida; et al. Schistosome infection-derived Hepatic Stellate Cells are cellular source of prostaglandin D2: Role in TGF-β-stimulated VEGF production. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), v.95, p.57-62, Apr. 2015.
0952-3278
10.1016/j.plefa.2015.01.004
url https://www.arca.fiocruz.br/handle/icict/12108
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12108/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12108/2/ligia_paiva_etal_IOC_2015.pdf
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