Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds

Detalhes bibliográficos
Autor(a) principal: Grangeiro, Thalles Barbosa
Data de Publicação: 1997
Outros Autores: Schriefer, Albert, Calvete, Juan J, Raida, Manfred, Urbanke, Claus, Barral Netto, Manoel, Cavada, Benildo Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7449
Resumo: Universidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, Brasil
id CRUZ_a37168cdd30e9aa9f86c62a9df9db3c4
oai_identifier_str oai:www.arca.fiocruz.br:icict/7449
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Grangeiro, Thalles BarbosaSchriefer, AlbertCalvete, Juan JRaida, ManfredUrbanke, ClausBarral Netto, ManoelCavada, Benildo Sousa2014-03-26T14:05:44Z2014-03-26T14:05:44Z1997GRANGEIRO, T. B. et al. Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds. Eur.opean Journal of Biochemistry, v. 248, n. 1, p. 43-48, 1997.0014-2956https://www.arca.fiocruz.br/handle/icict/7449engSpringer VerlagMolecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seedsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilInstitut fur Reproduktionsmedizin. Tierarztliche Hochschule. Hannover, GermanyNiedersachsisches Institut fur Peptid-Forschung GmbH. Hannover, GermanyBiophysikalische MeRgerateabteilung. Medizinische Hochschule. Hannover, GermanyFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, BrasilConBr, a lectin isolated from Canavalia brusiliensis seeds, shares with other legume plant lectins from the genus Canavaliu (Diocleinae subtribe) primary carbohydrate recognition specificity for D-mannose and D-glucose. However, ConBr exerts different biological effects than concanavalin A, the lectin of Canavalia ensiformis seeds, regarding induction of rat paw edema, peritoneal macrophage spreading in mouse, and in vitro human lymphocyte stimulation. The primary structure of ConBr was established by cDNA cloning, amino acid sequencing, and mass spectrometry. The 237-amino-acid sequence of ConBr displays Ser/Thr heterogeneity at position 96, indicating the existence of two isoforms. The mature Canavalia brasiliensis lectin monomer consists of a mixture of predominantly full-length polypeptide (a-chain) and a small proportion of fragments 1-118 (J-chain) and 119-237 (y-chain). Although ConBr isolectins and concanavalin A differ only in residues at positions 58, 70, and 96, ConBr monomers associate into dimers and tetramers in a different pH-dependent manner than those of concanavalin A. The occurrence of glycine at position 58 does not allow formation of the hydrogen bond that in the concanavalin A tetramer exists between Asp58 of subunit A and Sec62 of subunit C. The consequence is that the a carbons of the corresponding residues in ConBr are 1.5 A closer that in concanavalin A, and ConBr adopts a more open quaternary structure than concanavalin A. Our data support the hypothesis that substitution of amino acids located at the subunit interface of structurally related lectins of the same protein family can lead to different quaternary conformations that may account for their different biological activities.Legume lectinCanavalia brasiliensisNucleotide sequenceAmino acid sequence ; mass spectrometryFabaceae/genéticaLectinas/genéticaPlantas MedicinaisSequência de AminoácidosAnimaisSequência de BasesSítios de LigaçãoClonagem MolecularPrimers do DNA/genéticaEdema/etiologiaGenes de PlantasLigações de HidrogênioHumanosConcentração de Íons de HidrogênioModelos MolecularesReação em Cadeia da Polimeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGrangeiro TB MOlecular cloning....pdfGrangeiro TB MOlecular cloning....pdfapplication/pdf804349https://www.arca.fiocruz.br/bitstream/icict/7449/1/Grangeiro%20TB%20MOlecular%20cloning....pdf38c947d41b2ca3da027419d0ca6b500cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7449/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTGrangeiro TB MOlecular cloning....pdf.txtGrangeiro TB MOlecular cloning....pdf.txtExtracted texttext/plain37558https://www.arca.fiocruz.br/bitstream/icict/7449/5/Grangeiro%20TB%20MOlecular%20cloning....pdf.txt537c6758b7133a7489ef38786832fa51MD55THUMBNAILGrangeiro TB MOlecular cloning....pdf.jpgGrangeiro TB MOlecular cloning....pdf.jpgGenerated Thumbnailimage/jpeg2301https://www.arca.fiocruz.br/bitstream/icict/7449/4/Grangeiro%20TB%20MOlecular%20cloning....pdf.jpgcf760a710ade33d7567befb3b8ada128MD54icict/74492023-03-15 14:33:22.467oai:www.arca.fiocruz.br:icict/7449TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
title Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
spellingShingle Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
Grangeiro, Thalles Barbosa
Legume lectin
Canavalia brasiliensis
Nucleotide sequence
Amino acid sequence ; mass spectrometry
Fabaceae/genética
Lectinas/genética
Plantas Medicinais
Sequência de Aminoácidos
Animais
Sequência de Bases
Sítios de Ligação
Clonagem Molecular
Primers do DNA/genética
Edema/etiologia
Genes de Plantas
Ligações de Hidrogênio
Humanos
Concentração de Íons de Hidrogênio
Modelos Moleculares
Reação em Cadeia da Polimerase
title_short Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
title_full Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
title_fullStr Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
title_full_unstemmed Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
title_sort Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds
author Grangeiro, Thalles Barbosa
author_facet Grangeiro, Thalles Barbosa
Schriefer, Albert
Calvete, Juan J
Raida, Manfred
Urbanke, Claus
Barral Netto, Manoel
Cavada, Benildo Sousa
author_role author
author2 Schriefer, Albert
Calvete, Juan J
Raida, Manfred
Urbanke, Claus
Barral Netto, Manoel
Cavada, Benildo Sousa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Grangeiro, Thalles Barbosa
Schriefer, Albert
Calvete, Juan J
Raida, Manfred
Urbanke, Claus
Barral Netto, Manoel
Cavada, Benildo Sousa
dc.subject.en.pt_BR.fl_str_mv Legume lectin
Canavalia brasiliensis
Nucleotide sequence
Amino acid sequence ; mass spectrometry
topic Legume lectin
Canavalia brasiliensis
Nucleotide sequence
Amino acid sequence ; mass spectrometry
Fabaceae/genética
Lectinas/genética
Plantas Medicinais
Sequência de Aminoácidos
Animais
Sequência de Bases
Sítios de Ligação
Clonagem Molecular
Primers do DNA/genética
Edema/etiologia
Genes de Plantas
Ligações de Hidrogênio
Humanos
Concentração de Íons de Hidrogênio
Modelos Moleculares
Reação em Cadeia da Polimerase
dc.subject.decs.pt_BR.fl_str_mv Fabaceae/genética
Lectinas/genética
Plantas Medicinais
Sequência de Aminoácidos
Animais
Sequência de Bases
Sítios de Ligação
Clonagem Molecular
Primers do DNA/genética
Edema/etiologia
Genes de Plantas
Ligações de Hidrogênio
Humanos
Concentração de Íons de Hidrogênio
Modelos Moleculares
Reação em Cadeia da Polimerase
description Universidade Federal do Ceará. Departamento de Bioquimica e Biologia Molecular. Laboratdrio de Lectinas. Centro de Ciências. Fortaleza, CE, Brasil
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2014-03-26T14:05:44Z
dc.date.available.fl_str_mv 2014-03-26T14:05:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GRANGEIRO, T. B. et al. Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds. Eur.opean Journal of Biochemistry, v. 248, n. 1, p. 43-48, 1997.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7449
dc.identifier.issn.none.fl_str_mv 0014-2956
identifier_str_mv GRANGEIRO, T. B. et al. Molecular cloning and characterization of ConBr, the lectin of Canavalia brasiliensis seeds. Eur.opean Journal of Biochemistry, v. 248, n. 1, p. 43-48, 1997.
0014-2956
url https://www.arca.fiocruz.br/handle/icict/7449
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7449/1/Grangeiro%20TB%20MOlecular%20cloning....pdf
https://www.arca.fiocruz.br/bitstream/icict/7449/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7449/5/Grangeiro%20TB%20MOlecular%20cloning....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7449/4/Grangeiro%20TB%20MOlecular%20cloning....pdf.jpg
bitstream.checksum.fl_str_mv 38c947d41b2ca3da027419d0ca6b500c
7d48279ffeed55da8dfe2f8e81f3b81f
537c6758b7133a7489ef38786832fa51
cf760a710ade33d7567befb3b8ada128
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324830388355072

Registros relacionados