Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test

Detalhes bibliográficos
Autor(a) principal: Matos, Denise Cristina Souza
Data de Publicação: 2002
Outros Autores: Marcovistz, Rugimar, Cabello, Pedro Herman, Georgini, Ricardo Amaral, Sakauchi, Dirce, Silva, Luciana Leite da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44211
Resumo: Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.
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spelling Matos, Denise Cristina SouzaMarcovistz, RugimarCabello, Pedro HermanGeorgini, Ricardo AmaralSakauchi, DirceSilva, Luciana Leite da2020-10-26T19:08:55Z2020-10-26T19:08:55Z2002MATOS, Denise Cristina Souza et al. Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 909-913, Sept. 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4421110.1590/S0074-027620020006000301678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Vacina contra difteria-tétanoVacina contra difteria-tétano-coquelucheTeste de inibição de ligação de toxinasTétanoControle de qualidadeSoroneutralizaçãoDiphtheria-tetanus vaccineDiphtheria-tetanus-pertussis vaccineToxin binding inhibition testTetanusQuality controlSeroneutralizationImmunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanuspertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44211/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPedroHernan_Cabello_etal_IOC_2002.pdfPedroHernan_Cabello_etal_IOC_2002.pdfapplication/pdf39138https://www.arca.fiocruz.br/bitstream/icict/44211/2/PedroHernan_Cabello_etal_IOC_2002.pdf49a0008549d440cf275620c972d06c05MD52TEXTPedroHernan_Cabello_etal_IOC_2002.pdf.txtPedroHernan_Cabello_etal_IOC_2002.pdf.txtExtracted texttext/plain23554https://www.arca.fiocruz.br/bitstream/icict/44211/3/PedroHernan_Cabello_etal_IOC_2002.pdf.txt908dbb1bd21d7a599844ce9960a682eeMD53icict/442112020-10-27 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dc.title.pt_BR.fl_str_mv Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
title Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
spellingShingle Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
Matos, Denise Cristina Souza
Vacina contra difteria-tétano
Vacina contra difteria-tétano-coqueluche
Teste de inibição de ligação de toxinas
Tétano
Controle de qualidade
Soroneutralização
Diphtheria-tetanus vaccine
Diphtheria-tetanus-pertussis vaccine
Toxin binding inhibition test
Tetanus
Quality control
Seroneutralization
title_short Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
title_full Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
title_fullStr Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
title_full_unstemmed Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
title_sort Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test
author Matos, Denise Cristina Souza
author_facet Matos, Denise Cristina Souza
Marcovistz, Rugimar
Cabello, Pedro Herman
Georgini, Ricardo Amaral
Sakauchi, Dirce
Silva, Luciana Leite da
author_role author
author2 Marcovistz, Rugimar
Cabello, Pedro Herman
Georgini, Ricardo Amaral
Sakauchi, Dirce
Silva, Luciana Leite da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Matos, Denise Cristina Souza
Marcovistz, Rugimar
Cabello, Pedro Herman
Georgini, Ricardo Amaral
Sakauchi, Dirce
Silva, Luciana Leite da
dc.subject.other.pt_BR.fl_str_mv Vacina contra difteria-tétano
Vacina contra difteria-tétano-coqueluche
Teste de inibição de ligação de toxinas
Tétano
Controle de qualidade
Soroneutralização
topic Vacina contra difteria-tétano
Vacina contra difteria-tétano-coqueluche
Teste de inibição de ligação de toxinas
Tétano
Controle de qualidade
Soroneutralização
Diphtheria-tetanus vaccine
Diphtheria-tetanus-pertussis vaccine
Toxin binding inhibition test
Tetanus
Quality control
Seroneutralization
dc.subject.en.pt_BR.fl_str_mv Diphtheria-tetanus vaccine
Diphtheria-tetanus-pertussis vaccine
Toxin binding inhibition test
Tetanus
Quality control
Seroneutralization
description Instituto Butantan. Serviço de Controle de Qualidade. São Paulo, SP, Brasil.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-10-26T19:08:55Z
dc.date.available.fl_str_mv 2020-10-26T19:08:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MATOS, Denise Cristina Souza et al. Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 909-913, Sept. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44211
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000600030
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MATOS, Denise Cristina Souza et al. Immunogenicity Test of Tetanus Component in Adsorbed Vaccines by Toxin Binding Inhibition Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 909-913, Sept. 2002.
0074-0276
10.1590/S0074-02762002000600030
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44211
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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