Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test

Detalhes bibliográficos
Autor(a) principal: Matos,Denise Cristina Souza
Data de Publicação: 2002
Outros Autores: Marcovistz,Rugimar, Cabello,Pedro Hernan, Georgini,Ricardo Amaral, Sakauchi,Dirce, Silva,Luciana Leite da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600030
Resumo: Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.
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spelling Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition testdiphtheria-tetanus vaccinediphtheria-tetanus-pertussis vaccinetoxin binding inhibition testtetanusquality controlseroneutralizationSamples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.Instituto Oswaldo Cruz, Ministério da Saúde2002-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600030Memórias do Instituto Oswaldo Cruz v.97 n.6 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000600030info:eu-repo/semantics/openAccessMatos,Denise Cristina SouzaMarcovistz,RugimarCabello,Pedro HernanGeorgini,Ricardo AmaralSakauchi,DirceSilva,Luciana Leite daeng2020-04-25T17:48:51Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:22.994Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
title Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
spellingShingle Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
Matos,Denise Cristina Souza
diphtheria-tetanus vaccine
diphtheria-tetanus-pertussis vaccine
toxin binding inhibition test
tetanus
quality control
seroneutralization
title_short Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
title_full Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
title_fullStr Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
title_full_unstemmed Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
title_sort Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test
author Matos,Denise Cristina Souza
author_facet Matos,Denise Cristina Souza
Marcovistz,Rugimar
Cabello,Pedro Hernan
Georgini,Ricardo Amaral
Sakauchi,Dirce
Silva,Luciana Leite da
author_role author
author2 Marcovistz,Rugimar
Cabello,Pedro Hernan
Georgini,Ricardo Amaral
Sakauchi,Dirce
Silva,Luciana Leite da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Matos,Denise Cristina Souza
Marcovistz,Rugimar
Cabello,Pedro Hernan
Georgini,Ricardo Amaral
Sakauchi,Dirce
Silva,Luciana Leite da
dc.subject.por.fl_str_mv diphtheria-tetanus vaccine
diphtheria-tetanus-pertussis vaccine
toxin binding inhibition test
tetanus
quality control
seroneutralization
topic diphtheria-tetanus vaccine
diphtheria-tetanus-pertussis vaccine
toxin binding inhibition test
tetanus
quality control
seroneutralization
dc.description.none.fl_txt_mv Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.
description Samples from 20 lots of diphtheria-tetanus (adult use dT) vaccine and from 20 lots of diphtheria-tetanus-pertussis (DTP) vaccine were used to standardize and validate the in vitro toxin binding inhibition (ToBI) test for the immunogenicity test of the tetanus component. The levels of tetanus antitoxin obtained by ToBI test were compared to those obtained using the toxin neutralization (TN) test in mice routinely employed to perform the quality control of the tetanus component in adsorbed vaccines. The results ranged from 1.8 to 3.5 IU/ml for dT and 2 to 4 IU/ml for DTP by ToBI test and 1.4 to 3 IU/ml for dT and 1.8 to 3.5 IU/ml for DTP by TN in mice. These results were significantly correlated. From this study, it is concluded that the ToBI test is an alternative to the in vivo neutralization procedure in the immunogenicity test of the tetanus component in adsorbed vaccines. A substantial refinement and a reduction in use of animals can be achieved.
publishDate 2002
dc.date.none.fl_str_mv 2002-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600030
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600030
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000600030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.6 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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