Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Alves, Lysangela Ronalte
Data de Publicação: 2015
Outros Autores: Antes, Camila Olieira, Goldenberg, Samuel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15501
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Alves, Lysangela RonalteAntes, Camila OlieiraGoldenberg, Samuel2016-08-29T17:13:21Z2016-08-29T17:13:21Z2015ALVES, Lysangela Ronalte; OLIVEIRA, Camila; GOLDENBERG, Samuel. Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi. BMC Microbiology, v.15, n.104, p. 1-9, 2015.1471-2180https://www.arca.fiocruz.br/handle/icict/15501porEukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Background Regulation of gene expression in trypanosomatids is mainly posttranscriptional. Tight regulation of mRNA stability and access to polysomes allows Trypanosoma cruzi to adapt to different environmental conditions during its life cycle. Posttranscriptional regulation requires association between mRNAs and specific proteins to form mRNP complexes. Proteins that lack a canonical RNA-binding domain, such as eukaryotic elongation factor-1α (EF-1α), may also associate with mRNPs. EF-1α is conserved in many organisms, and it plays roles in many cellular processes other than translation, including RNA transport, the cell cycle, and apoptosis. Results In a previous study, EF-1α was found associated with mRNP-forming mRNAs in polysome-free fractions both in epimastigotes growing under normal conditions and in nutritionally stressed parasites. This finding suggested the possibility that EF-1α has a non-canonical function. Thus, we investigated the dynamics of EF-1α in association with T. cruzi epimastigote mRNAs under normal and stressed nutritional conditions. EF-1α is expressed throughout the parasite life cycle, but it shows a slight decrease in protein levels in the metacyclic trypomastigote form. The protein is cytoplasmically localized with a granular pattern in all forms analyzed. Following puromycin treatment, EF-1α migrated with the heaviest gradient fractions in a sucrose polysome profile, indicating that its association with large protein complexes was independent of the translation machinery. We next characterized the EF-1α-associated mRNAs in unstressed and stressed epimastigotes. We observed that specific subsets of mRNAs were associated with EF-1α-mRNPs in unstressed or stressed epimastigotes. Some mRNAs were identified in both physiological conditions, whereas others were condition-specific. Gene ontology analysis identified enrichment of gene sets involved in single-organism metabolic processes, amino acid metabolic processes, ATP and metal ion binding, glycolysis, glutamine metabolic processes, and cobalt and iron ion binding. Conclusion These results indicate that in T. cruzi, as in other eukaryotes, EF-1α may play a non-canonical cellular role. We observed the enrichment of functionally related transcripts bound to EF-1α in normal growth conditions as well as in nutritionally stressed cell indicating a potential role of EF-1α mRNP in stress response.Trypanosoma cruziGene expressionmRNPsEF-1 alphaNon-canonical functioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15501/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALEukaryotic translation elongation factor-1 alpha.pdfEukaryotic translation elongation factor-1 alpha.pdfapplication/pdf1374184https://www.arca.fiocruz.br/bitstream/icict/15501/2/Eukaryotic%20translation%20elongation%20factor-1%20alpha.pdf505eafd36a7b7004b1d31840ebe8b37eMD52TEXTEukaryotic translation elongation factor-1 alpha.pdf.txtEukaryotic translation elongation factor-1 alpha.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
title Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
spellingShingle Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
Alves, Lysangela Ronalte
Trypanosoma cruzi
Gene expression
mRNPs
EF-1 alpha
Non-canonical function
title_short Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
title_full Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
title_fullStr Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
title_full_unstemmed Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
title_sort Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
author Alves, Lysangela Ronalte
author_facet Alves, Lysangela Ronalte
Antes, Camila Olieira
Goldenberg, Samuel
author_role author
author2 Antes, Camila Olieira
Goldenberg, Samuel
author2_role author
author
dc.contributor.author.fl_str_mv Alves, Lysangela Ronalte
Antes, Camila Olieira
Goldenberg, Samuel
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Gene expression
mRNPs
EF-1 alpha
Non-canonical function
topic Trypanosoma cruzi
Gene expression
mRNPs
EF-1 alpha
Non-canonical function
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-08-29T17:13:21Z
dc.date.available.fl_str_mv 2016-08-29T17:13:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Lysangela Ronalte; OLIVEIRA, Camila; GOLDENBERG, Samuel. Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi. BMC Microbiology, v.15, n.104, p. 1-9, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15501
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
identifier_str_mv ALVES, Lysangela Ronalte; OLIVEIRA, Camila; GOLDENBERG, Samuel. Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi. BMC Microbiology, v.15, n.104, p. 1-9, 2015.
1471-2180
url https://www.arca.fiocruz.br/handle/icict/15501
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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