Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides

Detalhes bibliográficos
Autor(a) principal: Bahia, D.
Data de Publicação: 1999
Outros Autores: Font, J., Khaouja, A., Carreras, N., Espuny, R., Cicarelli, RMB, Ingelmo, M., Bach-Elias, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1046/j.1432-1327.1999.00287.x
http://hdl.handle.net/11449/7871
Resumo: Two regions common to all UsnRNP core polypeptides have been described: Sm motif 1 and Sm motif 2. Rabbits were immunized with a 22 amino-acid peptide containing one segment of Sm motif 1 (YRGTLVSTDNYFNLQL-NEAEEF, corresponding to residues 11-32) from yeast F protein. After immunization, the rabbit sera contained antibodies that not only reacted specifically with the peptide from yeast F protein but also cross-reacted with Sm polypeptides from mammals; that is, with purified human U1snRNPs. The results suggest that the peptide used and human Sm polypeptides contain a common feature recognized by the polyclonal antibodies. A large collection of human systemic lupus erythematosus sera was assayed using the yeast peptide as an antigen source. Seventy per cent of systemic lupus erythematosus sera contain an antibody specificity that cross-reacts with the yeast peptide.
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spelling Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptidesautoimmunityepitope mappingpeptide-ELISASm motif 1UsnRNPsTwo regions common to all UsnRNP core polypeptides have been described: Sm motif 1 and Sm motif 2. Rabbits were immunized with a 22 amino-acid peptide containing one segment of Sm motif 1 (YRGTLVSTDNYFNLQL-NEAEEF, corresponding to residues 11-32) from yeast F protein. After immunization, the rabbit sera contained antibodies that not only reacted specifically with the peptide from yeast F protein but also cross-reacted with Sm polypeptides from mammals; that is, with purified human U1snRNPs. The results suggest that the peptide used and human Sm polypeptides contain a common feature recognized by the polyclonal antibodies. A large collection of human systemic lupus erythematosus sera was assayed using the yeast peptide as an antigen source. Seventy per cent of systemic lupus erythematosus sera contain an antibody specificity that cross-reacts with the yeast peptide.CSIC, IIBB, ES-08034 Barcelona, SpainHosp Clin & Prov Barcelona, Syst Autoimmune Dis Unit, Barcelona, SpainUNESP, Fac Ciências Farmaceut, Araraquara, SP, BrazilUNESP, Fac Ciências Farmaceut, Araraquara, SP, BrazilBlackwell ScienceCSICHosp Clin & Prov BarcelonaUniversidade Estadual Paulista (Unesp)Bahia, D.Font, J.Khaouja, A.Carreras, N.Espuny, R.Cicarelli, RMBIngelmo, M.Bach-Elias, M.2014-05-20T13:24:56Z2014-05-20T13:24:56Z1999-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article371-378application/pdfhttp://dx.doi.org/10.1046/j.1432-1327.1999.00287.xEuropean Journal of Biochemistry. Oxford: Blackwell Science Ltd, v. 261, n. 2, p. 371-378, 1999.0014-2956http://hdl.handle.net/11449/787110.1046/j.1432-1327.1999.00287.xWOS:000080471400004WOS000080471400004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Biochemistryinfo:eu-repo/semantics/openAccess2024-06-24T13:08:25Zoai:repositorio.unesp.br:11449/7871Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:08:56.878338Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
title Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
spellingShingle Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
Bahia, D.
autoimmunity
epitope mapping
peptide-ELISA
Sm motif 1
UsnRNPs
title_short Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
title_full Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
title_fullStr Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
title_full_unstemmed Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
title_sort Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides
author Bahia, D.
author_facet Bahia, D.
Font, J.
Khaouja, A.
Carreras, N.
Espuny, R.
Cicarelli, RMB
Ingelmo, M.
Bach-Elias, M.
author_role author
author2 Font, J.
Khaouja, A.
Carreras, N.
Espuny, R.
Cicarelli, RMB
Ingelmo, M.
Bach-Elias, M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv CSIC
Hosp Clin & Prov Barcelona
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Bahia, D.
Font, J.
Khaouja, A.
Carreras, N.
Espuny, R.
Cicarelli, RMB
Ingelmo, M.
Bach-Elias, M.
dc.subject.por.fl_str_mv autoimmunity
epitope mapping
peptide-ELISA
Sm motif 1
UsnRNPs
topic autoimmunity
epitope mapping
peptide-ELISA
Sm motif 1
UsnRNPs
description Two regions common to all UsnRNP core polypeptides have been described: Sm motif 1 and Sm motif 2. Rabbits were immunized with a 22 amino-acid peptide containing one segment of Sm motif 1 (YRGTLVSTDNYFNLQL-NEAEEF, corresponding to residues 11-32) from yeast F protein. After immunization, the rabbit sera contained antibodies that not only reacted specifically with the peptide from yeast F protein but also cross-reacted with Sm polypeptides from mammals; that is, with purified human U1snRNPs. The results suggest that the peptide used and human Sm polypeptides contain a common feature recognized by the polyclonal antibodies. A large collection of human systemic lupus erythematosus sera was assayed using the yeast peptide as an antigen source. Seventy per cent of systemic lupus erythematosus sera contain an antibody specificity that cross-reacts with the yeast peptide.
publishDate 1999
dc.date.none.fl_str_mv 1999-04-01
2014-05-20T13:24:56Z
2014-05-20T13:24:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1046/j.1432-1327.1999.00287.x
European Journal of Biochemistry. Oxford: Blackwell Science Ltd, v. 261, n. 2, p. 371-378, 1999.
0014-2956
http://hdl.handle.net/11449/7871
10.1046/j.1432-1327.1999.00287.x
WOS:000080471400004
WOS000080471400004.pdf
url http://dx.doi.org/10.1046/j.1432-1327.1999.00287.x
http://hdl.handle.net/11449/7871
identifier_str_mv European Journal of Biochemistry. Oxford: Blackwell Science Ltd, v. 261, n. 2, p. 371-378, 1999.
0014-2956
10.1046/j.1432-1327.1999.00287.x
WOS:000080471400004
WOS000080471400004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 371-378
application/pdf
dc.publisher.none.fl_str_mv Blackwell Science
publisher.none.fl_str_mv Blackwell Science
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129494831071232

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