The Brazilian network for HIV-1 genotyping external quality control assurance programme

Detalhes bibliográficos
Autor(a) principal: Souza, Denise C. F.
Data de Publicação: 2011
Outros Autores: Sucupira, Maria Cecilia A., Brindeiro, Rodrigo M., Fernandez, José Carlos C., Sabino, Ester C., Inocencio, Lilian A., Diaz, Ricardo S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15871
Resumo: Ministério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil.
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spelling Souza, Denise C. F.Sucupira, Maria Cecilia A.Brindeiro, Rodrigo M.Fernandez, José Carlos C.Sabino, Ester C.Inocencio, Lilian A.Diaz, Ricardo S.2016-09-20T16:33:53Z2016-09-20T16:33:53Z2011SOUZA, Denise C. F. et al. The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme. Journal of the International AIDS Society, v.14, p. 1-6, 2011.1758-2652https://www.arca.fiocruz.br/handle/icict/1587110.1186/1758-2652-14-45engBioMed CentralHIV-1Coinfecção por HIVRede BrasileiraGenotipagem HIV-1Brazilian NetworkHIV-1 GenotypingHIV-infected patientsThe Brazilian network for HIV-1 genotyping external quality control assurance programmeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMinistério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Pro-Sangue. Hemocentro de São Paulo. São Paulo, SP, Brasil.Ministério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil .Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil.The Brazilian network for genotyping is composed of 21 laboratories that perform and analyze genotyping tests for all HIV-infected patients within the public system, performing approximately 25,000 tests per year. We assessed the interlaboratory and intralaboratory reproducibility of genotyping systems by creating and implementing a local external quality control evaluation. Plasma samples from HIV-1-infected individuals (with low and intermediate viral loads) or RNA viral constructs with specific mutations were used. This evaluation included analyses of sensitivity and specificity of the tests based on qualitative and quantitative criteria, which scored laboratory performance on a 100-point system. Five evaluations were performed from 2003 to 2008, with 64% of laboratories scoring over 80 points in 2003, 81% doing so in 2005, 56% in 2006, 91% in 2007, and 90% in 2008 (Kruskal-Wallis, p = 0.003). Increased performance was aided by retraining laboratories that had specific deficiencies. The results emphasize the importance of investing in laboratory training and interpretation of DNA sequencing results, especially in developing countries where public (or scarce) resources are used to manage the AIDS epidemic.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15871/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjose_fernandez_etal_IOC_2011.pdfjose_fernandez_etal_IOC_2011.pdfapplication/pdf242206https://www.arca.fiocruz.br/bitstream/icict/15871/2/jose_fernandez_etal_IOC_2011.pdf0af37c679a16ccc1046c495e04bd006fMD52TEXTjose_fernandez_etal_IOC_2011.pdf.txtjose_fernandez_etal_IOC_2011.pdf.txtExtracted texttext/plain26510https://www.arca.fiocruz.br/bitstream/icict/15871/3/jose_fernandez_etal_IOC_2011.pdf.txt4a864ae9521c1fe84d17da03cf1f8f44MD53icict/158712020-02-05 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dc.title.pt_BR.fl_str_mv The Brazilian network for HIV-1 genotyping external quality control assurance programme
title The Brazilian network for HIV-1 genotyping external quality control assurance programme
spellingShingle The Brazilian network for HIV-1 genotyping external quality control assurance programme
Souza, Denise C. F.
HIV-1
Coinfecção por HIV
Rede Brasileira
Genotipagem HIV-1
Brazilian Network
HIV-1 Genotyping
HIV-infected patients
title_short The Brazilian network for HIV-1 genotyping external quality control assurance programme
title_full The Brazilian network for HIV-1 genotyping external quality control assurance programme
title_fullStr The Brazilian network for HIV-1 genotyping external quality control assurance programme
title_full_unstemmed The Brazilian network for HIV-1 genotyping external quality control assurance programme
title_sort The Brazilian network for HIV-1 genotyping external quality control assurance programme
author Souza, Denise C. F.
author_facet Souza, Denise C. F.
Sucupira, Maria Cecilia A.
Brindeiro, Rodrigo M.
Fernandez, José Carlos C.
Sabino, Ester C.
Inocencio, Lilian A.
Diaz, Ricardo S.
author_role author
author2 Sucupira, Maria Cecilia A.
Brindeiro, Rodrigo M.
Fernandez, José Carlos C.
Sabino, Ester C.
Inocencio, Lilian A.
Diaz, Ricardo S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Denise C. F.
Sucupira, Maria Cecilia A.
Brindeiro, Rodrigo M.
Fernandez, José Carlos C.
Sabino, Ester C.
Inocencio, Lilian A.
Diaz, Ricardo S.
dc.subject.other.pt_BR.fl_str_mv HIV-1
Coinfecção por HIV
Rede Brasileira
Genotipagem HIV-1
topic HIV-1
Coinfecção por HIV
Rede Brasileira
Genotipagem HIV-1
Brazilian Network
HIV-1 Genotyping
HIV-infected patients
dc.subject.en.pt_BR.fl_str_mv Brazilian Network
HIV-1 Genotyping
HIV-infected patients
description Ministério da Saúde. Programa Brasileiro de DST / AIDS e Hepatites vírus. Brasília, DF, Brasil / Universidade Federal de São Paulo. Laboratório de Retrovirologia. São Paulo, SP, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-09-20T16:33:53Z
dc.date.available.fl_str_mv 2016-09-20T16:33:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Denise C. F. et al. The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme. Journal of the International AIDS Society, v.14, p. 1-6, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15871
dc.identifier.issn.pt_BR.fl_str_mv 1758-2652
dc.identifier.doi.none.fl_str_mv 10.1186/1758-2652-14-45
identifier_str_mv SOUZA, Denise C. F. et al. The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme. Journal of the International AIDS Society, v.14, p. 1-6, 2011.
1758-2652
10.1186/1758-2652-14-45
url https://www.arca.fiocruz.br/handle/icict/15871
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15871/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15871/2/jose_fernandez_etal_IOC_2011.pdf
https://www.arca.fiocruz.br/bitstream/icict/15871/3/jose_fernandez_etal_IOC_2011.pdf.txt
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