Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene

Detalhes bibliográficos
Autor(a) principal: Nerys Junior, Arildo
Data de Publicação: 2018
Outros Autores: Dias, Luciene P. Braga, Pezzuto, Paula, Almeida, Vinícius Cotta de, Tanuri, Amilcar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28639
Resumo: Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Nerys Junior, ArildoDias, Luciene P. BragaPezzuto, PaulaAlmeida, Vinícius Cotta deTanuri, Amilcar2018-09-11T13:21:19Z2018-09-11T13:21:19Z2018NERYS JUNIOR, Arildo; et al. Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene. Genetics and Molecular Biology, v.41, n.1, p.167-179, 2018.1415-4757https://www.arca.fiocruz.br/handle/icict/2863910.1590/1678-4685-GMB-2017-00651678-4685engSociedade Brasileira de GenéticaComparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Ciência e Tecnologia em Biomodelos. Laboratório do Serviço de Biotecnologia e Desenvolvimento Animal. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.The human C-C chemokine receptor type-5 (CCR5) is the major transmembrane co-receptor that mediates HIV-1 entry into target CD4+ cells. Gene therapy to knock-out the CCR5 gene has shown encouraging results in providing a functional cure for HIV-1 infection. In gene therapy strategies, the initial region of the CCR5 gene is a hotspot for producing functional gene knock-out. Such target gene editing can be done using programmable endonucleases such as transcription activator-like effector nucleases (TALEN) or clustered regularly interspaced short palindromic repeats (CRISPR-Cas9). These two gene editing approaches are the most modern and effective tools for precise gene modification. However, little is known of potential differences in the efficiencies of TALEN and CRISPR-Cas9 for editing the beginning of the CCR5 gene. To examine which of these two methods is best for gene therapy, we compared the patterns and amount of editing at the beginning of the CCR5 gene using TALEN and CRISPR-Cas9 followed by DNA sequencing. This comparison revealed that CRISPR-Cas9 mediated the sorting of cells that contained 4.8 times more gene editing than TALEN+ transfected cells.CCR5CRISPR-Cas9efficiencygene editingTALENNucleases dos Efetores Semelhantes a Ativadores de TranscriçãoSistemas CRISPR-CasReceptores CCR5Eficiênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28639/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALviniciusc_almeida_etal_IOC_2018.pdfviniciusc_almeida_etal_IOC_2018.pdfapplication/pdf2556093https://www.arca.fiocruz.br/bitstream/icict/28639/2/viniciusc_almeida_etal_IOC_2018.pdfe2dcab94a74ba8ffdc1c45b3170a06b2MD52TEXTviniciusc_almeida_etal_IOC_2018.pdf.txtviniciusc_almeida_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
title Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
spellingShingle Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
Nerys Junior, Arildo
CCR5
CRISPR-Cas9
efficiency
gene editing
TALEN
Nucleases dos Efetores Semelhantes a Ativadores de Transcrição
Sistemas CRISPR-Cas
Receptores CCR5
Eficiência
title_short Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
title_full Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
title_fullStr Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
title_full_unstemmed Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
title_sort Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene
author Nerys Junior, Arildo
author_facet Nerys Junior, Arildo
Dias, Luciene P. Braga
Pezzuto, Paula
Almeida, Vinícius Cotta de
Tanuri, Amilcar
author_role author
author2 Dias, Luciene P. Braga
Pezzuto, Paula
Almeida, Vinícius Cotta de
Tanuri, Amilcar
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nerys Junior, Arildo
Dias, Luciene P. Braga
Pezzuto, Paula
Almeida, Vinícius Cotta de
Tanuri, Amilcar
dc.subject.en.pt_BR.fl_str_mv CCR5
CRISPR-Cas9
efficiency
gene editing
TALEN
topic CCR5
CRISPR-Cas9
efficiency
gene editing
TALEN
Nucleases dos Efetores Semelhantes a Ativadores de Transcrição
Sistemas CRISPR-Cas
Receptores CCR5
Eficiência
dc.subject.decs.pt_BR.fl_str_mv Nucleases dos Efetores Semelhantes a Ativadores de Transcrição
Sistemas CRISPR-Cas
Receptores CCR5
Eficiência
description Universidade Federal do Rio de Janeiro. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-11T13:21:19Z
dc.date.available.fl_str_mv 2018-09-11T13:21:19Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NERYS JUNIOR, Arildo; et al. Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene. Genetics and Molecular Biology, v.41, n.1, p.167-179, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28639
dc.identifier.issn.pt_BR.fl_str_mv 1415-4757
dc.identifier.doi.none.fl_str_mv 10.1590/1678-4685-GMB-2017-0065
dc.identifier.eissn.none.fl_str_mv 1678-4685
identifier_str_mv NERYS JUNIOR, Arildo; et al. Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene. Genetics and Molecular Biology, v.41, n.1, p.167-179, 2018.
1415-4757
10.1590/1678-4685-GMB-2017-0065
1678-4685
url https://www.arca.fiocruz.br/handle/icict/28639
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28639/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28639/2/viniciusc_almeida_etal_IOC_2018.pdf
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