Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia

Detalhes bibliográficos
Autor(a) principal: Moreira, Luciano Andrade
Data de Publicação: 2009
Outros Autores: Saig, Emad, Turley, Andrew P., Ribeiro, Jose M. C., O’Neill, Scott L., McGraw, Elizabeth A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58801
Resumo: School of Biological Sciences. The University of Queensland. St. Lucia, Queensland, Australia/Laboratory of Malaria. Rene Rachou Research Center.Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil
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spelling Moreira, Luciano AndradeSaig, EmadTurley, Andrew P.Ribeiro, Jose M. C.O’Neill, Scott L.McGraw, Elizabeth A.2023-05-29T17:10:36Z2023-05-29T17:10:36Z2009MOREIRA, Luciano Andrade et al. Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia. PLoS Negl Trop Dis., v. 3, n. 12, e568, 2009. doi: 10.1371/journal.pntd.0000568.1935-2735https://www.arca.fiocruz.br/handle/icict/5880110.1371/journal.pntd.0000568engPublic Library of ScienceHuman Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSchool of Biological Sciences. The University of Queensland. St. Lucia, Queensland, Australia/Laboratory of Malaria. Rene Rachou Research Center.Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilSchool of Biological Sciences. The University of Queensland. St. Lucia, Queensland, AustraliaSchool of Biological Sciences. The University of Queensland. St. Lucia, Queensland, AustraliaLaboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Rockville, Maryland, United States of AmericaSchool of Biological Sciences. The University of Queensland. St. Lucia, Queensland, AustraliaSchool of Biological Sciences. The University of Queensland. St. Lucia, Queensland, AustraliaBackground: Mosquitoes are vectors of many serious pathogens in tropical and sub-tropical countries. Current control strategies almost entirely rely upon insecticides, which increasingly face the problems of high cost, increasing mosquito resistance and negative effects on non-target organisms. Alternative strategies include the proposed use of inherited life-shortening agents, such as the Wolbachia bacterium. By shortening mosquito vector lifespan, Wolbachia could potentially reduce the vectorial capacity of mosquito populations. We have recently been able to stably transinfect Aedes aegypti mosquitoes with the life-shortening Wolbachia strain wMelPop, and are assessing various aspects of its interaction with the mosquito host to determine its likely impact on pathogen transmission as well as its potential ability to invade A. aegypti populations. Methodology/Principal Findings: Here we have examined the probing behavior of Wolbachia-infected mosquitoes in an attempt to understand both the broader impact of Wolbachia infection on mosquito biology and, in particular, vectorial capacity. The probing behavior of wMelPop-infected mosquitoes at four adult ages was examined and compared to uninfected controls during video-recorded feeding trials on a human hand. Wolbachia-positive insects, from 15 days of age, showed a drastic increase in the time spent pre-probing and probing relative to uninfected controls. Two other important features for blood feeding, saliva volume and apyrase content of saliva, were also studied. Conclusions/Significance: As A. aegypti infected with wMelPop age, they show increasing difficulty in completing the process of blood feeding effectively and efficiently. Wolbachia-infected mosquitoes on average produced smaller volumes of saliva that still contained the same amount of apyrase activity as uninfected mosquitoes. These effects on blood feeding behavior may reduce vectorial capacity and point to underlying physiological changes in Wolbachia-infected mosquitoesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpntd.0000568 1..6.pdfpntd.0000568 1..6.pdfapplication/pdf210022https://www.arca.fiocruz.br/bitstream/icict/58801/2/pntd.0000568%201..6.pdf57c3b100770b81f3bb4b36717fe34c74MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58801/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/588012023-05-29 14:10:37.657oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-29T17:10:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
title Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
spellingShingle Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
Moreira, Luciano Andrade
title_short Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
title_full Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
title_fullStr Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
title_full_unstemmed Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
title_sort Human Probing Behavior of Aedes aegypti when Infected with a Life-Shortening Strain of Wolbachia
author Moreira, Luciano Andrade
author_facet Moreira, Luciano Andrade
Saig, Emad
Turley, Andrew P.
Ribeiro, Jose M. C.
O’Neill, Scott L.
McGraw, Elizabeth A.
author_role author
author2 Saig, Emad
Turley, Andrew P.
Ribeiro, Jose M. C.
O’Neill, Scott L.
McGraw, Elizabeth A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira, Luciano Andrade
Saig, Emad
Turley, Andrew P.
Ribeiro, Jose M. C.
O’Neill, Scott L.
McGraw, Elizabeth A.
description School of Biological Sciences. The University of Queensland. St. Lucia, Queensland, Australia/Laboratory of Malaria. Rene Rachou Research Center.Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2023-05-29T17:10:36Z
dc.date.available.fl_str_mv 2023-05-29T17:10:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Luciano Andrade et al. Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia. PLoS Negl Trop Dis., v. 3, n. 12, e568, 2009. doi: 10.1371/journal.pntd.0000568.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58801
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0000568
identifier_str_mv MOREIRA, Luciano Andrade et al. Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia. PLoS Negl Trop Dis., v. 3, n. 12, e568, 2009. doi: 10.1371/journal.pntd.0000568.
1935-2735
10.1371/journal.pntd.0000568
url https://www.arca.fiocruz.br/handle/icict/58801
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58801/2/pntd.0000568%201..6.pdf
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