Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study

Detalhes bibliográficos
Autor(a) principal: Leon, Luciane Almeida Amado
Data de Publicação: 2015
Outros Autores: Almeida, Adilson José de, Paula, Vanessa Salete de, Tourinho, Renata Santos, Villela, Daniel Antunes Maciel, Gaspar, Ana Maria Coimbra, Lewis-Ximenez, Lia Laura, Pinto, Marcelo Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13782
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Leon, Luciane Almeida AmadoAlmeida, Adilson José dePaula, Vanessa Salete deTourinho, Renata SantosVillela, Daniel Antunes MacielGaspar, Ana Maria CoimbraLewis-Ximenez, Lia LauraPinto, Marcelo Alves2016-04-14T17:41:15Z2016-04-14T17:41:15Z2015LEON, Luciane Almeida Amado; et al. Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study. Plos One, v.10, n.12, e0145454, Dec. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1378210.1371/journal.pone.0145454engPublic Library of ScienceLongitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica (PROCC). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Despite the increasing numbers of studies investigating hepatitis A diagnostic through saliva, the frequency and the pattern of hepatitis A virus (HAV) markers in this fluid still remains unknown. To address this issue, we carried on a longitudinal study to examine the kinetics of HAV markers in saliva, in comparison with serum samples. The present study followed-up ten patients with acute hepatitis A infection during 180 days post diagnosis (dpd). Total anti-HAV was detected in paired serum and saliva samples until the end of the follow-up, showing a peak titer at 90th. However, total anti-HAV level was higher in serum than in saliva samples. This HAV marker showed a probability of 100% to be detected in both serum and saliva during 180 dpd. The IgM anti-HAV could be detected in saliva up to 150 dpd, showing the highest frequency at 30th, when it was detected in all individuals. During the first month of HAV infection, this acute HAV marker showed a detection probability of 100% in paired samples. The detection of IgM anti-HAV in saliva was not dependent on its level in serum, HAV-RNA detection and/or viral load, since no association was found between IgM anti-HAV positivity in saliva and any of these parameter (p>0.05). Most of the patients (80%) were found to contain HAV-RNA in saliva, mainly at early acute phase (30th day). However, it was possible to demonstrate the HAV RNA presence in paired samples for more than 90 days, even after seroconversion. No significant relationship was observed between salivary HAV-RNA positivity and serum viral load, demonstrating that serum viral load is not predictive of HAV-RNA detection in saliva. Similar viral load was seen in paired samples (on average 104 copies/mL). These data demonstrate that the best diagnostic coverage can be achieved by salivary anti-HAV antibodies and HAV-RNA tests during 30–90 dpd. The long detection and high probability of specific-HAV antibodies positivity in saliva samples make the assessment of salivary antibodies a useful tool for diagnosis and epidemiological studies. The high frequency of HAV-RNA in saliva and the probability of detection of about 50%, during the first 30 dpd, demonstrate that saliva is also useful for molecular investigation of hepatitis A cases, mainly during the early course of infection. Therefore, the collection of saliva may provide a simple, cheap and non-invasive means of diagnosis, epidemiological surveys and monitoring of hepatitis A infection purposes.hepatitis A virus (HAV)Saliva SamplingHepatitis A InfectionVírus da Hepatite ASalivaAmostragemInfecçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13782/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALluciane_leon_etal_IOC_2015.pdfluciane_leon_etal_IOC_2015.pdfapplication/pdf796402https://www.arca.fiocruz.br/bitstream/icict/13782/2/luciane_leon_etal_IOC_2015.pdf3439650097292e43eb86412ce15b4a78MD52TEXTluciane_leon_etal_IOC_2015.pdf.txtluciane_leon_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
title Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
spellingShingle Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
Leon, Luciane Almeida Amado
hepatitis A virus (HAV)
Saliva Sampling
Hepatitis A Infection
Vírus da Hepatite A
Saliva
Amostragem
Infecção
title_short Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
title_full Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
title_fullStr Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
title_full_unstemmed Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
title_sort Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study
author Leon, Luciane Almeida Amado
author_facet Leon, Luciane Almeida Amado
Almeida, Adilson José de
Paula, Vanessa Salete de
Tourinho, Renata Santos
Villela, Daniel Antunes Maciel
Gaspar, Ana Maria Coimbra
Lewis-Ximenez, Lia Laura
Pinto, Marcelo Alves
author_role author
author2 Almeida, Adilson José de
Paula, Vanessa Salete de
Tourinho, Renata Santos
Villela, Daniel Antunes Maciel
Gaspar, Ana Maria Coimbra
Lewis-Ximenez, Lia Laura
Pinto, Marcelo Alves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leon, Luciane Almeida Amado
Almeida, Adilson José de
Paula, Vanessa Salete de
Tourinho, Renata Santos
Villela, Daniel Antunes Maciel
Gaspar, Ana Maria Coimbra
Lewis-Ximenez, Lia Laura
Pinto, Marcelo Alves
dc.subject.en.pt_BR.fl_str_mv hepatitis A virus (HAV)
Saliva Sampling
Hepatitis A Infection
topic hepatitis A virus (HAV)
Saliva Sampling
Hepatitis A Infection
Vírus da Hepatite A
Saliva
Amostragem
Infecção
dc.subject.decs.pt_BR.fl_str_mv Vírus da Hepatite A
Saliva
Amostragem
Infecção
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-14T17:41:15Z
dc.date.available.fl_str_mv 2016-04-14T17:41:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LEON, Luciane Almeida Amado; et al. Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study. Plos One, v.10, n.12, e0145454, Dec. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13782
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0145454
identifier_str_mv LEON, Luciane Almeida Amado; et al. Longitudinal Study of Hepatitis A Infection by Saliva Sampling: The Kinetics of HAV Markers in Saliva Revealed the Application of Saliva Tests for Hepatitis A Study. Plos One, v.10, n.12, e0145454, Dec. 2015.
1932-6203
10.1371/journal.pone.0145454
url https://www.arca.fiocruz.br/handle/icict/13782
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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